Abstract：
:Culture of the developing dental tissues has contributed to understanding of developmental processes during early odontogenesis. However, to understand fully the mechanisms involved during dentinogenesis and tissue repair there is a need to develop culture models for the dentine-pulp complex from more mature dental tissues. This study describes the development of a system for the organ culture of mature rodent teeth. Slices of incisors from 28-day-old rats were embedded in a semisolid, agar-based medium and cultured on floating Millipore filters at the liquid-gas interface for up to 14 days. Preservation of cell and tissue morphology was observed throughout the entire dentine-pulp complex after each culture period and autoradiographic studies showed that the odontoblasts were actively synthesizing and secreting extracellular matrix during culture. Transmission electron microscopy confirmed that the phenotypic morphology of the odontoblasts had been maintained during culture. These results demonstrate that the dentine-pulp complex from mature rodent tissues can be cultured successfully for substantial periods of time and will provide a useful model for the study of dentinogenesis and tissue repair.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Sloan AJ,Shelton RM,Hann AC,Moxham BJ,Smith AJdoi
10.1016/s0003-9969(98)00029-6subject
Has Abstractpub_date
1998-06-01 00:00:00pages
421-30issue
6eissn
0003-9969issn
1879-1506pii
S0003-9969(98)00029-6journal_volume
43pub_type
杂志文章abstract：:The net surface charge of various oral streptococci were assessed by aqueous two-phase partitioning in a dextran-polyethylene glycol system. Great variability was found among individual strains within all species tested. Type 1 strains of Streptococcus sanguis serotypes which have been found to be more adherent, expos...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(81)90114-x
更新日期：1981-01-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between ATP and IL-6 in mechanical stress-induced REX-1 expression in SHEDs. METHODS:Cells were stimulated with mechanical stress (0-2.5 gcm(-2)), IL-6 (0.1-5 ng/ml), or ATP (10-100 μM) for 2h in serum-free media. IL-6 and REX-1 expression was examined by qualitative and quant...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.09.008
更新日期：2015-01-01 00:00:00
abstract：:An electrophoretic method capable of analysing a small volume (5-10 microliter) of human palatine saliva without prior concentration was developed by combining the high resolution of isoelectric focusing in agarose with a highly sensitive silver protein staining method. Human palatine saliva exhibited 23 protein bands...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90080-8
更新日期：1983-01-01 00:00:00
abstract：:Using this method, without denaturing agents, over 60 protein components in 2 microliter (about 4 micrograms of protein) of unconcentrated parotid saliva could be detected. After two-dimensional separation, the spots of albumin, secretory IgA, IgG, acid phosphatase, amylase and plasma-originating protein were identifi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90048-8
更新日期：1986-01-01 00:00:00
abstract：:The application of detergent solutions to dissociated molar dental papillae of mice permitted precise observation of the extracellular fibrillar material in the odontoblast layer and the predentine. Two types of fibrils were identified: cross-banded fibrils of variable diameter (25-200 nm) and aperiodic microfibrils o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90092-1
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. DESIGN:We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal days 1-100 by ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.09.007
更新日期：2006-05-01 00:00:00
abstract：:Dental fluorosis was evaluated by a classification system, previously shown to be sensitive, and skeletal changes evaluated by bone maturity and structure. Dental fluorosis was more severe in posterior than in anterior teeth in both jaws irrespective of fluoride concentration of the drinking water. There appeared to b...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90004-8
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:The margin of a palatal cleft is a unique anatomical site since the palatal mucosa is continuous with the nasal or nasopharyngeal mucosa. The aim of this study was to compare the expression patterns of cytokeratins and basal membrane components of the mucosa in the area of the cleft. DESIGN:Biopsies from the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.01.004
更新日期：2006-07-01 00:00:00
abstract：OBJECTIVE:Interleukin (IL)-12 is an important cytokine for innate and adaptive immunity. We previously reported that IL-12 inhibits tumour necrosis factor (TNF)-α-mediated osteoclast formation by inducing apoptosis. We also reported that TNF-α plays an important role in mechanical loading-induced osteoclast formation a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.07.006
更新日期：2012-01-01 00:00:00
abstract：:Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00038-q
更新日期：1995-08-01 00:00:00
abstract：:Twenty-five premolars (from Nagoya, Japan < 0.1 parts/10(-6)F in drinking water) were sampled to determine the fluoride content in imbrication lines of Retzius between the grooves and ridges of perikymata on the enamel surface. Eight small windows were formed on each surface in groove and ridge regions using an etched...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(98)00059-4
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to systematically review the literature regarding the prevalence of periodontal diseases and dental caries in patients with leukemia. METHODS:An electronic search for observational studies on oral health outcomes in patients with leukemia was performed on Medline/PUBMED, Embase, Web...
journal_title：Archives of oral biology
pub_type： 杂志文章,meta分析
doi：10.1016/j.archoralbio.2020.104948
更新日期：2020-12-01 00:00:00
abstract：:Dialysed extracts from dental plaque and a number of species of oral streptococci and actinomycetes have been investigated for their effects on cell growth, [3H]-thymidine uptake and 51Cr release from HeLa cell cultures. Eight of the 15 extracts inhibited cell growth and all but one inhibited [3H]-thymidine incorporat...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90178-9
更新日期：1982-01-01 00:00:00
abstract：:The secretions (HPS) contained an arylamidase-like enzyme discovered by chromatography on Sephadex G-100 Superfine columns using N-L-alanyl-2-naphthylamine (2NA) as substrate. The enzyme was fractionated in the void volume, suggesting that its molecular weight was 150,000 or higher. It hydrolysed, with decreasing rate...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90050-0
更新日期：1985-01-01 00:00:00
abstract：:Actinomyces species are predominant early colonizers of the oral cavity and prime mediators of inter-bacterial adhesion and coaggregation. Previous workers have evaluated the adhesion of Actinomyces spp. by quantitative assessment of sessile, as opposed to planktonic cells attached to substrates, but did not quantify ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.04.003
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:Streptococcus mutans, known to be a major pathogen of dental caries, is also considered to cause infective endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.11.008
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:While Aggregatibacter actinomycetemcomitans (Aa) is highly associated with localised aggressive periodontitis (LAP) many Aa-carriers do not develop LAP. This study was designed to determine whether specific salivary factors could distinguish between subjects who have Aa initially and remain healthy (H/AA) as ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.10.009
更新日期：2013-06-01 00:00:00
abstract：:The eruption of the deciduous teeth in Down's individuals is reportedly delayed, but the extent of delay in comparison to normal children has been little studied. The eruption characteristics of the deciduous teeth in a sample of Chilean individuals with Down's syndrome were compared with those of the normal Chilean p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00012-5
更新日期：1997-05-01 00:00:00
abstract：:Fluorescently labelled latex microspheres (0.01, 0.1 and 1.0 micron dia.) were used to establish whether oral epithelial cells could exhibit an endocytic function. Oral mucosa biopsies were incubated in organ culture at 37 degrees C for 20 h with one of the three sizes of fluorescent microspheres in the medium. Tissue...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00027-8
更新日期：1999-06-01 00:00:00
abstract：:Transmitted and polarized light microscopy of unerupted and erupted teeth affected by a pigmented anomaly found in two geographically isolated Polynesian populations, the New Zealand Maori and the French Polynesian Marquesas Islander, showed similar histological characteristics. Mounted in water, the pigmented areas w...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90127-1
更新日期：1989-01-01 00:00:00
abstract：:Candidal adherence to mucosal surfaces is considered as the first step in the pathogenesis of oral candidiasis. We examined the effect of antifungal polyenes, amphotericin B, nystatin and natamycin, at sublethal and minimum inhibitory concentrations (MICs) on the adherence of Candida albicans and Candida glabrata to H...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00174-2
更新日期：2003-12-01 00:00:00
abstract：:A common finding in Swedish children is hypomineralization in first molars of unknown cause. Little is known about the chemical composition of the disturbed enamel. The aim here was to analyse the concentration gradients for F, Cl, Na, Mg, K and Sr in hypomineralized enamel from 17 permanent molars by means of seconda...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00113-8
更新日期：2001-03-01 00:00:00
abstract：:A task-force to resolve 26 pending forensic caseworks was carried out. We tested four different protocols to extract DNA from molar and pre-molar teeth from 26 cadavers with post-mortem intervals from 2 months to 12 years. We compared the amount of DNA and DNA profiles with the time elapsed between death and laborator...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.014
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN:Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.09.005
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:Hyposalivation is a common symptom of diabetes. Although microRNAs (miRNAs) play a role in the pathogenesis of diabetes, the specific effects of miRNAs on diabetic salivary glands are not clear. DESIGN:We used high-throughput technologies to screen differentially expressed (DE) miRNAs and mRNAs in submandibu...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104947
更新日期：2020-12-01 00:00:00
abstract：:Hinokitiol, a natural iron-chelating agent, is known to have diverse biological and pharmacological activities in various cell types. However, the effect of hinokitiol on dental pulp cells has not yet been reported. In this study, hinokitiol increases hypoxia-inducible factor-1α (HIF-1α) protein levels and vascular en...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.10.009
更新日期：2014-02-01 00:00:00
abstract：:The number of Strep. mutans in saliva samples was examined in 101 13-14-year-old children, 53 in a control and 48 in the test group. All in the test group with 2.5 X 10(5) Strep. mutans per ml saliva were treated with 1 per cent chlorhexidine gel, once a day, for 14 days when the number of Strep. mutans was greatly re...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90042-5
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is a birth defect for which several genes susceptibility genes been proposed. Consequently, it has been suggested that many of these genes belong to common inter-related pathways during craniofacial development gene-gene interaction. We evaluated th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.04.009
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to determine if vascular endothelial growth factor (VEGF) can upregulate the gene expression of receptor activator of nuclear factor kappa B (RANK) in osteoclast precursors, as does CSF-1. A secondary aim was to determine if VEGF can promote osteoclastogenesis in vitro comparable to ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.12.006
更新日期：2006-07-01 00:00:00
abstract：:The discharge of albumin, IgG and fibrinogen in dentinal fluid released from freshly exposed dentine was determined by enzyme-linked immunosorbent assay. Data were compared with the relative concentrations of these proteins in the interstitial fluid of the pulp. The sampling procedure involved retrieval of phosphate-b...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90043-4
更新日期：1994-03-01 00:00:00
