Abstract:
:A 30-residue peptide corresponding to the amino acid sequence of steroidogenesis activator peptide (SAP) from rat Leydig tumor cells has been synthesized by the solid-phase method using Boc protection. SAP is the putative cycloheximide-sensitive, cAMP-regulated mediator of ACTH-stimulated conversion of cholesterol to pregnenolone in adrenal cortex. N alpha-acetyl-SAP(11-30), an NH2-terminally truncated steroidogenesis activator peptide analog that is missing the most hydrophobic portion of SAP, was also prepared. In addition to these two peptides, N alpha-acetyl-(Cys0)SAP was synthesized in both non-radiolabeled and tritiated forms for coupling to carrier proteins for use as an immunogen to raise anti-SAP antibodies. Chain elongation during synthesis of SAP on PAM resin proceeded with an average coupling yield of 99.3% as determined by quantitative ninhydrin tests. After HF cleavage at -7 degrees, the crude products were purified by semi-preparative HPLC. Peptides were analyzed by analytical HPLC, amino acid analysis, tryptic peptide mapping, and by UV and CD spectroscopy. As determined by CD spectra, SAP showed little evidence of preferred structure either in aqueous solution in the presence of divalent cations or in micelles of reduced Triton X-100 in the absence or presence of either cholesterol or phosphatidylcholine. SAP, in conjunction with GTP, enhanced side chain cleavage activity in isolated adrenal mitochondria.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Glass DB,Robertson DG,Xu TS,Bowman EP,Lambeth JDdoi
10.1080/07435809109027204subject
Has Abstractpub_date
1991-01-01 00:00:00pages
307-26issue
1-2eissn
0743-5800issn
1532-4206journal_volume
17pub_type
杂志文章abstract:PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1141936
更新日期:2016-11-01 00:00:00
abstract::The liver is known to be sensitive to dietary challenge and to reduction in its functional mass. To investigate the influence of the vitamin D endocrine system on the hepatic growth response, liver DNA synthesis (S phase of cell cycle) was primed by dietary manipulation (high carbohydrate/protein-free diet x 3 d follo...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809109106818
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:To determine whether daily oral micronized progesterone affects bone turnover, as estimated by serum and urine biochemical markers, in postmenopausal women on long-term estrogen replacement therapy (ERT). METHODS:We recruited 14 women aged 65 or older to participate in a 9-week trial with micronized progestero...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-120022294
更新日期:2003-05-01 00:00:00
abstract:AIM:To evaluate, retrospectively, the frequency of antithyroid antibodies (ATA) in patients with type 1 diabetes (T1D). MATERIALS AND METHODS:Antithyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and antithyroid-stimulating hormone receptor antibodies (TSHR-Ab) were determined by enzyme-linked...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.639318
更新日期:2012-01-01 00:00:00
abstract::Pertussis toxin (PT) and cholera toxin (CT) have been shown to modulate lactogenic hormone-stimulated Nb2 cell mitogenesis, a lactogen-dependent cell line. As both toxins have been shown to alter guanylate cyclase activity in other cell systems, cyclic guanosine monophosphate (cGMP) analogs, 8-bromo or dibutyryl cGMP,...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809209035926
更新日期:1992-01-01 00:00:00
abstract::The secretory activity of the adrenal gland is mainly regulated by peptidergic hormones (ACTH, angiotensin II) and ions. However, there is now increasing evidence that local factors, including neuropeptides and neurotransmitters, can also participate in the control of adrenocortical cells. In particular, serotonin (5-...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032670
更新日期:1998-08-01 00:00:00
abstract:PURPOSE:Monthly regimen of minodronate for osteoporosis more than two years has not been reported yet. The aim of this study is to elucidate the effect of monthly minodronate (M-MIN) on bone mineral density (BMD) and serum pentosidine (Pen) during 27 months. MATERIALS AND METHODS:The study consisted of 52 newly treate...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1292527
更新日期:2017-08-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) levels were found to be associated with obesity, indicating that adipocytes might influence PAI-1 plasma levels. In addition, the 4G/5G promoter polymorphism of the PAI-1 gene possibly modulates PAI-1 gene transcription and, as a consequence, PAI-1 plasma levels. Metabolic par...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200035728
更新日期:2004-08-01 00:00:00
abstract::The B vitamin nicotinamide (NIC), commonly known as niacin, is currently in trial as a potential means of preventing Type 1 diabetes in first-degree relatives of affected individuals. Sodium butyrate (BUT) a common dietary micronutrient has also been reported to have beneficial effects on the differentiation and funct...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120028485
更新日期:2004-02-01 00:00:00
abstract:INTRODUCTION:Adenosine deaminase (ADA), purine nucleoside phosphorylase (PNPase), S-adenosylhomocysteine hydrolase (SAHH), 5'-nucleotidase (5N), and deoxycytidine kinase (dCK) are involved in purine salvage metabolism. Changes of the activities of the above enzymes have been observed in blood cells in patients with imm...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120015059
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVES:The diagnosis and management of osteoporosis and osteoporotic fractures are challenging in rural and underdeveloped areas of China because medical resources are inaccessible; thus, a simple and accurate method is essential for the detection of vertebral fractures. We aimed to examine the relationship between...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2020.1827263
更新日期:2021-02-01 00:00:00
abstract::In order to clarify the roles of cholinergic and serotonergic neurotransmission in the mediation of exercise-induced growth hormone (GH) release, normal young volunteers of both sexes were studied. Exercise was for 20 minutes at 800 kpm for the men and 500 kpm for the women. Pretreatment with 0.4 mg atropine 1 hour pr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035433
更新日期:1986-01-01 00:00:00
abstract::There are striking similarities between Cushing's syndrome and the 'metabolic syndrome X' since both are characterised by hypertension, insulin resistance, glucose intolerance, hyperlipidaemia, and central obesity. The possibility that cortisol contributes to the associations between multiple risk factors for cardiova...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435809609043765
更新日期:1996-11-01 00:00:00
abstract:AIM:To determine whether hyperuricemia is associated with the early phase and late phase of insulin release in apparently healthy non-obese subjects with normal glucose tolerance. MATERIALS AND METHODS:A population-based cross-sectional study was performed. Apparently healthy individuals aged 20 to 65 years were eligi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1155597
更新日期:2017-02-01 00:00:00
abstract::Hashimoto's thyroiditis (HT) is one of the most common organ-specific autoimmune diseases. Increasing evidence indicates that HT may be characterized by an imbalance in the helper T cell subsets Th1 and Th2. Traditional Chinese Medicine (TCM) considers HT as a chronic exhaustion disease, leading to deficiency of qi. I...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1066800
更新日期:2016-01-01 00:00:00
abstract::Combined partial deficiency of 17alpha-hydroxylase and 21-hydroxylase activities was first described in 1985; however the genes for P450c17 and P450c21 in these patients lack mutations. In 1986 we postulated that this disorder might be due to mutations in P450 oxidoreductase (POR), the flavoprotein that donates electr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044134
更新日期:2004-11-01 00:00:00
abstract::Congenital adrenal hyperplasia due to 21-hydroxylase (21-OH) deficiency is HLA-linked. The haplotype HLA-(A3);Bw47;DR7 is strongly associated with 21-OH deficiency and always carries a null allele at the complement C4A (Rodgers) locus. It seemed likely that this haplotype carries a deletion encompassing both the C4A a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808409036505
更新日期:1984-01-01 00:00:00
abstract::In order to investigate the regulation of connexin distribution in rapidly growing cells, we evaluated the effects of dibutyryl cyclic adenosine monophosphate (DbcAMP) treatment on the cellular location of endogenous connexin 43 (Cx43) in an adrenal tumor cell population. Polyclonal antibodies to Cx43 and a Cx43 green...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016983
更新日期:2002-11-01 00:00:00
abstract::TRH is negatively regulated by T3 both in the hypothalamic paraventricular nucleus and transient transfection models. Mutations in hTR beta 1 genes are associated with the syndrome of generalized resistance to thyroid hormone. To investigate potential effects of mutant TRs on T3 regulation of the hTRH gene, transient ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031859
更新日期:1997-11-01 00:00:00
abstract::Several lines of evidence have suggested that the nuclear receptor Steroidogenic Factor-1 (SF-1 or Ad4BP) may be directly involved in the cAMP-dependent regulation of steroid hydroxylase genes in adrenocortical cells. In the bovine CYP17 gene, which encodes the cytochrome P450 17alpha-hydroxylase, an SF-1 site is pres...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032638
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND:Small dense low-density lipoprotein (sd-LDL) has been demonstrated to be associated with cardiovascular diseases (CVD). The proposed atherogenic properties of hypoadiponectinemia might be mediated through increased sd-LDL. In this study, the associations of sd-LDL with cardiovascular and other complications ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2014.913614
更新日期:2015-01-01 00:00:00
abstract::The question of whether ethanol's (ETOH's) known suppressive effect on serum luteinizing hormone (LH) could be mediated directly at the anterior pituitary level was addressed by examining the effects of ETOH in vitro on release of LH from cultured male rat pituitary cells. The impact of added ethanol concentrations ra...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808909042748
更新日期:1989-01-01 00:00:00
abstract::We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107168
更新日期:2001-02-01 00:00:00
abstract::The cdc2 kinase family is known to be one of the important factors for cell proliferation in both yeast and mammalian cells. By using polyclonal antibodies against PSTAIRE region of cdc2HS, we studied the amount of cdc2-related kinases in the rat thyroid cell line, FRTL-5, during the cell cycle. The immunoreactive pro...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809409030404
更新日期:1994-05-01 00:00:00
abstract:UNLABELLED:It remains difficult to pre-operatively differentiate follicular carcinoma and adenoma even under intraoperative investigations including histopathologic diagnosis of frozen sections. METHODS:We retrospectively compared dual phase thallium (Tl-201) thyroid scan and fine-needle aspiration (FNA) cytology to d...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120025036
更新日期:2003-08-01 00:00:00
abstract::The effect of crowding on the pituitary-gonadal axis was studied in adult male rats. Crowding reduced body weight gain, did not alter relative adrenal weight and increased relative testis weight. Neither basal levels of corticosterone nor its response to acute stress were altered by crowding. Likewise, LH secretion wa...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035431
更新日期:1986-01-01 00:00:00
abstract:INTRODUCTION:Charcoal- or resin-stripping of fetal bovine serum (FBS) or bovine calf serum (BCS) intended for supplementation of cell culture media is widely practiced to remove a variety of endogenous compounds, including steroid, peptide, and thyroid hormones. The possibility that stripping removes other biologically...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800903204082
更新日期:2009-01-01 00:00:00
abstract::Metabolic syndrome was first proposed in 1988 and has been recognized as a powerful predictor for cardiovascular disease and diabetes. At the same time, white blood cell count (WBCC) was proposed to have significant correlation with metabolic syndrome (MS). In this study, we attempted to investigate the relationship b...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500229151
更新日期:2005-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate the expression of the glucose transporters GLUT1 and GLUT3 in papillary thyroid carcinomas (PTCs) and to elucidate their relationship with the BRAF V600E mutation and F-18 FDG uptake. MATERIALS AND METHODS:We retrospectively analyzed data of 52 PTC patients (41 women a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1066803
更新日期:2016-01-01 00:00:00