Abstract:
:The steady state level of most cellular phosphoproteins is dependent on the relative catalytic activities of intracellular protein kinases and phosphatases. In adrenal cortex, ACTH acts through PKA activation and Ser/Tre phosphorylation. Phosphatases involved in this pathway are not completely described, particularly the role of phosphotyrosine protein phosphatase (PTP) activity on ACTH action. We investigated potential changes in PTPs activity in adrenal gland upon in vivo and in vitro PKA activation. In vivo ACTH stimulates cytosolic PTP activity (2-fold). Similar effect is detected by in vitro stimulation. In accordance with the effects of ACTH on PTP activity, cell permeable PTP inhibitors block ACTH stimulation on adrenal zona fasciculata (ZF) cells: ACTH (1 nM) = 108.2 +/- 3.5 ng corticosterone/10(5) cells vs. ACTH + phenylarsine oxide (2 nM) = 60 +/- 4 (P < 0.001) and ACTH + pervanadate (10 mM) = 68 +/- 2 (P < 0.01). These results are reproduced when cells are stimulated with cAMP. The inhibition is not observed when steroidogenesis is supported by 22(R)OH cholesterol. We describe, for the first time, a hormonal regulation of PTP activity. According to the effect of PTP inhibitors on steroid production activated by ACTH we propose that PTP activation is a crucial event in hormone action in the steroidogenic pathway. We also propose that PTP activity is located after PKA activation and prior to cholesterol transport to the inner mitochondrial membrane.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Cornejo Maciel F,Paz C,Neuman MI,Podestá EJdoi
10.3109/07435809809032618subject
Has Abstractpub_date
1998-08-01 00:00:00pages
381-6issue
3-4eissn
0743-5800issn
1532-4206journal_volume
24pub_type
杂志文章abstract::Adrenocortical steroidogenesis is regulated in addition to a central regulation via the hypothalamus-pituitary-adrenal axis by intra-adrenal mechanisms involving the adrenal medulla. We could previously show that adrenocortical steroidogenesis is stimulated by co-culturing bovine adrenocortical cells with medullary ch...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048606
更新日期:2000-11-01 00:00:00
abstract::Atrial natriuretic peptide (ANP) is released from the atria and acts to regulate blood volume and pressure. The release of ANP appears to be stimulated by atrial distension, initiated by stretch on the cardiocytes. The purpose of the present study was to develop an assay that would allow for the detection of ANP relea...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809009033011
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:This retrospective and observational study aimed to establish whether any relationship exists between the values of serum thyroid-stimulating hormone (TSH) and those of the mean platelet volume (MPV) in a large cohort of older euthyroid Italian subjects. MATERIALS AND METHOD:The study population was represente...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1037392
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Antithyroid drugs such as propylthiouracil (PTU) and methimazole (MMI) are common medications in Chinese patients with hyperthyroidism and PTU-induced antineutrophil cytoplasmic antibody (ANCA) positive vasculitis has been reported. The current cross-sectional study aimed to investigate the prevalence and the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120037729
更新日期:2004-05-01 00:00:00
abstract::Y1 cells derived from mouse tumor zona fasciculata cells (ZF) were used to assess the importance of extracellular (EC) calcium availability via voltage-dependent calcium channels (VDCC) on steroidogenesis. The steroidogenic response to ACTH was investigated in the presence of blockers known to affect both calcium and ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043745
更新日期:1996-11-01 00:00:00
abstract::The mechanisms by which SF-1 (Steroidogenic Factor-1) and Dax-1 (Dosage-sensitive sex reversal-Adrenal hypoplasia congenita critical region on the X chromosome) dictate adrenal-specific transcriptional programs are the focus of this laboratory. SF-1-mediated transcription is upregulated by phosphorylation of serine 20...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048628
更新日期:2000-11-01 00:00:00
abstract::Excessive aldosterone secretion in some hypertensive patients may result from abnormal aldosterone synthase (AS) gene regulation in response to changes in dietary sodium intake. We have utilized NCI-H295 cells, which exhibit stable angiotensin-induced aldosterone secretion, for transient transfections with murine AS/h...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030462
更新日期:1995-02-01 00:00:00
abstract::Steroid 21-hydroxylase deficiency is the most frequent cause of congenital adrenal hyperplasia, an inherited inability to synthesize cortisol. Mutations causing this disorder have been characterized by hybridization analysis of patient DNA samples using cDNA and oligonucleotide probes, and by cloning and sequencing of...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808909039099
更新日期:1989-01-01 00:00:00
abstract::A study was conducted to provide information on the characteristics of skeletal muscle glucocorticoid receptors and to determine the response of serum cortisol and thyroxine secretion to colostrum feeding in the neonatal calf. Twenty-four calves (12 males and 12 females) were obtained immediately postpartum and random...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808809036341
更新日期:1988-01-01 00:00:00
abstract::The effects of forskolin on the regulation of steroidogenesis and growth were examined in the Y1 adrenocortical tumor cell line, and the roles of cAMP and cAMP-dependent protein kinase in these actions of forskolin were evaluated. Forskolin, like corticotropin, stimulated steroidogenesis 3-fold and inhibited growth by...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808509032978
更新日期:1985-01-01 00:00:00
abstract::The MA-10 mouse Leydig tumor cell line produces large amounts of steroids only in response to hormonal stimulation while the R2C rat Leydig tumor cell line is constitutively steroidogenic in nature. In an effort to uncover the potential reasons for constitutive steroidogenesis in R2C cells, we have recently shown that...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016813
更新日期:2002-11-01 00:00:00
abstract::The enzyme 3beta-hydroxysteroid dehydrogenase plays a crucial role in the steroidogenic process in the adrenal gland. In the present study we tried to characterize its localization and developmental changes in the rat adrenal cortex during the postnatal period, using immunohistochemical methods. The development of the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066126
更新日期:1999-02-01 00:00:00
abstract::Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120018678
更新日期:2003-02-01 00:00:00
abstract::11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyzes the interconversion of cortisol (F) to inactive cortisone (E) in man (corticosterone (B) to 11-dehydrocorticosterone (A) in rodents) and plays a crucial role in regulating corticosteroid hormone action. Two isoforms of this enzyme have been characterized; a ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043775
更新日期:1996-11-01 00:00:00
abstract::Neutrophils have an important role in the host defense. The elevated serum glucose levels of diabetics affect traditional host defenses such as neutrophil counts and functions. The causes of these impairments are not clear. We aimed to investigate changes of peripheral neutrophil counts and functions and their relatio...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809909066155
更新日期:1999-08-01 00:00:00
abstract::T3 receptors are ligand-dependent transcriptional activators that can stimulate gene expression resulting in growth and development. In this study T3 binding sites on isolated liver nuclei are demonstrated in the teleost Anabas testudineus. The effect of hypothyroidism and feeding of polyunsaturated fatty acids (PUFA)...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100106010
更新日期:2001-08-01 00:00:00
abstract::Y1 adrenocortical tumor cells possess amplified and overexpressed c-Ki-ras proto-oncogene, displaying chronic high levels of the c-Ki-Ras-GTP protein. Despite this oncogenic lesion, we previously reported that Y1 cells retain tight regulatory mechanisms of cell cycle control typified by the mitogenic response triggere...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200043600
更新日期:2004-11-01 00:00:00
abstract::Two distinct isoforms of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) with respect to enzymatic activity were identified in the ovine liver and kidney. 11 beta-HSD1 (the hepatic isoform) was reversible and NADP(H)-dependent. By contrast, 11 beta-HSD2 (the renal isoform) was unidirectional and NAD-dependent. Ovin...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.3109/07435809509030453
更新日期:1995-02-01 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) levels were found to be associated with obesity, indicating that adipocytes might influence PAI-1 plasma levels. In addition, the 4G/5G promoter polymorphism of the PAI-1 gene possibly modulates PAI-1 gene transcription and, as a consequence, PAI-1 plasma levels. Metabolic par...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200035728
更新日期:2004-08-01 00:00:00
abstract::We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107168
更新日期:2001-02-01 00:00:00
abstract::A 30-residue peptide corresponding to the amino acid sequence of steroidogenesis activator peptide (SAP) from rat Leydig tumor cells has been synthesized by the solid-phase method using Boc protection. SAP is the putative cycloheximide-sensitive, cAMP-regulated mediator of ACTH-stimulated conversion of cholesterol to ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809109027204
更新日期:1991-01-01 00:00:00
abstract::There is controversy concerning the effects of progestins on bone. Norethindrone acetate (NETA) is synthetic progesterone that also has estrogenic and androgenic effects. We tested its effects on hormone levels, lipids and biochemical markers of bone turnover in postmenopausal women who were on estrogen replacement th...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107870
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND:Small dense low-density lipoprotein (sd-LDL) has been demonstrated to be associated with cardiovascular diseases (CVD). The proposed atherogenic properties of hypoadiponectinemia might be mediated through increased sd-LDL. In this study, the associations of sd-LDL with cardiovascular and other complications ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2014.913614
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.808207
更新日期:2014-01-01 00:00:00
abstract:UNLABELLED:It remains difficult to pre-operatively differentiate follicular carcinoma and adenoma even under intraoperative investigations including histopathologic diagnosis of frozen sections. METHODS:We retrospectively compared dual phase thallium (Tl-201) thyroid scan and fine-needle aspiration (FNA) cytology to d...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120025036
更新日期:2003-08-01 00:00:00
abstract::We have recently reported on the activities of glucose-6-phosphate dehydrogenase (G6PD), hexokinase (HK) and carbonic anhydrase I (CA-I) and II (CA-II) isoenzymes obtained from erythrocytes of healthy subjects and untreated patients with hyperthyroid diseases. Also, erythrocyte zinc concentrations were measured. Red b...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120004538
更新日期:2002-02-01 00:00:00
abstract::We studied whether the activation of rat brown adipose tissue (BAT) by cold exposure or by the administration of beta-3-noradrenergic agonist CGP-12177 could be prevented by the inhibition of thyroxine (T4) to triiodothyronine (T3) conversion. Hypothyroid rats were treated with replacement doses of T4, T4 plus iopanoi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009066164
更新日期:2000-05-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00
abstract::Pertussis toxin (PT) and cholera toxin (CT) have been shown to modulate lactogenic hormone-stimulated Nb2 cell mitogenesis, a lactogen-dependent cell line. As both toxins have been shown to alter guanylate cyclase activity in other cell systems, cyclic guanosine monophosphate (cGMP) analogs, 8-bromo or dibutyryl cGMP,...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809209035926
更新日期:1992-01-01 00:00:00
abstract::We established cell lines from adrenal tumors of transgenic mice harboring the large T-antigen of simian virus 40 under the control of the adrenocortical specific promoter of the scavenger aldose reductase-like akr1b7 gene. Mass spectrometry analyses of serum-supplemented or serum-free culture media showed that ATC1 l...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044168
更新日期:2004-11-01 00:00:00