An anti-inflammatory ent-kaurane from the stems of Annona squamosa that inhibits various human neutrophil functions.


:We have previously shown that 11 ent-kauranes isolated from the stems of Annona squamosa exhibited immunomodulating effects in leukocytes. In this study, a cellular model using isolated human neutrophils, which are important in the pathogenesis of rheumatoid arthritis, ischemia-reperfusion injury, chronic obstructive pulmonary disease, asthma and other inflammatory diseases, was established in order to elucidate the anti-inflammatory functions of 16beta,17-dihydroxy-ent-kauran-19-oic acid (1). Reactive oxygen species (ROS) and granule proteases produced by neutrophils contribute to the pathogenesis of inflammatory diseases. Compound 1 inhibited the generation of superoxide anion, the formation of ROS, and the release of elastase in formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP)-activated human neutrophils in a concentration-dependent manner with IC (50) values of 3.95 +/- 0.68, 12.20 +/- 2.16, and 12.52 +/- 2.26 microM, respectively. The anti-inflammatory actions were not attributable to cytotoxicity because incubation of the neutrophils with 1 did not result in lactate dehydrogenase release. Compound 1 did not display antioxidant or superoxide anion-scavenging activity. Furthermore, neither subcellular NADPH oxidase activity nor cAMP-dependent pathways were altered by 1. Compound 1 significantly inhibited rapid calcium release from internal calcium stores induced by FMLP but not by thapsigargin. In summary, the presented results indicate that the inhibitory effects of 1 on respiratory burst and degranulation of human neutrophils are through the inhibition of cytosolic calcium mobilization, but not via the cAMP-dependent pathways.


Planta Med


Planta medica


Yeh SH,Chang FR,Wu YC,Yang YL,Zhuo SK,Hwang TL




Has Abstract


2005-10-01 00:00:00












  • Stimulatory effects of ingenols from Euphorbia kansui on the expression of macrophage Fc receptor.

    abstract::Immune complex binding to macrophages was enhanced by treatment with an E. kansui extract. Systematic fractionation of the extract led to the characterization of 3-O-(2'E,4'Z-decadienoyl)- and 3-O-(2,3-dimethylbutyryl)-13- O-n-dodecanoyl-13-hydroxyingenol as the active principles. Immune complex binding to macrophages...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Matsumoto T,Cyong JC,Yamada H

    更新日期:1992-06-01 00:00:00

  • Seasonal variation of phenolic constituents and medicinal activities of Northern Labrador tea, Rhododendron tomentosum ssp. subarcticum, an Inuit and cree First Nations traditional medicine.

    abstract::Northern Labrador tea, Rhododendron tomentosum ssp. subarcticum, is one of the most commonly used medicinal plants by Inuit and other First Nations peoples of Canada. The phenolic profile and seasonal variation of this commonly used medicinal plant remains largely unknown. To assess optimal harvesting time, R. tomento...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Black P,Saleem A,Dunford A,Guerrero-Analco J,Walshe-Roussel B,Haddad P,Cuerrier A,Arnason JT

    更新日期:2011-09-01 00:00:00

  • Quantitative determination of ophiopogonin d by liquid chromatography/electrospray ionization mass spectrometry and its pharmacokinetics in rat.

    abstract::A sensitive and rapid liquid chromatography-mass spectrometric method for the determination of ophiopogonin D in rat plasma was developed and validated. Chromatographic separation was performed on a C (18) column using a step gradient program with the mobile phase of 0.5 mmol/L ammonium chloride solution and acetonitr...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Xia CH,Sun JG,Hao HP,Wang GJ,Yan B,Gu SH,Zheng CN,Shang LL,Roberts MS

    更新日期:2008-12-01 00:00:00

  • Isolation and Characterization of the Major Anthraquinone Pigments from Rumex abysinica.

    abstract::Emodin, chrysophanol and physcion have been shown to be the major anthraquinone pigments present in the dry roots of RUMEX ABYSINICA. ...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Munavu RM,Mudamba LO,Ogur JA

    更新日期:1984-02-01 00:00:00

  • Effects of Phaeodactylum tricornutum and Dunaliella tertiolecta extracts on the central nervous system.

    abstract::We report the effects of aqueous extracts of the microalgae Dunaliella tertiolecta Butcher (Chlorophyceae) and Phaeodactylum tricornutum Bohlin (Bacillariophyceae) on oxotremorin-induced cholinergic symptoms, amphetamine-induced hypermotility, pentylenetetrazole-induced convulsions, and Rota-rod test performance. Both...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Laguna MR,Villar RM,Cadavid I,Calleja JM

    更新日期:1990-04-01 00:00:00

  • Antiherpetic activities of sulfated polysaccharides from green algae.

    abstract::In order to evaluate the potency of novel antiviral drugs, 11 natural sulfated polysaccharides (SPs) from 10 green algae ( Enteromorpha compressa, Monostroma nitidum, Caulerpa brachypus, C. okamurai, C. scapelliformis, Chaetomorpha crassa, C. spiralis, Codium adhaerens, C. fragille, and C. latum) and 4 synthetic sulfa...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Lee JB,Hayashi K,Maeda M,Hayashi T

    更新日期:2004-09-01 00:00:00

  • Pharmacokinetic study of borneol and menthol in rats after oral administration of qingyan drop pills.

    abstract::Both borneol and menthol are bioactive substances derived from Chinese herbal medicines. In order to understand the pharmacokinetics of borneol and menthol in Qingyan drop pills, a rapid, sensitive, and simple gas chromatographic (GC) method with flame ionization detection (FID) was developed for the simultaneous dete...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Xu X,Li Y,Hou J,Zhang S,Xu Y,Wang Y,Zhang Y,Liu C,He X

    更新日期:2011-09-01 00:00:00

  • Halimium halimifolium: From the Chemical and Functional Characterization to a Nutraceutical Ingredient Design.

    abstract::Halimium halimifolium (Hh) is a shrub used in Algerian folk medicine to treat gastrointestinal pain. An UHPLC-PDA-ESI/MSn method was developed to identify the metabolic profile of the traditionally used infusion (Hh-A) from the aerial parts. The structures of flavanols were confirmed by NMR analysis after the isolatio...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Kerbab K,Sansone F,Zaiter L,Esposito T,Celano R,Franceschelli S,Pecoraro M,Benayache F,Rastrelli L,Picerno P,Aquino RP,Mencherini T

    更新日期:2019-08-01 00:00:00

  • LC-MS-Based Quality Assessment of a Traditional Chinese Medicine YANG XIN Formulation.

    abstract::YANG XIN is a traditional Chinese medicine formulation used for nervous fatigue and consists of a proprietary blend of concentrated extracts from 18 plant ingredients. The 18 constituent plant ingredients, YANG XIN capsules, and formulations 2014-005_1 A and 1B were extracted by consecutive 24-hour macerations with di...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Almalki AJ,Zaher A,Simithy J,Keller WJ,Tripp M,Calderón AI

    更新日期:2016-08-01 00:00:00

  • Berberine inhibits cell growth and mediates caspase-independent cell death in human pancreatic cancer cells.

    abstract::Pancreatic cancer is one of the most aggressive human malignancies with an increasing incidence worldwide. In addition to the poor survival rates, combinations using gemcitabine as a backbone have failed to show any benefit beyond monotherapy. These facts underscore an urgent need for novel therapeutic options and mot...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Pinto-Garcia L,Efferth T,Torres A,Hoheisel JD,Youns M

    更新日期:2010-08-01 00:00:00

  • Anti-hepatitic activity of ginesenoside ro1.

    abstract::Ginsenoside Ro, an oleanane-type saponin has been screened for activity in experimental models of acute and chronic hepatitis. Ginsenoside Ro (50 and 200 mg/kg, P.O.) inhibited the increase of serum glutamic oxaloacetic transaminase (s-GOT) and serum glutamic pyruvic transaminase (s-GPT) levels in D-galactosamine (Gal...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Matsuda H,Samukawa K,Kubo M

    更新日期:1991-12-01 00:00:00

  • Inhibition of activator protein 1 activity by berberine in human hepatoma cells.

    abstract::Activator protein 1 (AP-1) is a transcription factor which plays a critical role in inflammation and carcinogenesis. The present study was conducted to investigate the effect of berberine, an isoquinoline alkaloid present in plants of the genera Berberis and Coptis, on the activity of AP-1 using a reporter gene assay ...

    journal_title:Planta medica

    pub_type: 信件


    authors: Fukuda K,Hibiya Y,Mutoh M,Koshiji M,Akao S,Fujiwara H

    更新日期:1999-05-01 00:00:00

  • Comparison of the inhibitory effects of monomeric, dimeric, and trimeric procyanidins on the biochemical markers of skin tumor promotion in mouse epidermis in vivo.

    abstract::Several procyanidin dimers and an epicatechin trimer purified from Douglas fir bark tannins were compared with their monomer components (+)-catechin and (-)-epicatechin for their abilities to inhibit the biochemical effects of the potent tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) in mouse epidermis in v...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Gali HU,Perchellet EM,Gao XM,Karchesy JJ,Perchellet JP

    更新日期:1994-06-01 00:00:00

  • Relaxant effects of quercetin methyl ether derivatives in isolated guinea pig trachea and their structure-activity relationships.

    abstract::In the present study, we attempted to compare quercetin methyl ethers and to look for the structure-activity relationships, which may be helpful for synthesizing more active compounds for the treatment of asthma. Four present and two previously studied quercetin methyl ethers concentration-dependently relaxed histamin...

    journal_title:Planta medica

    pub_type: 信件


    authors: Ko WC,Kuo SW,Sheu JR,Lin CH,Tzeng SH,Chen CM

    更新日期:1999-04-01 00:00:00

  • Polyclonal antibody against a complement-activating pectin from the roots of Angelica acutiloba.

    abstract::Anti-sera against a complement-activating pectin (AR-2IIb), which was purified from the roots of Angelica acutiloba Kitagawa, were obtained by immunization of rabbits, and a polyclonal anti-AR-2IIb antibody of the IgG class was purified by affinity chromatography on AR-2IIb-immobilized Sepharose and Protein G-Sepharos...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Wang NL,Kiyohara H,Matsumoto T,Otsuka H,Hirano M,Yamada H

    更新日期:1994-10-01 00:00:00

  • From ill-defined extracts to the immunomodulatory lectin: will there be a reason for oncological application of mistletoe?

    abstract::There is an obvious discrepancy between the popularity of mistletoe extracts and their classification as a non-conventional treatment modality with unproven efficacy in oncology. The commercial preparations suffer from several major drawbacks: lack of precise declarations for the molecular characteristics and the conc...

    journal_title:Planta medica

    pub_type: 杂志文章,评审


    authors: Gabius HJ,Gabius S,Joshi SS,Koch B,Schroeder M,Manzke WM,Westerhausen M

    更新日期:1994-02-01 00:00:00

  • Antifungal sesquiterpenes from Cedrus deodara.

    abstract::Two new sesquiterpenes, (E)-(2S,3S,6R)-atlantone-2,3-diol (1) and (E)-(2S,3S,6S)-atlantone-2,3,6-triol (2), along with two known sesquiterpenes, atlantolone (3) and (E)-α-atlantone (4), were isolated from Cedrus deodara Loud. The structures of the new compounds were elucidated on the basis of UV, IR, NMR, HR‑ESI-QTOF‑...

    journal_title:Planta medica

    pub_type: 信件


    authors: Chaudhary A,Sood S,Kaur P,Kumar N,Thakur A,Gulati A,Singh B

    更新日期:2012-01-01 00:00:00

  • Determination of naphthazarin derivatives in endemic Turkish Alkanna species by reversed phase high performance liquid chromatography.

    abstract::The quantification of free and total alkannins in 13 endemic Anatolian Alkanna species is described for the first time. Extraction of the samples was performed by sonication with hexane, followed by hydrolysis in 1 N NaOH. For analysis, a new HPLC method, utilizing reversed phase material (Synergi Max RP), was develop...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Pekin G,Ganzera M,Senol S,Bedir E,Korkmaz KS,Stuppner H

    更新日期:2007-03-01 00:00:00

  • Marine natural products research: current directions and future potential.

    abstract::Natural products research is increasingly turning to marine animals, plants, and microbes as source organisms. Several marine natural products are currently in preclinical and clinical evaluation, others show promising biological activities in in vitro and in vivo assays. Investigations of biological and chemical ecol...

    journal_title:Planta medica

    pub_type: 杂志文章,评审


    authors: König GM,Wright AD

    更新日期:1996-06-01 00:00:00

  • New naphthalenyl glycosides from the roots of Juglans mandshurica.

    abstract::Three new glycosides, 1,4,8-trihydroxynaphthalene-1-O-D-glucopyranosyl-(1-->6)-beta-D- xylopyranoside (1), 1,4,8-trihydroxynaphthalene-1-O-beta-D-glucopyranosyl-(1-->6)-alph a-L- arabinopyranoside (2), 1-hydroxy-4-methoxynaphthalene-1-O-beta-D-glucopyranosyl-(1-->6)- alpha-L-rhamnopyranoside (3), were isolated from th...

    journal_title:Planta medica

    pub_type: 信件


    authors: Lee SW,Lee KS,Son JK

    更新日期:2000-03-01 00:00:00

  • Encelin: a fungal growth inhibitor.

    abstract::A known sesquiterpene lactone, encelin, isolated from the Mexican species Montanoa speciosa (Compositae), was assayed for biocidal activity on fungal cells of Mucor rouxii. The results indicate that encelin has a determining action on growth and the morphogenetic process of fungal cells. ...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Sabanero M,Quijano L,Rios T,Trejo R

    更新日期:1995-04-01 00:00:00

  • Standardization and in vivo antioxidant activity of ethanol extracts of fruit and leaf of Piper sarmentosum.

    abstract::The present study aimed to investigate standardized ethanol extracts of fruit and leaves of Piper sarmentosum for their in vivo antioxidant activity in rats using a CCl (4)-induced oxidative stress model. The standardization was based on the quantification of the markers pellitorine, sarmentine and sarmentosine by hig...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Hussain K,Ismail Z,Sadikun A,Ibrahim P

    更新日期:2010-03-01 00:00:00

  • Inhibition of rat adjuvant-induced arthritis by ginkgetin, a biflavone from ginkgo biloba leaves.

    abstract::Ginkgetin, a biflavone isolated from Ginkgo biloba leaves, was previously reported as an inhibitor of group II phospholipase A2. In this study, ginkgetin was evaluated for in vivo antiarthritic and analgesic activities. Ginkgetin (10-20 mg/kg/day) strongly reduced arthritic inflammation in an animal model of rat adjuv...

    journal_title:Planta medica

    pub_type: 信件


    authors: Kim HK,Son KH,Chang HW,Kang SS,Kim HP

    更新日期:1999-06-01 00:00:00

  • Inhibition of HCV replicon cell growth by 2-arylbenzofuran derivatives isolated from Mori Cortex Radicis.

    abstract::Medicinal herbs are increasingly used in the search for safe and efficient drug candidates for hepatitis C virus infection. In this study, we have investigated the anti-HCV effect of compounds from Mori Cortex Radicis. During a screening for extracts with anti-HCV affinity from medicinal plants (173 species), the meth...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Lee HY,Yum JH,Rho YK,Oh SJ,Choi HS,Chang HB,Choi DH,Leem MJ,Choi EJ,Ryu JM,Hwang SB

    更新日期:2007-11-01 00:00:00

  • Chemical properties and anti-complementary activities of polysaccharide fractions from roots and leaves of Panax ginseng.

    abstract::Water-soluble and alkaline-soluble polysaccharide fractions, prepared from roots and leaves of PANAX GINSENG C.A. Meyer, were fractionated into strongly acidic, weakly acidic, and neutral polysaccharide fractions by cetyltrimethyammonium bromide, respectively. The roots contained a larger amount of crude polysaccharid...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Gao QP,Kiyohara H,Cyong JC,Yamada H

    更新日期:1989-02-01 00:00:00

  • Tetrandrine selectively protects against amyloid-beta protein - but not against MPTP-induced cytotoxicity in SK-N-SH neuroblastoma cells.

    abstract::The evidence for loss of Ca2+ homeostasis due to neuronal degeneration is considerable and rapidly increasing. In this study, we try to evaluate the protective effect of tetrandrine (TET), an alkaloid isolated from the Chinese medicinal herb Radix Stephania tetrandrae S., on amyloid-beta protein (Abeta) and 1-methyl-4...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Chiou WF,Lee WS,Yeh PH

    更新日期:2006-11-01 00:00:00

  • Labdane diterpenoids and shikimic acid derivatives from Araucaria cunninghamii.

    abstract::Four new (1-4) and two known compounds (5, 6) were isolated from the aerial parts of Araucaria cunninghamii. By analysis of UV, IR, MS, and NMR, the new compounds were identified as ENT-19-(Z)-coumaroyloxylabda-8(17),13(16),14-triene (1), ENT-19-( E)-coumaroyloxylabda-8(17),13(16),14-triene (2), shikimic acid N-butyl ...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Chen J,Chen JJ,Yang LQ,Hua L,Gao K

    更新日期:2011-03-01 00:00:00

  • Antiproliferative effects on tumour cells and promotion of keratinocyte wound healing by different lichen compounds.

    abstract::Five compounds representative of major structural classes of lichen polyketides, VIZ. (+)-usnic (1), salazinic (2), vulpinic (3), gyrophoric (4), and evernic acids (5), were investigated for their ability to affect cell proliferation or wound healing, two functional targets of relevance for research on cancer or tissu...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Burlando B,Ranzato E,Volante A,Appendino G,Pollastro F,Verotta L

    更新日期:2009-05-01 00:00:00

  • Formation of Digitalis lanata Clone Lines by Shoot Tip Culture.

    abstract::Propagation of DIGITALIS LANATA by shoot tip culture made possible (a) the rapid multiplication of elite plants with the formation of plant clones and (b) the long-term cultivation of these plants which under normal growth conditions would die at the end of the second vegetation period. Optimum conditions were establi...

    journal_title:Planta medica

    pub_type: 杂志文章


    authors: Diettrich B,Mertinat H,Luckner M

    更新日期:1990-02-01 00:00:00

  • Composition and antimicrobial activity of the essential oil of Salvia ringens.

    abstract::The essential oils of Salvia ringens (samples A and B), were analyzed by means of GC and GC/MS. From the seventy-five identified constituents representing 99.82 and 99.86% of the oils, 1,8-cineole and alpha-pinene were the major components. Furthermore, sample B exhibited a very interesting antimicrobial profile after...

    journal_title:Planta medica

    pub_type: 信件


    authors: Tzakou O,Pitarokili D,Chinou IB,Harvala C

    更新日期:2001-02-01 00:00:00