Abstract:
:Data are presented on the urinary corticosteroid metabolic profile of the mouse strain 129/svJ. Through the use of GC/MS we have characterized, or tentatively identified corticosterone (Kendall's compound B) metabolites of both the 11beta-hydroxy and 11-carbonyl (compound A) series in urine. Full mass spectra of the methyloxime-trimethylether derivatives of 15 metabolites are included in the paper as an aid to other researchers in the field. Metabolites ranged in polarity from tetrahydrocorticosterone (THB) to dihydroxy-corticosterone with dominance of highly polar steroids. We found that prior to excretion corticosterone can undergo oxidation at position 11beta, reduction at position 20 and A-ring reduction. Metabolites retaining the 3-oxo-4-ene structure can be hydroxylated at position 6beta- as well as at an unidentified position, probably 16alpha-. Saturated steroids can be hydroxylated at positions 1beta-, 6alpha-, 15alpha- and 16alpha. A pair of hydroxy-20-dihydro-corticosterone metabolites (OH-DHB) were the most important excretory products accounting for about 40% of the total. One metabolite of this type was identified as 6beta-hydroxy-DHB; the other, of similar quantitative importance was probably 16alpha-hydroxy-DHB. The ratio of metabolites of corticosterone (B) to those of 11-dehydro-corticosterone (A) was greater than 9:1, considerably higher than that for the equivalent "human" ratio of 1:1 for cortisol to cortisone metabolites. Results from this study allowed the evaluation of 11beta-hydroxysteroid dehydrogenase (11beta-HSD) activity in mice with deleted glucose-6-phosphate transporter (G6PT). These mice had attenuated back-conversion of A to B resulting in an increased ratio of A-metabolites to B-metabolites [Walker EA, Ahmed A, Lavery GG, Tomlinson JW, Kim SY, Cooper MS, Stewart PM, 11beta-Hydroxysteroid dehydrogenase type 1 regulation by intracellular glucose-6-phosphate, provides evidence for a novel link between glucose metabolism and HPA axis function. J Biol Chem 2007;282:27030-6]. We believe this study is currently the most comprehensive on the urinary steroid metabolic profile of the mouse. Quantitatively less steroid is excreted in urine than in feces by this species but urine analysis is more straightforward and the hepatic metabolites are less subject to microbial degradation than if feces was analyzed.
journal_name
Steroidsjournal_title
Steroidsauthors
Shackleton CH,Hughes BA,Lavery GG,Walker EA,Stewart PMdoi
10.1016/j.steroids.2008.04.004subject
Has Abstractpub_date
2008-10-01 00:00:00pages
1066-76issue
11eissn
0039-128Xissn
1878-5867pii
S0039-128X(08)00114-1journal_volume
73pub_type
杂志文章相关文献
STEROIDS文献大全abstract::The synthesis of delta 7,9(11)-lanostadiene derivatives functionalized at C(32) starting from 3 beta-acetoxy-7 alpha,32-epoxylanostan-11-one has been presented. The delta 7,9(11) moiety was efficiently introduced in three steps in 71% yield by the regioselective abstraction of allylic 8 beta hydrogen. The formyl group...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(97)00075-5
更新日期:1997-11-01 00:00:00
abstract::The aim of the present work was to analyze the effect of dehydroepiandrosterone (DHEA) on several metabolic risk factors, including cardiovascular health and insulin resistance, in aged rats submitted to a high-fat diet. For that, weaned rats were fed on a high-fat diet until 20 months of age. In the last 13 weeks of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.04.011
更新日期:2008-10-01 00:00:00
abstract::Bile acid salts are biosurfactants which form mixed micelles with phospholipids in vertebrates. These mixed micelles are suitable for solubilisation of cholesterol. For therapeutic purposes some bile acid salts as sodium ursocholate are used. However, bile acid anions possess low capacity for solubilisation of cholest...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.04.018
更新日期:2014-08-01 00:00:00
abstract::A partial synthesis of the title compound, 4'-(dimethylamino)-17 beta-hydroxy-17 alpha-(1-propynyl)benzo[12,12a]-11 alpha,18-cyclo-12a,12b- dihomo-13 alpha-estr-4-en-3-one 1, is reported. The key step in this synthesis represents an intramolecular alkenylaryl radical cyclization. Treatment of 18-[bromo-5-(dimethylamin...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90026-4
更新日期:1994-03-01 00:00:00
abstract::Studies were carried out on the effect of oxygen tension on progesterone (P) accumulation in rat granulosa cell cultures. At 1-2% oxygen, basal, luteinizing hormone (LH)-stimulated, and follicle stimulating hormone (FSH)-stimulated P accumulations were 20, 18, and 11%, respectively, of P levels at 20% oxygen. Basal P ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90047-0
更新日期:1989-11-01 00:00:00
abstract::The formation of a horseradish peroxidase-testosterone conjugate for the enzyme-linked immunoassay of testosterone was investigated, using tritiated testosterone to follow the reaction. The formation of testosterone-3-(carboxymethyl) oxime-peroxidase by the mixed anhydride method was found to give a conjugate of high ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90021-6
更新日期:1977-05-01 00:00:00
abstract::We synthesized 22-fluorovitamin D3 from (22S) cholest-5-ene-3 beta, 22-diol-3 beta-acetate 2. Compound 2 was treated with diethylaminosulfur trifluoride to give 22-fluorocholest-5-en-3 beta-acetate 3 and (E) 22-dehydrocholest-5-en-3 beta-acetate. Compound 3 was treated with N-bromosuccinimide to give a mixture of the ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(86)90044-9
更新日期:1986-07-01 00:00:00
abstract::Cortisol 21-amine (21-amino-11beta,17-dihydroxy-4-pregnene-3,20-dione) was prepared and an enzyme immunoassay for cortisol in serum was established using cortisol 21-amine conjugated with alkaline phosphatase. The minimal amount of cortisol detected was 1ng/tube and the measurable range was from 1 to 80 microgram/d1, ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90106-x
更新日期:1978-07-01 00:00:00
abstract::Preimplantation rabbit embryos were incubated with pregnenolone and dehydroisoandrosterone under conditions which gave formazan precipitation by the histochemical technique. The metabolic fate of the labeled steroids was assessed simultaneously. There was no concomitant transformation of pregnenolone to progesterone a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80012-8
更新日期:1981-02-01 00:00:00
abstract::The full assignments of the (1)H and (13)C NMR signals of steroids bearing the 16beta,23:23,26-diepoxy side chain are provided. Differentiation of the diasterotopic H-26 pair was achieved with the aid of NOESY experiments. The main substituent and steric effects associated with this moiety and their influence on the c...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.01.027
更新日期:2008-07-01 00:00:00
abstract::Efforts to develop a novel class of nonsteroidal aromatase inhibitors began with the discovery that the infertility in male rats exposed to high levels of the agricultural fungicide, fenarimol (alpha-(2-chlorophenyl)-alpha-(4-chlorophenyl)-5-pyrimidine-methanol), was attributable to the inhibition of aromatase activit...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90072-7
更新日期:1987-07-01 00:00:00
abstract::CGS 16949A inhibited the conversion of [4-14C]androstenedione (A) to [4-14C]estrone by human placental microsomes in a competitive manner (Ki = 1.6 nM). Aminoglutethimide, also a competitive inhibitor, had a Ki = 0.7 microM in this assay system. The Km for the aromatization of A was 0.11 microM. Using ovarian microsom...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90068-5
更新日期:1987-07-01 00:00:00
abstract::Gypensapogenin H (GH) is a novel dammarane-type triterpenes obtained from hydrolyzate of total saponins from Gynostemma pentaphyllum and its anti-tumor activity has been studied in previous work. In this study, we report the effects of this compound on human prostate cancer cells (DU145 and 22RV-1). It significantly i...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.10.014
更新日期:2015-12-01 00:00:00
abstract::The purpose of this study was to determine the short-term effects of progesterone action on estrogen receptor (Re) levels in the rat uterus. Ovariectomized, adrenalectomized rats were maintained on subcutaneous Silastic implants containing crystalline estradiol. Progesterone treatment with serum estradiol maintenance ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90047-7
更新日期:1981-04-01 00:00:00
abstract:BACKGROUND:Once a septic condition is progressing, administration of steroids in the pro-inflammatory phase of septic shock ought to yield maximal effect on the subsequent, devastating inflammatory response. Recently, a retrospective study showed that early initiation of corticosteroid therapy improved survival in sept...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.06.001
更新日期:2012-09-01 00:00:00
abstract::The synthesis of the esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one) with three bile acids and of the cholesteryl carbonate of norethisterone are described. :This article reports the synthesis of the esters of norethisterone (17 alpha-ethynl-17beta-hydroxyestr-4-en-3-one) with 3 bile acids ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90103-4
更新日期:1983-03-01 00:00:00
abstract::Oleoyl-estrone (OE) induces a marked loss of body fat in rats by maintaining energy expenditure, body protein and blood glucose despite decreasing food intake. OE increases glucocorticoids, but they arrest OE lipid-mobilization. We studied here whether OE induces a direct effect on adrenal glands function as part of t...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2009.09.009
更新日期:2010-01-01 00:00:00
abstract::The mechanistic aspects of the alkali-catalyzed rearrangement of 16alpha-hydroxy-17-keto steroid 1 to 17beta-hydroxy-16-keto steroid 2 are elucidated by use of (18)O- and deuterium-labeling experiments. The (18)O-labeling experiments refute the gem-hydration-quasi-diaxial dehydration mechanism for the rearrangement pr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.03.001
更新日期:2008-09-01 00:00:00
abstract::Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90001-x
更新日期:1980-09-01 00:00:00
abstract::The free sterol mixture of the sponge Psammaplysilla purpurea was shown to contain aplysterol as the major constituent. In addition to other sterols such as 5,7-cholestadien-3beta-ol, cholesterol, 5alpha-cholestan-3beta-ol, 24epsilon-methylcholesta-5,22-dien-3beta-ol, 24epsilon--methylcholesterol, 24epsilon-ethylchole...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80046-4
更新日期:1978-06-01 00:00:00
abstract::Synthesis, biochemical and biological testing of the first carborane derivatives of estrogens are described. Estrone 3-carboranylmethyl ether was synthesized in two steps from estrone. Reduction of estrone 3-carboranylmethyl ether with sodium borohydride provided estradiol-17 beta 3-carboranylmethyl ether. Enzyme kine...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80020-7
更新日期:1981-02-01 00:00:00
abstract::This paper revealed the expression pattern of ERα in the ovoviviparous teleost, Sebastes schlegeli. In this paper, we isolated the cDNA encoding for estrogen receptor alpha of black rockfish (S. schlegeli) from its ovary, named as black rockfish ERα (brfERα). The cDNA sequence of brfERα consists of 2972bp with an open...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.09.014
更新日期:2011-12-20 00:00:00
abstract::Dehydroepiandrosterone (DHEA) fatty acyl esters once incorporated in high density lipoprotein (HDL) induce a stronger vasodilatory response in rat mesenteric arteries ex vivo compared to native HDL. We studied the role of HDL receptor, scavenger receptor class B, type 1 (SR-B1), as well as estrogen and androgen recept...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.12.007
更新日期:2011-03-01 00:00:00
abstract::Sleep apnea is a disorder, which increasingly affects people worldwide. Whether the associated hypoxic events during sleep are central or obstructive in origin, the end result is excessive daytime sleepiness and an increased risk for several comorbidities, such as cardiovascular and neurodegenerative disorders. Sleep ...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2017.12.006
更新日期:2018-05-01 00:00:00
abstract::Explants prepared from the neocortex and the fetal zone of the human fetal adrenal (gestational age 13 to 18weeks) were maintained under conditions of organ culture for 7 to 9 days during which time they were exposed to hACTH and various related peptides. Corticotrophic activity was monitored by the daily release of d...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90037-5
更新日期:1978-04-01 00:00:00
abstract::Testicular homogenates of tamoxifen-treated rats were incubated with labeled steroid precursors (progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione or testosterone) in order to study the effect of tamoxifen on testicular steroidogenesis. The results indicate that a 9 day treatment with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90062-7
更新日期:1989-06-01 00:00:00
abstract::Circulating levels (mean +/- SD) of estrone sulfate (E1S), estrone (E1) and estradiol-17 beta (E2) were measured in normal and cirrhotic postmenopausal women matched for body weight and age. In cirrhotic postmenopausal women, the E1S concentrations (201 +/- 46 pg/ml), while both E1 and E2 levels showed an increase (46...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90022-3
更新日期:1983-05-01 00:00:00
abstract::Based on histological criteria, Kingsley and Bogdanove (3) reported that the benzoate ester of 17beta-hydroxy-5alpha-androstan-3-one (5alpha-DHT), unlike testosterone propionate, is unable to induce vaginal mucification when given subcutaneously to rats. In contrats, Kennedy (4) found in estrogen-pretreated rats that ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90061-1
更新日期:1976-03-01 00:00:00
abstract::Carbenoxolone potentiates the mineralocorticoid activity of endogenous glucocorticoid hormones by inhibiting the enzyme 11 beta-hydroxysteroid dehydrogenase, which converts cortisol and corticosterone to inactive 11-oxo-derivatives. We addressed the question of whether glucocorticoid activity is also affected by carbe...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(96)00252-8
更新日期:1997-04-01 00:00:00
abstract::Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(82)90084-8
更新日期:1982-02-01 00:00:00