Abstract：
:The determination of DNA ploidy and the study of the cell cycle in adrenocortical tumoral cells could help in the distinction between benign and malignant lesions and also in the prediction of the biological behaviour of these tumors. We analysed 32 cases of adrenal tissue (8 normal adrenals--N, 12 benign adenomas--A and 12 carcinomas--C). DNA was quantified by image analysis of Feulgen stained sections (Ahrens System) employing ACAS3 software. The DNA content was considered to be diploid in 70% of the N and in 67% of the A groups and in none of C. In this latter group nearly 90% were triploid or tetraploid while this did not occur in any of the A cases. The percentage of cases with a 5c-exceeding rate (5cER) above 5% was nil in the N and A groups and 100% in C. In what concerns the distribution in the cell cycle we found a very distinctive pattern between the groups as the percentage of cases in which the S-phase fraction exceeded 33% was nil in the normals, 8% in A and 83% in the C cases. In conclusion, there was a good correlation between the analysed parameters and the clinically defined groups of adrenal cortex tumors.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Pignatelli D,Leitão D,Maia M,Schmidt Fdoi
10.3109/07435809809032700subject
Has Abstractpub_date
1998-08-01 00:00:00pages
869-74issue
3-4eissn
0743-5800issn
1532-4206journal_volume
24pub_type
杂志文章abstract：PURPOSE:This retrospective and observational study aimed to establish whether any relationship exists between the values of serum thyroid-stimulating hormone (TSH) and those of the mean platelet volume (MPV) in a large cohort of older euthyroid Italian subjects. MATERIALS AND METHOD:The study population was represente...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800.2015.1037392
更新日期：2015-01-01 00:00:00
abstract：:A 30-residue peptide corresponding to the amino acid sequence of steroidogenesis activator peptide (SAP) from rat Leydig tumor cells has been synthesized by the solid-phase method using Boc protection. SAP is the putative cycloheximide-sensitive, cAMP-regulated mediator of ACTH-stimulated conversion of cholesterol to ...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435809109027204
更新日期：1991-01-01 00:00:00
abstract：:The steady state level of most cellular phosphoproteins is dependent on the relative catalytic activities of intracellular protein kinases and phosphatases. In adrenal cortex, ACTH acts through PKA activation and Ser/Tre phosphorylation. Phosphatases involved in this pathway are not completely described, particularly ...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435809809032618
更新日期：1998-08-01 00:00:00
abstract：:Basal serum concentrations of prolactin (PRL) and the elevation of serum PRL concentrations induced by the serotonergic agents, quipazine and 5-hydroxytryptophan (5-HTP) were suppressed in rats treated acutely with dexamethasone. Acute dexamethasone treatment did not alter the stimulation of PRL release produced by th...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435808709023671
更新日期：1987-01-01 00:00:00
abstract：:Standard replacement for adrenal insufficiency (AI) consists of glucocorticoids and mineralocorticoids while DHEA deficiency is routinely ignored. Thus, AI represents the ideal pathophysiological model of isolated DHEA deficiency. We investigated the effects of DHEA replacement in 24 women with primary and secondary A...
journal_title：Endocrine research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.3109/07435800009048561
更新日期：2000-11-01 00:00:00
abstract：:StAR increases the flow of cholesterol from the outer to inner mitochondrial membrane (OMM to IMM), but its mechanism of action remains unclear. MLN64 is a 445 amino acid protein of unknown function that has four N-terminal transmembrane domains and whose C-terminal domain from 218-445 is 37% identical to StAR. N-62 S...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800009048583
更新日期：2000-11-01 00:00:00
abstract：:Combined partial deficiency of 17alpha-hydroxylase and 21-hydroxylase activities was first described in 1985; however the genes for P450c17 and P450c21 in these patients lack mutations. In 1986 we postulated that this disorder might be due to mutations in P450 oxidoreductase (POR), the flavoprotein that donates electr...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-200044134
更新日期：2004-11-01 00:00:00
abstract：:In order to investigate the regulation of connexin distribution in rapidly growing cells, we evaluated the effects of dibutyryl cyclic adenosine monophosphate (DbcAMP) treatment on the cellular location of endogenous connexin 43 (Cx43) in an adrenal tumor cell population. Polyclonal antibodies to Cx43 and a Cx43 green...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-120016983
更新日期：2002-11-01 00:00:00
abstract：:The recent cloning of a family of melanocortin receptors (MC-R) has identified five distinct G protein- and adenylate cyclase-coupled receptors. The MC2-receptor (MC2-R) preferentially binds ACTH. It is expressed in the adrenal cortex and is hence considered to be the ACTH receptor. The MC5-receptor (MC5-R) binds ACTH...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435809809032628
更新日期：1998-08-01 00:00:00
abstract：:There are striking similarities between Cushing's syndrome and the 'metabolic syndrome X' since both are characterised by hypertension, insulin resistance, glucose intolerance, hyperlipidaemia, and central obesity. The possibility that cortisol contributes to the associations between multiple risk factors for cardiova...
journal_title：Endocrine research
pub_type： 杂志文章,评审
doi：10.1080/07435809609043765
更新日期：1996-11-01 00:00:00
abstract：:Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adrenal tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequences contained a termination codon in exon one. The cloned cDNAs were expressed in COS 1 cell...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800009048632
更新日期：2000-11-01 00:00:00
abstract：:Dehydroepiandrosterone sulphate (DHEAS) is a steroid product of the adrenal gland, which circulates in high concentrations, but whose functions are largely unknown. There is evidence for antiproliferative effects of DHEAS in neoplastic tissue. The present study was designed to investigate the effects of DHEAS on vascu...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-200043956
更新日期：2004-11-01 00:00:00
abstract：:A method is described for the preparation of high yields of viable, dissociated cells from porcine theca interna and corpus luteum and from human and bovine endometrium. The tissues were dissociated by incubation at 37 degrees C in a mixture of 0.5% collagenase, 0.1% hyaluronidase and 0.1% pronase in balanced salt sol...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435808409035415
更新日期：1984-01-01 00:00:00
abstract：:Biochemical mechanisms by which analogs of thyrotropin-releasing hormone (TRH) produce their potent neuropharmacological actions on the brain remain ill-defined. We tested effects of YM14673, a novel analog of TRH, on TRH receptors in rat brains in vitro. No significant binding of [3H]YM14673 to brain plasma membranes...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435809509030493
更新日期：1995-11-01 00:00:00
abstract：:Excessive aldosterone secretion in some hypertensive patients may result from abnormal aldosterone synthase (AS) gene regulation in response to changes in dietary sodium intake. We have utilized NCI-H295 cells, which exhibit stable angiotensin-induced aldosterone secretion, for transient transfections with murine AS/h...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435809509030462
更新日期：1995-02-01 00:00:00
abstract：:Several lines of evidence have suggested that the nuclear receptor Steroidogenic Factor-1 (SF-1 or Ad4BP) may be directly involved in the cAMP-dependent regulation of steroid hydroxylase genes in adrenocortical cells. In the bovine CYP17 gene, which encodes the cytochrome P450 17alpha-hydroxylase, an SF-1 site is pres...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435809809032638
更新日期：1998-08-01 00:00:00
abstract：PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800.2016.1141936
更新日期：2016-11-01 00:00:00
abstract：:Adrenal computed tomography with determination of Hounsfield units has proved to be sensitive and specific in the differential diagnosis of benign vs. malign adrenal lesions. On the other hand, computed tomography may fail in patients with small adrenal masses of less than 1.0 cm. However, especially in patients with ...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-120017075
更新日期：2002-11-01 00:00:00
abstract：:Sutherlandia frutescens (Cancer bush), a Southern African indigenous plant, is traditionally used to treat stress related maladies linked to the endocrine system. Extracts of the shrub were used to investigate the claimed stress-relieving properties of the shrub. Dysregulation of the stress response is associated with...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-200044020
更新日期：2004-11-01 00:00:00
abstract：:We quantitated serial serum beta-hydroxybutyrate (beta-OHB) levels using the Ketosite method in 9 children with IDDM who were treated for diabetic ketoacidosis (DKA) and compared them to urinary ketone measurements by dipstick. Persistent elevations of serum beta-OHB were seen in six patients when the urine became cle...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-100107168
更新日期：2001-02-01 00:00:00
abstract：:The present work was to study if the alpha-melanocyte-stimulating hormone (alpha-MSH) was involved in activation of the pituitary-adrenal axis (PAA) in rats. The hormone increased plasma corticosterone (CORT) level, and induced an anxiogenic response as indicated by results from the elevated plus-maze test. Intracereb...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800009066172
更新日期：2000-08-01 00:00:00
abstract：:The enzyme 3beta-hydroxysteroid dehydrogenase plays a crucial role in the steroidogenic process in the adrenal gland. In the present study we tried to characterize its localization and developmental changes in the rat adrenal cortex during the postnatal period, using immunohistochemical methods. The development of the...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435809909066126
更新日期：1999-02-01 00:00:00
abstract：:The cdc2 kinase family is known to be one of the important factors for cell proliferation in both yeast and mammalian cells. By using polyclonal antibodies against PSTAIRE region of cdc2HS, we studied the amount of cdc2-related kinases in the rat thyroid cell line, FRTL-5, during the cell cycle. The immunoreactive pro...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435809409030404
更新日期：1994-05-01 00:00:00
abstract：INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435800.2013.808207
更新日期：2014-01-01 00:00:00
abstract：:11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyzes the interconversion of cortisol (F) to inactive cortisone (E) in man (corticosterone (B) to 11-dehydrocorticosterone (A) in rodents) and plays a crucial role in regulating corticosteroid hormone action. Two isoforms of this enzyme have been characterized; a ...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435809609043775
更新日期：1996-11-01 00:00:00
abstract：:We report a novel mutant of the luteinizing hormone receptor (LHR) in a case of familial Leydig cell hypoplasia and pseudohermaphrotidism. The proband was homozygous for two missense mutations, T1121C and C1175T, causing substitutions I374T and T3921. The molecular effects of the mutations were investigated by heterol...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435800500430890
更新日期：2005-01-01 00:00:00
abstract：:Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-120018678
更新日期：2003-02-01 00:00:00
abstract：:The B vitamin nicotinamide (NIC), commonly known as niacin, is currently in trial as a potential means of preventing Type 1 diabetes in first-degree relatives of affected individuals. Sodium butyrate (BUT) a common dietary micronutrient has also been reported to have beneficial effects on the differentiation and funct...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1081/erc-120028485
更新日期：2004-02-01 00:00:00
abstract：:The effect of clomiphene on nuclear estrogen receptors of the Fallopian tube during ovum transport in the rabbit has been studied. Nuclear binding capacity was observed in ampulla (A), ampullary-isthmic junction (AIJ), isthmus (I), uterine-isthmic junction (UIJ) and uterus (U). Receptor concentration decreased in all ...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.3109/07435808909042745
更新日期：1989-01-01 00:00:00
abstract：:Neutrophils have an important role in the host defense. The elevated serum glucose levels of diabetics affect traditional host defenses such as neutrophil counts and functions. The causes of these impairments are not clear. We aimed to investigate changes of peripheral neutrophil counts and functions and their relatio...
journal_title：Endocrine research
pub_type： 杂志文章
doi：10.1080/07435809909066155
更新日期：1999-08-01 00:00:00
