Abstract:
:Several lines of evidence have suggested that the nuclear receptor Steroidogenic Factor-1 (SF-1 or Ad4BP) may be directly involved in the cAMP-dependent regulation of steroid hydroxylase genes in adrenocortical cells. In the bovine CYP17 gene, which encodes the cytochrome P450 17alpha-hydroxylase, an SF-1 site is present within cAMP-responsive sequence 2 (CRS2) and mutations which interfere with SF-1 binding correlate with decreases in cAMP-stimulated transcription of a linked reporter gene. In order to determine whether the cAMP response relies on structures within SF-1 itself, mutations and deletions were introduced. We demonstrate that even a single point mutation (E454A) in the transactivating AF-2 domain drastically reduces the ability of SF-1 to mediate cAMP-dependent transcription. Furthermore, the mutation results in a protein which acts in a dominant negative fashion with respect to cAMP-dependent regulation of the bovine CYP17 gene. Finally, we demonstrate that the coactivators CBP and SRC-1 are limiting with respect to cAMP-induced CRS2-dependent transcription in Y1 adrenocortical tumor cells, suggesting that part of the action of cAMP may be to influence the interaction of SF-1 with other cofactors via the AF-2 domain.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Lund J,Jacob A,Aesöy R,Yri OE,Mellgren Gdoi
10.3109/07435809809032638subject
Has Abstractpub_date
1998-08-01 00:00:00pages
497-504issue
3-4eissn
0743-5800issn
1532-4206journal_volume
24pub_type
杂志文章abstract::Mouse Y1 adrenocortical tumor cells harbor amplified and overexpressed c-Ki-ras gene, displaying relatively high constitutive levels of Ras x GTP. Here we report that Y1 cells also constitutively display high levels of phosphorylated AKT/PKB, that are dependent on Ras x GTP and PI3K. ACTH rapidly causes dephosphorylat...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048616
更新日期:2000-11-01 00:00:00
abstract::Adrenal computed tomography with determination of Hounsfield units has proved to be sensitive and specific in the differential diagnosis of benign vs. malign adrenal lesions. On the other hand, computed tomography may fail in patients with small adrenal masses of less than 1.0 cm. However, especially in patients with ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120017075
更新日期:2002-11-01 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) levels were found to be associated with obesity, indicating that adipocytes might influence PAI-1 plasma levels. In addition, the 4G/5G promoter polymorphism of the PAI-1 gene possibly modulates PAI-1 gene transcription and, as a consequence, PAI-1 plasma levels. Metabolic par...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200035728
更新日期:2004-08-01 00:00:00
abstract::The adrenal cortex is a complex tissue comprising different cell types, including endocrine, stromal, nerve, perivascular, and endothelial cells. The pituitary hormone ACTH controls the coordinated development of the vasculature and the endocrine tissue mass. This suggests that paracrine secretions between steroidogen...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043623
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Ultrasonography is considered useful to distinguish between solid and cystic thyroid nodules and to stratify a nodule's risk of cancer as low, medium, or high. Ultrasound (US) elastography has been applied to study the hardness/elasticity of nodules to differentiate malignant from benign lesions. Elastograph...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.633952
更新日期:2012-01-01 00:00:00
abstract::The enzyme 3beta-hydroxysteroid dehydrogenase plays a crucial role in the steroidogenic process in the adrenal gland. In the present study we tried to characterize its localization and developmental changes in the rat adrenal cortex during the postnatal period, using immunohistochemical methods. The development of the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066126
更新日期:1999-02-01 00:00:00
abstract::Excessive aldosterone secretion in some hypertensive patients may result from abnormal aldosterone synthase (AS) gene regulation in response to changes in dietary sodium intake. We have utilized NCI-H295 cells, which exhibit stable angiotensin-induced aldosterone secretion, for transient transfections with murine AS/h...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030462
更新日期:1995-02-01 00:00:00
abstract::In order to study the role of Platelet Activating Factor (PAF) in the mechanism of parturition and connective tissue metabolism in the pregnant rat uterus, we investigated the influence of the Platelet Activating Factor Receptor Antagonist (BN 52021) on gestation duration and collagen concentration in pregnant rat ute...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809409030414
更新日期:1994-11-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00
abstract::Total collagen and its soluble fraction as well as water content were determined in late pregnancy in the uterine cervix in a population of 36 rats treated with progesterone (n = 10), mifepristone (n = 13) and controls (n = 13). In both treated groups there were no signs of delivery on the 21st day of gestation. Water...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809509030477
更新日期:1995-08-01 00:00:00
abstract::Interleukin-23 receptor (IL-23R) and signal transducer and activator of transcription 3 (STAT3) polymorphisms are common risk factors for a number of T helper (Th) 17-mediated autoimmune diseases. However, the importance of genetic variations in Th17 pathways to thyroid autoimmunity, and particularly Hashimoto's thyro...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.875038
更新日期:2014-01-01 00:00:00
abstract:AIM:To evaluate, retrospectively, the frequency of antithyroid antibodies (ATA) in patients with type 1 diabetes (T1D). MATERIALS AND METHODS:Antithyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and antithyroid-stimulating hormone receptor antibodies (TSHR-Ab) were determined by enzyme-linked...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.639318
更新日期:2012-01-01 00:00:00
abstract::The determination of DNA ploidy and the study of the cell cycle in adrenocortical tumoral cells could help in the distinction between benign and malignant lesions and also in the prediction of the biological behaviour of these tumors. We analysed 32 cases of adrenal tissue (8 normal adrenals--N, 12 benign adenomas--A ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032700
更新日期:1998-08-01 00:00:00
abstract::The liver is known to be sensitive to dietary challenge and to reduction in its functional mass. To investigate the influence of the vitamin D endocrine system on the hepatic growth response, liver DNA synthesis (S phase of cell cycle) was primed by dietary manipulation (high carbohydrate/protein-free diet x 3 d follo...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809109106818
更新日期:1991-01-01 00:00:00
abstract::There are striking similarities between Cushing's syndrome and the 'metabolic syndrome X' since both are characterised by hypertension, insulin resistance, glucose intolerance, hyperlipidaemia, and central obesity. The possibility that cortisol contributes to the associations between multiple risk factors for cardiova...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435809609043765
更新日期:1996-11-01 00:00:00
abstract::The steady state level of most cellular phosphoproteins is dependent on the relative catalytic activities of intracellular protein kinases and phosphatases. In adrenal cortex, ACTH acts through PKA activation and Ser/Tre phosphorylation. Phosphatases involved in this pathway are not completely described, particularly ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032618
更新日期:1998-08-01 00:00:00
abstract::The rat adrenal cortex possesses at least two mechanisms for uptake of extracellular lipoprotein cholesterol; one is receptor mediated endocytosis and the second is a non-endocytotic pathway. The latter was revealed by the ability of lipoproteins such as HDL which do not possess apo B or apo E to enhance steroid hormo...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435808409036509
更新日期:1984-01-01 00:00:00
abstract::Recent studies from our laboratory have shown that human chorionic gonadotropin (hCG), human luteinizing hormone (hLH), and CG-like proteins from Xanthomonas maltophilia (xCG) and Candida albicans (CaCGLP) induce Candida albicans transition from blastospores to hyphal forms. Xanthomonas maltophilia CG-like protein (xC...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809409035852
更新日期:1994-02-01 00:00:00
abstract::The effect of crowding on the pituitary-gonadal axis was studied in adult male rats. Crowding reduced body weight gain, did not alter relative adrenal weight and increased relative testis weight. Neither basal levels of corticosterone nor its response to acute stress were altered by crowding. Likewise, LH secretion wa...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035431
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:This retrospective and observational study aimed to establish whether any relationship exists between the values of serum thyroid-stimulating hormone (TSH) and those of the mean platelet volume (MPV) in a large cohort of older euthyroid Italian subjects. MATERIALS AND METHOD:The study population was represente...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1037392
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.808207
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Antithyroid drugs such as propylthiouracil (PTU) and methimazole (MMI) are common medications in Chinese patients with hyperthyroidism and PTU-induced antineutrophil cytoplasmic antibody (ANCA) positive vasculitis has been reported. The current cross-sectional study aimed to investigate the prevalence and the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120037729
更新日期:2004-05-01 00:00:00
abstract::The effect of clomiphene on nuclear estrogen receptors of the Fallopian tube during ovum transport in the rabbit has been studied. Nuclear binding capacity was observed in ampulla (A), ampullary-isthmic junction (AIJ), isthmus (I), uterine-isthmic junction (UIJ) and uterus (U). Receptor concentration decreased in all ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808909042745
更新日期:1989-01-01 00:00:00
abstract::We studied whether the activation of rat brown adipose tissue (BAT) by cold exposure or by the administration of beta-3-noradrenergic agonist CGP-12177 could be prevented by the inhibition of thyroxine (T4) to triiodothyronine (T3) conversion. Hypothyroid rats were treated with replacement doses of T4, T4 plus iopanoi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009066164
更新日期:2000-05-01 00:00:00
abstract::In order to clarify the roles of cholinergic and serotonergic neurotransmission in the mediation of exercise-induced growth hormone (GH) release, normal young volunteers of both sexes were studied. Exercise was for 20 minutes at 800 kpm for the men and 500 kpm for the women. Pretreatment with 0.4 mg atropine 1 hour pr...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035433
更新日期:1986-01-01 00:00:00
abstract::A method is described for the preparation of high yields of viable, dissociated cells from porcine theca interna and corpus luteum and from human and bovine endometrium. The tissues were dissociated by incubation at 37 degrees C in a mixture of 0.5% collagenase, 0.1% hyaluronidase and 0.1% pronase in balanced salt sol...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808409035415
更新日期:1984-01-01 00:00:00
abstract:PURPOSE:Adiponectin and chemerin have been reported their associations with insulin resistance and chronic inflammation. However, the relationship between adiponectin and chemerin themselves has not been fully elucidated. Therefore, we investigated the effects of changes in adiponectin and chemerin levels after a weigh...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2017.1300808
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:It has been suggested that the changes in hormone levels that occur with menopause and aging are related to loss of muscle mass. However, the role of gonadotropins in this process is unknown. OBJECTIVES:To analyze the relationship between follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and l...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2012.733987
更新日期:2013-08-01 00:00:00
abstract::Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120018678
更新日期:2003-02-01 00:00:00
abstract::X-linked adrenoleukodystrophy (X-ALD) is the most common peroxisomal disorder characterized by abnormal accumulation of saturated very long chain fatty acids in tissues and body fluids with predominance in brain white matter and adrenal cortex. The clinical phenotype is highly variable ranging from the severe childhoo...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016999
更新日期:2002-11-01 00:00:00