Aldehyde fixative solutions alter the water relaxation and diffusion properties of nervous tissue.

Abstract:

:Chemically-fixed nervous tissues are well-suited for high-resolution, time-intensive MRI acquisitions without motion artifacts, such as those required for brain atlas projects, but the aldehyde fixatives used may significantly alter tissue MRI properties. To test this hypothesis, this study characterized the impact of common aldehyde fixatives on the MRI properties of a rat brain slice model. Rat cortical slices immersion-fixed in 4% formaldehyde demonstrated 21% and 81% reductions in tissue T(1) and T(2), respectively (P < 0.001). The T(2) reduction was reversed by washing slices with phosphate-buffered saline (PBS) for 12 h to remove free formaldehyde solution. Diffusion MRI of cortical slices analyzed with a two-compartment analytical model of water diffusion demonstrated 88% and 30% increases in extracellular apparent diffusion coefficient (ADC(EX)) and apparent restriction size, respectively, when slices were chemically-fixed with 4% formaldehyde (P

journal_name

Magn Reson Med

authors

Shepherd TM,Thelwall PE,Stanisz GJ,Blackband SJ

doi

10.1002/mrm.21977

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

26-34

issue

1

eissn

0740-3194

issn

1522-2594

journal_volume

62

pub_type

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