Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.

Abstract:

OBJECTIVE:To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms. DESIGN:AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay. In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms. RESULTS:Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized. They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p <  0.05) at concentrations > 15 μg/mL. In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control. We also demonstrated significant (p <  0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs. CONCLUSION:AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Madla-Cruz E,De la Garza-Ramos M,Romo-Sáenz CI,Tamez-Guerra P,Garza-Navarro MA,Urrutia-Baca V,Martínez-Rodríguez MA,Gomez-Flores R

doi

10.1016/j.archoralbio.2020.104943

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

104943

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(20)30321-6

journal_volume

120

pub_type

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