Rapid qualitative and quantitative determination of seven valuable taxanes from various Taxus species by UFLC-ESI-MS and UFLC-DAD.

Abstract:

:The distribution and level of yew constituents vary with species and tissues. In this study, a rapid and valid method incorporating ultra-fast liquid chromatography (UFLC) with MS and UV detection was developed for simultaneous determination of paclitaxel and its six semisynthesis precursors in needles and hair roots from various Taxus species. All target analytes could be identified by comparing their retention times as well as UV and MS spectra with authentic standards, while seven valuable taxanes in botanical samples can be rapidly determined by UFLC-DAD with excellent sensitivity. Analysis of more than one hundred yew samples from nine species showed significant variations in distribution and content of seven evaluated taxanes. Thus, different developmental schemes should be used for better utilization of various yew resources.

journal_name

Planta Med

journal_title

Planta medica

authors

Ge GB,Liang SC,Hu Y,Liu XB,Mao YX,Zhang YY,Luan HW,Qiu MH,Yang L

doi

10.1055/s-0030-1249959

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1773-7

issue

15

eissn

0032-0943

issn

1439-0221

journal_volume

76

pub_type

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