Abstract：
OBJECTIVE:This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DESIGN:DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic features were confirmed by flow cytometry for cell markers STRO-1, cluster of differentiation (CD) 146, CD45, and collagen type-I. The proliferation potential of cells was evaluated by 5-bromo-2'-deoxyuridine (brdU) incorporation assay, and finally they were assessed for multi-lineage differentiation potential. Data were analyzed using one-way analysis of variance and independent t-tests. RESULTS:DPSCs isolated by either method showed similar levels of STRO-1, CD45, and collagen type-I and similar incorporation of brdU (P > 0.05). However, DPSCs obtained by collagenase I/dispase treatment had significantly higher numbers of CD146+ cells and osteogenic and chondrogenic capacities compared to those obtained by treatment with collagenase I alone (P < 0.05). On the other hand, more STRO-1+/CD164-DPSCs were found in the collagenase alone group with higher adipogenic potential. CONCLUSIONS:Different enzyme solutions gave rise to different populations of DPSCs. Dispase enhanced isolation of CD146+ DPSCs probably by disrupting the basement membranes of blood vessels and releasing DPCSs embedded in the perivascular niche. Furthermore, the differentiation potential of DPSCs was influenced by the change in enzyme solution.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Ebrahimi Dastgurdi M,Ejeian F,Nematollahi M,Motaghi A,Nasr-Esfahani MHdoi
10.1016/j.archoralbio.2018.05.008subject
Has Abstractpub_date
2018-09-01 00:00:00pages
74-79eissn
0003-9969issn
1879-1506pii
S0003-9969(18)30178-Xjournal_volume
93pub_type
杂志文章abstract：:One of the most popular drinks worldwide, tea is rich in polyphenols and is beneficial to our health because it contributes to the prevention of many diseases. In the human oral cavity, there are more than 750 different species of bacteria living together within dental plaque. Some of the bacteria are pathogens that c...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2019.03.027
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the factors that could participate on salivary glands hypofunction during inflammation and the participation of endocannabinoids in hyposalivation induced by the presence of inflammogens in the submandibular gland (SMG) or in the brain. DESIGN:Salivary secretion was a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.04.003
更新日期：2013-09-01 00:00:00
abstract：:A phenotypic study had shown that gingival B cells respond differently to two periodontopathic bacteria, Porphyromonas gingivalis and Fusobacterium nucleatum. Further investigation now shows a reduction in the percentage of Ki-67 + T cells in cultures of gingival and peripheral blood mononuclear cells stimulated with ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90139-y
更新日期：1992-01-01 00:00:00
abstract：:Nicotine, a major component in tobacco smoke, stimulates the synthesis of prostaglandin E(2). We investigated the involvement of the transcription nuclear factor kappa B (NFkappaB) in the nicotine-induced expression of cyclooxygenase-2 (COX-2), a key enzyme for prostaglandin synthesis, in human gingival fibroblasts. N...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.11.006
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:To determine if dentine at the enamel-dentine junction (EDJ) in man is more sensitive to hydrostatic pressure stimuli then deeper dentine. DESIGN:Cavities (1mm diam.) were cut at the tips of the buccal and lingual cusps of 8 premolars in 3 subjects (ages: 22-25 years). Both cavities were initially deepened t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.02.015
更新日期：2016-06-01 00:00:00
abstract：INTRODUCTION:Caries lesions in dental hard tissues autofluoresce when exposed to light of certain wavelengths, whereas sound tissues do not, and this can be used as an in vitro histological marker for dental caries. Detection of autofluorescence is the basis of KaVo DIAGNOdent™ technology, and provides objective feedba...
journal_title：Archives of oral biology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.archoralbio.2012.09.017
更新日期：2013-06-01 00:00:00
abstract：:Types of etching patterns and histological appearances were examined in acid-etched, NaF-treated caries-like lesions of enamel. Etching patterns similar to those for etched-sound enamel were found. Remineralization was limited to the surface zone and superficial portion of the body of the lesion following acid-etching...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90004-9
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to clarify the effects of zinc chloride (ZnCl2) exposure on the induction of heme oxygenase-1 (HO-1) expression and its regulatory mechanisms in MDPC-23 mouse odontoblast-like cells. METHODS:MDPC-23 cells were incubated with ZnCl2, and the levels of HO-1 protein, phosphorylated forms...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.09.002
更新日期：2013-04-01 00:00:00
abstract：:The fortuitous observation of salivary microliths in ferret was pursued in the present investigation. Major salivary glands obtained post-mortem from mature ferrets of either sex were examined with the use of histology and light microscopical histochemistry for calcium, protein, amino acids, mucosubstances and hydroly...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.07.009
更新日期：2006-03-01 00:00:00
abstract：:The effects of interleukin-1 (IL-1) on the metabolism of two androgenic substrates, testosterone and 4-androstenedione, that can be converted to the potent anabolic metabolite 5 alpha-dihydrotestosterone (DHT) were studied. Duplicate incubations of confluent gingival fibroblasts were made in Eagle's minimum essential ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00058-w
更新日期：1995-10-01 00:00:00
abstract：OBJECTIVE:Streptococcus mutans is a principal bacterium that forms pathogenic biofilm involved in the development of dental caries. S. mutans possesses a quorum sensing system (QS) stimulated by competence stimulating peptide (CSP), which is associated with bacteriocin production, genetic competency and biofilm formati...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.04.010
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. METHODS:Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.07.003
更新日期：2019-10-01 00:00:00
abstract：:Human epithelial cells isolated from adult gingival and infant palatal biopsies were cultured using 3T3 feeder cells. The colony-forming efficiency was about 0.8 per cent with cholera toxin and epidermal growth factor (EGF). The cell yield of cultures from infant palates depended on the concentration of cholera toxin ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90113-9
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:RhoE and regulator of G-proteins signalling (RGS) 2 were identified as the up-regulated genes in human periodontal ligament (PDL) cells under compression. RhoE belongs to the Rho GTPase family, and RGS2, a novel family of GTPase-activating proteins, turns off the G-protein signalling. Rho family proteins hav...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.11.010
更新日期：2014-02-01 00:00:00
abstract：:A task-force to resolve 26 pending forensic caseworks was carried out. We tested four different protocols to extract DNA from molar and pre-molar teeth from 26 cadavers with post-mortem intervals from 2 months to 12 years. We compared the amount of DNA and DNA profiles with the time elapsed between death and laborator...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.014
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to evaluate the performance of MALDI-TOF mass spectrometry in identifying bacteria isolated in the oral cavity known to be of probiotic interest. DESIGN:We evaluated Bruker MALDI Biotyper for the identification of 92 clinical oral isolates of probiotic interest (31 Streptococcus sali...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104983
更新日期：2021-01-01 00:00:00
abstract：:Previous laboratory studies have revealed that some axons branch to supply the pulps of two teeth, but the incidence of such fibres in different regions of the jaws has not been investigated. The present study has used electrophysiological techniques to determine the incidence and distribution of branched pulpal axons...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90109-y
更新日期：1993-11-01 00:00:00
abstract：:There may be deleterious effects if there is an age-dependent reduction in mucin composition or content in salivary glands. To assess whether there was an effect of age in submandibular glands from male mice aged 3 months, 10 and 20 months and 27, 29 and 30 months, mucin, protein and sialic acid were quantitated. Chan...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90139-l
更新日期：1991-01-01 00:00:00
abstract：:Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00038-q
更新日期：1995-08-01 00:00:00
abstract：AIMS:Anomalies of tooth number may not be isolated conditions but may have wider associations in the development of the dentition including tooth size. This study aimed to examine links between hypodontia, supernumerary teeth and crown size, considering the effect on the development of the whole dentition and so increa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.05.016
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:To clarify whether over-eruption of the mandibular third molar can disturb chewing movement. METHODS:Eighteen patients with a unilateral mandibular over-erupted third molar confirmed by both study cast observation and T-scan occlusal detection were selected from a sample of patients with complaints of tempor...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.06.012
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. DESIGN:Rats were assigned as follows (n=5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.06.006
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:The present study evaluated the nociceptive response induced by dentin hypersensitivity after dental erosion in rats that were exhibited to unpredictable chronic mild stress (UCMS)-induced depressive-like behavior. DESIGN:Adult male rats were subjected to UCMS (depression [D] group) or not (no depression [ND...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.005
更新日期：2017-08-01 00:00:00
abstract：:The mRNA for insulin-like growth factor I (IGF-I) in the submandibular gland of mature and developing rats was examined by Northern blotting and in situ hybridization with an oligonucleotide probe. In the mature adult rat, IGF-I mRNA was expressed at a higher level in the submandibular gland than in the liver, and was...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90006-8
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. DESIGN:This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.09.019
更新日期：2018-12-01 00:00:00
abstract：:Histatins are human salivary antifungal proteins that are prone to extensive enzymatic degradation upon their release into the oral cavity. Histatin proteolysis, leading to the disappearance of the intact protein can be expected to have functional consequences. Histatin 5, comprising 24 residues, is the smallest of th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.06.005
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVE:The aim of the present study was to investigate the correlation between IL-25 expression and disease severity, and the potential immunoregulatory role of IL-25 expression in oral lichen planus (OLP). MATERIALS AND METHODS:The oral mucosal tissue samples obtained from OLP patients and healthy controls (HCs) w...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：:Covalent intermolecular cross-links in collagen provide the dentine matrix with stability and tensile strength. The density of collagen cross-links varies depending on the site within the same tissue. This variation is probably due to factors such as the different amounts of stress and different turnover rates at the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90118-6
更新日期：1993-07-01 00:00:00
abstract：:The effect of a reduced functional dentition on the development of the mandibular disc in young rabbits was studied by measuring cell proliferation within the disc following tooth extraction. Maxillary and mandibular incisor teeth were extracted from 18 animals at 5 weeks of age. At 12 weeks the rabbits received 0.25 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90003-5
更新日期：1994-09-01 00:00:00
abstract：:The cartilage of the mandibular condyle of growing mice was used to monitor changes in the content of protein, DNA, hydroxyproline, hydroxylysine, uronic acid, calcium and phosphate. Protein and DNA increased in a stepwise fashion with one spurt seen during the first 2 weeks of post-natal life a second immediately aft...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90036-5
更新日期：1983-01-01 00:00:00
