Abstract：
OBJECTIVE:Syndecan-1 plays an important role in cell proliferation in dental papilla during tooth development. This study aimed to clarify the transcription mechanisms that regulate syndecan-1 gene expression in dental papilla. DESIGN:We analysed genomic conservation and putative transcriptional factor binding sites of syndecan-1 gene loci using the bioinformatics tool VISTA. To identify the region responsible for syndecan-1 gene expression in mouse dental papilla cells (MDPCs) in vitro, the 1.5-kb upstream region of the mouse syndecan-1 coding region was inserted upstream of the enhanced green fluorescent protein (EGFP) or luciferase gene, and promoter activity was examined by transient reporter gene expression assay in cultured MDPCs. To examine the binding of the upstream binding factor, we performed chromatin immunoprecipitation (ChIP) assay. RESULTS:VISTA analysis showed that the 1.5-kb upstream region was highly conserved amongst species, and three GC-rich motifs, as well as a TATA-box-like motif, were identified in this region. Reporter gene assay showed that the 1.5-kb upstream region of mouse syndecan-1 induced reporter gene expression in MDPCs. Deletion of the promoter from the 5'-end to 339 bp upstream reduced luciferase activity by nearly half vs. the 1.5-kb sequence. Further deletion up to 68 bp resulted in further loss of luciferase activity. On ChIP assay, we found direct recruitment of Sp3 transcription factor to the GC-rich motif region. CONCLUSION:The 1.5-kb upstream region of the syndecan-1 gene was sufficient to induce its expression in dental papilla, and binding of Sp3 transcription factor may play a pivotal role in this syndecan-1 induction.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Iguchi-Ishiguro H,Ouchi Y,Watanabe S,Numabe Ydoi
10.1016/j.archoralbio.2011.10.017subject
Has Abstractpub_date
2012-05-01 00:00:00pages
531-8issue
5eissn
0003-9969issn
1879-1506pii
S0003-9969(11)00350-5journal_volume
57pub_type
杂志文章abstract：OBJECTIVE:Streptococcus mutans, known to be a major pathogen of dental caries, is also considered to cause infective endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.11.008
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:Changes in the masticatory function of ferrets have been shown to cause alterations in their craniofacial morphology. The aim of the present study was to investigate how the functional changes influence the histochemical characteristics of fibres of the masticatory muscles of the animal. DESIGN:Forty five-we...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.07.011
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVES:This study aimed to evaluate and compare the early biological effects of allogeneic bone marrow-derived mesenchymal stem cells (BMSCs) versus salmon calcitonin (SC) on healing of surgically induced mandibular bone defects in osteoporotic rats. METHODS:Sixty-one female albino rats were included in this study...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104934
更新日期：2020-12-01 00:00:00
abstract：:One hundred and forty-two teeth of children and adults were analysed for lead content by graphite furnace atomic absorption spectrophotometry. The results showed that, for children, tooth age is an important factor that has to be taken into consideration when lead levels are evaluated. In adults tooth type may be igno...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90069-m
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.03.017
更新日期：2013-09-01 00:00:00
abstract：:The impeded/unimpeded eruption rates of the rat incisor have been much studied. The most commonly used optical microscopic method, which has inherent weaknesses, was compared here with an image-analysis method to measure the eruption rate of the mandibular incisor. The study also evaluated the incisal edge as a refere...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00039-0
更新日期：2002-07-01 00:00:00
abstract：:The study examined the glucose clearance (retention) in saliva at different surfaces of these teeth in 23 subjects. The mouth was thoroughly rinsed for 15 s with 20 ml of a 0.5 M glucose solution. The concentrations of glucose absorbed by small pieces of paper, placed on the mesial, distal, labial (buccal), lingual an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90183-m
更新日期：1993-06-01 00:00:00
abstract：:Platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I) interact to stimulate proliferation of fibroblasts in culture. Glucocorticoids variably effect the response of cultured fibroblasts to polypeptide growth factors. This study determined the effects of dexamethasone on growth-factor stimulati...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90009-w
更新日期：1992-02-01 00:00:00
abstract：OBJECTIVE:We have already reported that chitosan inhibited the growth of cariogenic bacteria in vitro. This study was designed to evaluate whether chewing gum, containing chitosan, can effectively suppressed the growth of oral bacteria (total bacteria, total Streptococci, mutans streptococci (MS)) in saliva. METHODS:F...
journal_title：Archives of oral biology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.archoralbio.2006.10.004
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVE:Dental follicle cells (DFCs) are the genuine precursors of alveolar osteoblasts. Previous studies suggested that collagen I supports the osteogenic differentiation of DFCs. This study investigated the effect of collagen I on the osteogenic differentiation of human DFCs. MATERIALS AND METHODS:We modified the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.07.013
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To assess a panel of risk factors associated to oral candidosis (OC) onset and its chronic maintenance by means of fuzzy logic (FL) approach and statistical traditional methodology (STM); to investigate their casual relationship within a multifactorial framework. DESIGN:Case-control study. SETTING:One hospi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.009
更新日期：2008-04-01 00:00:00
abstract：OBJECTIVES:Reconstruct childhood diet using teeth collected in Edmonton in a collaborative effort between the Departments of Dentistry and Anthropology at the University of Alberta. METHODS:Deciduous teeth needing extraction were collected from 33 children for stable isotope ratio analysis of diet. Tooth dentin was mi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.07.014
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the correlation between the immunohistochemical expression of proliferating cell nuclear antigen (PCNA), factor VIII, and CD34 (markers of endothelial cells), and vascular endothelial growth factor (VEGF) and the recurrence of oral squamous cell carcinoma (OSCC) and or...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.04.012
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to clarify the effects of zinc chloride (ZnCl2) exposure on the induction of heme oxygenase-1 (HO-1) expression and its regulatory mechanisms in MDPC-23 mouse odontoblast-like cells. METHODS:MDPC-23 cells were incubated with ZnCl2, and the levels of HO-1 protein, phosphorylated forms...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.09.002
更新日期：2013-04-01 00:00:00
abstract：:The distribution of substance P (SP)-containing synaptic terminals in the hypoglossal nucleus (XII) of adult rats was examined by retrograde peroxidase labelling and immunocytochemistry. From the location of peroxidase injections into the tongue and of labelled neurones in the ventral lamina of XII, motor neurones tha...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00040-0
更新日期：1999-08-01 00:00:00
abstract：OBJECTIVE:We investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks. DESIGN:Dentin samples were prepared (n=120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500mg/mL), low con...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.06.006
更新日期：2016-10-01 00:00:00
abstract：:Garlic (Allium sativum) has long been known to have antibacterial, antifungal and antiviral properties but there are few data on its effects against oral bacterial species particularly putative periodontal pathogens or their enzymes. Filter sterilised, aqueous extract of garlic was tested for ability to inhibit the gr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.12.002
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVE:Studies on the influence of low-power red laser on the repair of dental structures are very scarce. This study investigated the effects of the laser therapy on the ultrastructure of the dentine-pulp interface after conservative class I cavity preparation. DESIGN:Two female volunteers with 8 premolars indicat...
journal_title：Archives of oral biology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.archoralbio.2007.02.003
更新日期：2007-09-01 00:00:00
abstract：:The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that M...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90070-6
更新日期：1985-01-01 00:00:00
abstract：:Intradental, i.e. pulpal, cells may play an important part in sensory transduction in teeth, although the cellular mechanisms and the identity of the specific cell types involved are still unclear. Because the majority of cells in dental pulp are derived from neural crest, it seemed likely that these might have the me...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90137-6
更新日期：1994-07-01 00:00:00
abstract：:Simultaneous records of masseter-EMG activity and position of the mandible were made on 4 Wistar rats eating four food types: standard food pellets (large pellets), 45 mg precision food pellets (small pellets), bread and pudding. Masseter activity was recorded with chronic bipolar silver EMG electrodes placed within t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90060-2
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:It was aimed to investigate the in vivo effects of local injection of sclerostin protein on orthodontic tooth movement. DESIGN:A total of 48 rats underwent orthodontic mesialization of the maxillary first molars on both sides. Local injection was given at the compression side in the alveolar bone on both max...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.12.011
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:It has been suggested that oxidative stress plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD), though this role has yet to be fully elucidated. The purpose of this study was to further evaluate this role as concomitantly expressed in the saliva and broncho-alveolar lavage (BAL/'...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.08.002
更新日期：2007-01-01 00:00:00
abstract：:The classical hydrodynamic theory implicated fluid movement as a transducing mechanism in the production of dental sensitivity. This theory assumes that sensitive dentine must be permeable. Various measurements of dentine permeability are discussed, including: (1) factors that influence diffusive permeation across den...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/0003-9969(94)90191-0
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVES:To assess the association between the promoter region of serotonin transporter (5-HTTLPR) gene polymorphism and recurrent aphthous stomatitis (RAS) occurrence. DESIGNS:We performed the meta-analysis to determine the potential association between 5-HTTLPR polymorphism and RAS. We retrieved revelant studies f...
journal_title：Archives of oral biology
pub_type： 杂志文章,meta分析
doi：10.1016/j.archoralbio.2019.104555
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:This study evaluates the antibacterial activity against mono and multispecies bacterial models and the cytotoxic effects of zinc oxide and copper nanoparticles(ZnO-NPs/Cu-NPs) in cell cultures of human gingival fibroblasts(HGFs). DESIGN:The antibacterial activities of ZnO-NPs and Cu-NPs against 4 bacteria sp...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.105031
更新日期：2021-02-01 00:00:00
abstract：:Radially directed inter-odontoblastic collagen fibres were observed in ultra-thin sections of the radicular pulp/predentine complex of rat molars. Bundles of fibres crossed the distal junctional complexes of the odontoblasts, went through the whole thickness of the predentine and were incorporated into the mineralized...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90082-6
更新日期：1991-01-01 00:00:00
abstract：:The expression and distribution of inducible nitric oxide synthase (iNOS) was examined in 12 samples of human temporomandibular joint (TMJ) with internal derangement (ID) and four control specimens. In the diseased joints, strong or definite iNOS reactivity was expressed in synovial lining and endothelial cells; weake...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00086-8
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE AND DESIGN:Autopsies of the submandibular gland, the vestibular folds and the soft palate from 65-87 old humans were examined to record the immunohistochemical expression of MUC1 and the simple mucin-type antigens Tn and Sialyl-Tn. RESULTS:(1) The serous acini in the submucosal glands from the larynx and the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.07.007
更新日期：2010-11-01 00:00:00
abstract：:Evidence was sought for urea-induced pH gradients in dental plaque microcosm biofilms cultured from the mixed salivary bacteria in a multi plaque 'artificial mouth'. Application of 500 mmol/l urea for short periods (6 min) to 5-8 mm maximum-thickness plaques induced intraplaque pH gradients of up to 0.7 pH units with ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90147-3
更新日期：1994-06-01 00:00:00
