Fast and Green - CO2 Based Extraction, Isolation, and Quantification of Phenolic Styrax Constituents.

Abstract:

:In this study the first supercritical fluid based protocol for the extraction, analysis, and isolation of six polar compounds, i.e., o-vanillin (1), styracin (2), vanillin (3), trans-cinnamic acid (4), vanillic acid (5), and shikimic acid (6), was developed. First, eight styrax resin products (R1-R8) obtained from various Liquidambar tree species, which are known to contain compounds 2-6, were extracted with a 1 : 1 mixture of supercritical CO2 and EtOH. Within 4 minutes, the compounds were successfully baseline separated on an Acquity UPC2 BEH 2-EP (3.0 × 100 mm, 1.7 µm) column using a mobile phase of supercritical CO2 and MeOH with 0.1 % phosphoric acid. The compounds were quantified and the method was validated according to current ICH guidelines. Scaling up to preparative supercritical fluid chromatography using a Viridis BEH 2-EP (10 × 250 mm, 5 µm) column allowed for a fast separation and isolation of the selected constituents 2 and 4 from R6 within 7 minutes. This supercritical fluid protocol is easily adaptable to compounds of similar polarity. The increase in speed and its environmental friendliness underline its superiority over conventional set-ups.

journal_name

Planta Med

journal_title

Planta medica

authors

Scheuba J,Wronski VK,Rollinger JM,Grienke U

doi

10.1055/s-0043-105499

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

1068-1075

issue

12-13

eissn

0032-0943

issn

1439-0221

journal_volume

83

pub_type

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