Effects of resin or charcoal treatment on fetal bovine serum and bovine calf serum.

Abstract:

INTRODUCTION:Charcoal- or resin-stripping of fetal bovine serum (FBS) or bovine calf serum (BCS) intended for supplementation of cell culture media is widely practiced to remove a variety of endogenous compounds, including steroid, peptide, and thyroid hormones. The possibility that stripping removes other biologically relevant factors from serum may not be appreciated. METHODS:In this report, standardized clinical laboratory testing methods were used to assess the effects of resin- and charcoal-stripping on content in FBS and BCS of more than 25 analytes in the sera. RESULTS AND CONCLUSION:In addition to hormones, the serum constituents affected by stripping are certain vitamins, electrolytes, enzyme activities, and metabolites.

journal_name

Endocr Res

journal_title

Endocrine research

authors

Cao Z,West C,Norton-Wenzel CS,Rej R,Davis FB,Davis PJ,Rej R

doi

10.3109/07435800903204082

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

101-8

issue

4

eissn

0743-5800

issn

1532-4206

pii

10.3109/07435800903204082

journal_volume

34

pub_type

杂志文章
  • Diabetic ketoacidosis promotes a prothrombotic state.

    abstract::Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-120018678

    authors: Carl GF,Hoffman WH,Passmore GG,Truemper EJ,Lightsey AL,Cornwell PE,Jonah MH

    更新日期:2003-02-01 00:00:00

  • The effects of growth hormone on corpus luteum of superovulated rats.

    abstract::In general, growth hormone acts as a factor promoting cell proliferation in the positive direction and suppresses apoptosis. No report has described growth hormone (GH)-induced structural luteolysis. The present studies showed that GH induced structural luteolysis in rats after the induction of functional luteolysis b...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435809909066140

    authors: Kiya T,Endo T,Henmi H,Goto T,Kitajima Y,Manase K,Takahashi S,Kudo R

    更新日期:1999-05-01 00:00:00

  • Influence of gender on intrinsic contractile properties of isolated ventricular myocytes from calmodulin-induced diabetic transgenic mice.

    abstract::Diabetes mellitus impairs ventricular function, which itself may be disparately influenced by gender. This study compared the impact of gender on cardiac contractile response in ventricular myocytes from wild-type FVB and calmodulin-induced diabetic transgenic (OVE26) mice at young (2 month) and older (11 month) age. ...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-120022318

    authors: Zhang X,Ye G,Duan J,Chen AF,Ren J

    更新日期:2003-05-01 00:00:00

  • Functional characterization of the adrenoleukodystrophy protein (ALDP) and disease pathogenesis.

    abstract::X-linked adrenoleukodystrophy (X-ALD) is the most common peroxisomal disorder characterized by abnormal accumulation of saturated very long chain fatty acids in tissues and body fluids with predominance in brain white matter and adrenal cortex. The clinical phenotype is highly variable ranging from the severe childhoo...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-120016999

    authors: Gärtner J,Dehmel T,Klusmann A,Roerig P

    更新日期:2002-11-01 00:00:00

  • Expression of type 1 angiotensin II receptors in human aldosteronomas.

    abstract::Type 1 angiotensin II (AII) receptors (AT1 receptors), besides stimulation of aldosterone secretion, seem to transduce the growth factor-like activity of AII on glomerulosa cells. Although a local renin-angiotensin system and AII synthesis have been found in human adrenals and aldosteronomas, it is unclear whether ald...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435809509030434

    authors: Sarzani R,Opocher G,Dessì-Fulgheri P,Paci V,Cola G,Rocco S,Vianello B,Mantero F,Rappelli A

    更新日期:1995-02-01 00:00:00

  • Pharmacological profile of serotonergic receptors in the adrenal gland.

    abstract::The secretory activity of the adrenal gland is mainly regulated by peptidergic hormones (ACTH, angiotensin II) and ions. However, there is now increasing evidence that local factors, including neuropeptides and neurotransmitters, can also participate in the control of adrenocortical cells. In particular, serotonin (5-...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435809809032670

    authors: Delarue C,Contesse V,Lefebvre H,Lenglet S,Grumolato L,Kuhn JM,Vaudry H

    更新日期:1998-08-01 00:00:00

  • Release of atrial natriuretic peptide from individual rat cardiocytes.

    abstract::Atrial natriuretic peptide (ANP) is released from the atria and acts to regulate blood volume and pressure. The release of ANP appears to be stimulated by atrial distension, initiated by stretch on the cardiocytes. The purpose of the present study was to develop an assay that would allow for the detection of ANP relea...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435809009033011

    authors: Miller HA 3rd,Southerland CM

    更新日期:1990-01-01 00:00:00

  • Pref-1, SF-1 and adrenocortical zonation.

    abstract::The stimulation of the zona glomerulosa phenotype by a low sodium diet is characterised by increased expression of aldosterone synthase accompanied by increases in (pro)renin, bFGF, c-fos and c-jun gene transcription. In contrast ACTH diminishes the specific glomerulosa phenotype. The EGF related transmembrane protein...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435809809032720

    authors: Raza FS,Puddefoot JR,Vinson GP

    更新日期:1998-08-01 00:00:00

  • Abundant existence of 40kD-cdc2-related protein in rat thyroid cells.

    abstract::The cdc2 kinase family is known to be one of the important factors for cell proliferation in both yeast and mammalian cells. By using polyclonal antibodies against PSTAIRE region of cdc2HS, we studied the amount of cdc2-related kinases in the rat thyroid cell line, FRTL-5, during the cell cycle. The immunoreactive pro...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435809409030404

    authors: Takano T,Takada K,Tada H,Nishiyama S,Amino N

    更新日期:1994-05-01 00:00:00

  • Calcium regulates human CYP11B2 transcription.

    abstract::The CYP11B2 gene encodes aldosterone synthase, a cytochrome P450 (P450aldo) expressed in high levels in the adrenal zona glomerulosa. While the primary physiologic regulators of aldosterone production are circulating angiotensin II (Ang II) and potassium (K+) the action of these agents on CYP11B2 gene transcription ha...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435809609043735

    authors: Clyne CD,White PC,Rainey WE

    更新日期:1996-11-01 00:00:00

  • Partial rescue of the phenotype in 1α-hydroxylase gene knockout mice by vitamin D3 injection.

    abstract:OBJECTIVE:To investigate whether 25-hydroxyvitamin D [25(OH)D] can mediate effects without being converted to 1α,25-dihydroxyvitamin D [1,25(OH)2D]. METHODS:Vitamin D3 (VD3) was injected intramuscularly to 25-hydroxyvitamin D-1α-hydroxylase [1α(OH)ase] gene knockout (KO) male mice with a dose of 10,000 IU per week for...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800.2010.542415

    authors: Zhang ZL,Ding XF,Tong J,Li BY

    更新日期:2011-01-01 00:00:00

  • DHEA replacement in women with adrenal insufficiency--pharmacokinetics, bioconversion and clinical effects on well-being, sexuality and cognition.

    abstract::Standard replacement for adrenal insufficiency (AI) consists of glucocorticoids and mineralocorticoids while DHEA deficiency is routinely ignored. Thus, AI represents the ideal pathophysiological model of isolated DHEA deficiency. We investigated the effects of DHEA replacement in 24 women with primary and secondary A...

    journal_title:Endocrine research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/07435800009048561

    authors: Arlt W,Callies F,Allolio B

    更新日期:2000-11-01 00:00:00

  • Effect of vitamin D supplementation on muscle energy phospho-metabolites: a ³¹P magnetic resonance spectroscopy-based pilot study.

    abstract::There are several published reports on the prevalence of low vitamin D levels in otherwise healthy Indian population. Vitamin D deficiency has shown variable effect on muscle performance and strength but there is paucity of data on the effect of vitamin D deficiency on muscle energy metabolism. The present study was p...

    journal_title:Endocrine research

    pub_type: 临床试验,杂志文章

    doi:10.3109/07435800.2013.865210

    authors: Rana P,Marwaha RK,Kumar P,Narang A,Devi MM,Tripathi RP,Khushu S

    更新日期:2014-01-01 00:00:00

  • Sexually dimorphic expression of co-repressor Sin3A in mouse kidneys.

    abstract::Using Western blot analysis we found transcriptional co-repressor Sin3A to be expressed at a higher level in male mouse kidney than in females. HDAC1 (histone deacetylase 1) protein, another co-repressor forming complexes with Sin3A, was not higher in males. No sex differences in Sin3A expression were found after gona...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435800500229243

    authors: Xu J,Arnold AP

    更新日期:2005-01-01 00:00:00

  • ACTH inhibits A Ras-dependent anti-apoptotic and mitogenic pathway in mouse Y1 adrenocortical cells.

    abstract::Mouse Y1 adrenocortical tumor cells harbor amplified and overexpressed c-Ki-ras gene, displaying relatively high constitutive levels of Ras x GTP. Here we report that Y1 cells also constitutively display high levels of phosphorylated AKT/PKB, that are dependent on Ras x GTP and PI3K. ACTH rapidly causes dephosphorylat...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800009048616

    authors: Forti FL,Armelin HA

    更新日期:2000-11-01 00:00:00

  • Coordinated regression of adrenocortical endocrine and endothelial compartments under adrenocorticotropin deprivation.

    abstract::The adrenal cortex is a complex tissue comprising different cell types, including endocrine, stromal, nerve, perivascular, and endothelial cells. The pituitary hormone ACTH controls the coordinated development of the vasculature and the endocrine tissue mass. This suggests that paracrine secretions between steroidogen...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-200043623

    authors: Keramidas M,Feige JJ,Thomas M

    更新日期:2004-11-01 00:00:00

  • Influence of progesterone and its antagonist mifepristone (RU 486) on collagen content in uterine cervix in pregnant rats.

    abstract::Total collagen and its soluble fraction as well as water content were determined in late pregnancy in the uterine cervix in a population of 36 rats treated with progesterone (n = 10), mifepristone (n = 13) and controls (n = 13). In both treated groups there were no signs of delivery on the 21st day of gestation. Water...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435809509030477

    authors: Bieńkiewicz A

    更新日期:1995-08-01 00:00:00

  • Dehydroepiandrosterone sulphate (DHEAS) inhibits growth of human vascular endothelial cells.

    abstract::Dehydroepiandrosterone sulphate (DHEAS) is a steroid product of the adrenal gland, which circulates in high concentrations, but whose functions are largely unknown. There is evidence for antiproliferative effects of DHEAS in neoplastic tissue. The present study was designed to investigate the effects of DHEAS on vascu...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-200043956

    authors: Hinson JP,Khan M

    更新日期:2004-11-01 00:00:00

  • P450aldo in hamster adrenal cortex: immunofluorescent and immuno-gold electron microscopic studies.

    abstract::The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. O...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435809509030443

    authors: LeHoux JG,Martel D,LeHoux J,Ducharme L,Lefebvre A,Brière N

    更新日期:1995-02-01 00:00:00

  • Modulation of T-Bet and GATA-3 expression in experimental autoimmune thyroiditis rats through ginsenoside treatment.

    abstract::Hashimoto's thyroiditis (HT) is one of the most common organ-specific autoimmune diseases. Increasing evidence indicates that HT may be characterized by an imbalance in the helper T cell subsets Th1 and Th2. Traditional Chinese Medicine (TCM) considers HT as a chronic exhaustion disease, leading to deficiency of qi. I...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800.2015.1066800

    authors: Chen J,Feng X,Huang Q

    更新日期:2016-01-01 00:00:00

  • Molecular mechanisms of actions of formulations of the thyroid hormone analogue, tetrac, on the inflammatory response.

    abstract:BACKGROUND:Tetraiodothyroacetic acid (tetrac) and its nanoparticulate formulation (Nanotetrac) act at a cell surface receptor to block angiogenesis and tumor cell proliferation. OBJECTIVE:The complex anti-angiogenic properties of tetrac and Nanotetrac caused us to search in the literature and in certain of our unpubli...

    journal_title:Endocrine research

    pub_type: 杂志文章,评审

    doi:10.3109/07435800.2013.778865

    authors: Davis PJ,Glinsky GV,Lin HY,Incerpi S,Davis FB,Mousa SA,Tang HY,Hercbergs A,Luidens MK

    更新日期:2013-01-01 00:00:00

  • Positive association between the changes in chemerin and adiponectin levels after weight reduction.

    abstract:PURPOSE:Adiponectin and chemerin have been reported their associations with insulin resistance and chronic inflammation. However, the relationship between adiponectin and chemerin themselves has not been fully elucidated. Therefore, we investigated the effects of changes in adiponectin and chemerin levels after a weigh...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435800.2017.1300808

    authors: Shin HY,Park S,Lee JW

    更新日期:2017-11-01 00:00:00

  • Baboon cytochrome P450c11 is encoded by more than one gene.

    abstract::Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adrenal tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequences contained a termination codon in exon one. The cloned cDNAs were expressed in COS 1 cell...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800009048632

    authors: Swart AC,Brown N,Kolar N,Swart P

    更新日期:2000-11-01 00:00:00

  • Lymph node fine-needle aspiration washout thyroglobulin in papillary thyroid cancer: Diagnostic value and the effect of thyroglobulin antibodies.

    abstract:PURPOSE:Thyroglobulin (Tg) assessment in the needle washout after fine-needle aspiration biopsy (FNAB) of a suspicious neck lymph node (LN) is known to improve the diagnostic accuracy in patients with papillary thyroid cancer (PTC). However, there is still controversy on the best diagnostic cut-off levels for FNAB-Tg a...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800.2016.1141936

    authors: Konca Degertekin C,Yalcin MM,Cerit T,Ozkan C,Kalan I,Iyidir OT,Altinova AE,Akturk M,Toruner F,Akin M,Cakir N

    更新日期:2016-11-01 00:00:00

  • Characterization and purification of the chorionic gonadotropin-like protein binding site in Candida albicans.

    abstract::Recent studies from our laboratory have shown that human chorionic gonadotropin (hCG), human luteinizing hormone (hLH), and CG-like proteins from Xanthomonas maltophilia (xCG) and Candida albicans (CaCGLP) induce Candida albicans transition from blastospores to hyphal forms. Xanthomonas maltophilia CG-like protein (xC...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435809409035852

    authors: Caticha O,Odell WD

    更新日期:1994-02-01 00:00:00

  • Cytosolic glucocorticoid receptors in skeletal muscle of the newborn calf.

    abstract::A study was conducted to provide information on the characteristics of skeletal muscle glucocorticoid receptors and to determine the response of serum cortisol and thyroxine secretion to colostrum feeding in the neonatal calf. Twenty-four calves (12 males and 12 females) were obtained immediately postpartum and random...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435808809036341

    authors: Waggoner DK,McCarthy FD,Akers RM

    更新日期:1988-01-01 00:00:00

  • A novel double mutation in the luteinizing hormone receptor in a kindred with familial Leydig cell hypoplasia and male pseudohermaphroditism.

    abstract::We report a novel mutant of the luteinizing hormone receptor (LHR) in a case of familial Leydig cell hypoplasia and pseudohermaphrotidism. The proband was homozygous for two missense mutations, T1121C and C1175T, causing substitutions I374T and T3921. The molecular effects of the mutations were investigated by heterol...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1080/07435800500430890

    authors: Pals-Rylaarsdam R,Liu G,Brickman W,Duranteau L,Monroe J,El-Awady MK,Gad YZ,Shenker A

    更新日期:2005-01-01 00:00:00

  • Thyrotropin as an independent factor of renal function and chronic kidney disease in normoglycemic euthyroid adults.

    abstract:OBJECTIVES:The relationship between thyroid status and renal function has been well characterized. However, renal function is affected by various factors, including sex and dysglycemia. Because these studies have yet to be undertaken in a normoglycemic population, this study sought to explore the association between th...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.3109/07435800.2011.640374

    authors: Sun MT,Hsiao FC,Su SC,Pei D,Hung YJ

    更新日期:2012-01-01 00:00:00

  • Effect of micronized progesterone on bone turnover in postmenopausal women on estrogen replacement therapy.

    abstract:PURPOSE:To determine whether daily oral micronized progesterone affects bone turnover, as estimated by serum and urine biochemical markers, in postmenopausal women on long-term estrogen replacement therapy (ERT). METHODS:We recruited 14 women aged 65 or older to participate in a 9-week trial with micronized progestero...

    journal_title:Endocrine research

    pub_type: 临床试验,杂志文章

    doi:10.1081/erc-120022294

    authors: Azizi G,Hansen A,Prestwood KM

    更新日期:2003-05-01 00:00:00

  • Difference of in vivo and in vitro antimineralocorticoid potency of progesterone.

    abstract::Progesterone (P) given intramuscularly increases renal sodium excretion. We tested the in vitro capacity of P to bind to the human mineralocorticoid receptor (MR) with a reticulocyte lysate system and Ps transactivation potency in transfected CV-1 cells. Progesterone binds with higher affinity to the MR than aldostero...

    journal_title:Endocrine research

    pub_type: 杂志文章

    doi:10.1081/erc-120016824

    authors: Quinkler M,Diederich S

    更新日期:2002-11-01 00:00:00