Abstract:
BACKGROUND:Increasing long-term allograft survival is the main challenge in organ transplantation, and allograft loss due to chronic rejection has been found to correlate with episodes of early acute rejection. It is important to understand the mechanisms that maintain the donor-specific hyporesponsive state. This study prospectively evaluates immune events related to donor-specific hyporesponsiveness in the stable transplant patients on calcineurin inhibitors (CNIs). METHODS:Peripheral blood mononuclear cells of transplant recipients on CNI (n=19) were tested in mixed lymphocyte reaction (MLR) against donor and third-party peripheral blood mononuclear cells pretransplant and at 6 to 8 weeks, 14 to 18 weeks, and 6 to 8 months posttransplant. Interleukin (IL)-10 and transforming growth factor-beta were quantitated in cultures supernatants by enzyme-linked immunosorbent assay. CD4CD25 T-regulatory cells (Tregs) were enumerated using flow cytometry. RESULTS:All patients showed sharp decline in anti-donor and third-party MLR response at 6 to 8 weeks posttransplant with progressive decline up to 6 to 8 months. This was accompanied by increased IL-10 levels in the supernatant at all the follow-ups. Transforming growth factor-beta level in the supernatant was significantly lower at 14 to 18 weeks. Frequency of CD4CD25 Tregs showed a significant decrease at 6 to 8 weeks posttransplant, which was sustained up to 6 to 8 months. CONCLUSION:The study shows that the maintenance of good graft function in early posttransplant period in recipients on CNI is associated with a decrease in donor-specific and third-party MLRs. There is a decline in Treg numbers along with increased IL-10 levels. High IL-10, probably from a non-Tregs source, may have an important role in maintaining hyporesponsiveness and good graft function.
journal_name
Transplantationjournal_title
Transplantationauthors
Mittal SK,Sharma RK,Gupta A,Naik Sdoi
10.1097/TP.0b013e3181af20fdsubject
Has Abstractpub_date
2009-08-15 00:00:00pages
435-41issue
3eissn
0041-1337issn
1534-6080pii
00007890-200908150-00024journal_volume
88pub_type
临床试验,杂志文章相关文献
TRANSPLANTATION文献大全abstract::Since chemotaxis is an important mechanism by which leukocytes are recruited to allografts during rejection, the effect of immunosuppressant agents on in vitro chemotactic responses of neutrophils, monocytes, and lymphocytes was studied. Cyclosporine caused profound inhibition of lymphocyte chemotaxis to three differe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199011000-00020
更新日期:1990-11-01 00:00:00
abstract::In recent years there have been major advances in the technology for the detection and definition of human leukocyte antigen antibodies. In this overview we describe the evolution in laboratory technology, the techniques currently available and consider their application in antibody specificity definition and in under...
journal_title:Transplantation
pub_type: 社论,评审
doi:10.1097/TP.0b013e31817c90f5
更新日期:2008-08-15 00:00:00
abstract:BACKGROUND:Cytomegalovirus (CMV) replication has been associated with more risk for solid organ graft rejection. We wondered whether this association still holds when patients at risk receive prophylactic treatment for CMV. METHODS:We correlated CMV infection, biopsy-proven graft rejection, and graft loss in 1,414 pat...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000000160
更新日期:2014-11-15 00:00:00
abstract::Acute antibody-mediated rejection (AAR) of mouse skin allografts was studied in nine donor-recipient combinations with increasing histoincompatibility ranging from an H-Y to a complete H-2 plus on-H-2 disparity. AAR was induced by the injection of specific alloantiserum along with a heterologous complement on day 7 af...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197709000-00002
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:The timing of an acute rejection may have a variable impact on renal allograft survival. To determine whether the time of first acute transplant rejection (ATR) is an independent predictor of long-term allograft survival, we studied 31,600 first cadaveric renal transplants that were functional on the first t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199705150-00013
更新日期:1997-05-15 00:00:00
abstract:BACKGROUND:The influence of donor hematopoietic cell microchimerism on organ allograft survival has been studied largely in vascularized transplant models. Here, we examine the impact of donor bone marrow (BM) cells administered intravenously together with transient systemic tacrolimus therapy on microchimerism, the su...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199802270-00005
更新日期:1998-02-27 00:00:00
abstract:BACKGROUND:Humanized Fc receptor (FcR)-nonbinding antibodies against CD3 are promising immunosuppressive agents that may overcome both the neutralizing response to and the cytokine release syndrome seen with conventional monoclonal antibodies against CD3. In addition, evidence from several murine models suggests that t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104150-00020
更新日期:2001-04-15 00:00:00
abstract::Adhesion of leukocytes to vascular endothelium is a necessary step leading to the migration of cells into underlying tissues. Vascular adhesion molecules regulate this process and may play an important role in graft rejection. Immunocytochemical studies have been used to investigate the expression of vascular adhesion...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199209000-00013
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND:The role of natural killer (NK) cells and macrophages (Møs) in the absence of T cell immunity in discordant xenograft (xg) rejection was investigated. METHODS:Guinea pig hearts were transplanted into athymic nude rats receiving no treatment or antixenoantibody (xAb), anticomplement (C), antinatural killer (...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-15 00:00:00
abstract:BACKGROUND:Acute liver failure (ALF) carries a high mortality unless urgent orthotopic liver transplantation (OLT) is performed on time. Live donors are utilized to treat this irreversible condition first in pediatric cases and then in adults. Herein, we aimed to report our experience with live donors for ALF in a coun...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000276987.55382.e2
更新日期:2007-08-27 00:00:00
abstract::We studied major histocompatibility complex (MHC) and cytokine mRNA induction after renal injury in the absence of interferon-gamma (IFN-gamma) using IFN-gamma gene knockout (GKO) mice. The left renal pedicle of normal (wild-type) and GKO BALB/c mice was clamped for 60 minutes; cytokine and MHC mRNA expression were mo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199560120-00031
更新日期:1995-12-27 00:00:00
abstract::Rat recipients of renal allografts and unilaterally nephrectomized control rats were studied to evaluate the response in blood pressure to prednisolone in diverse doses, and to determine the dosage required to achieve adequate immunosuppression without undue complication of hypertension. While a continuous infusion of...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198703000-00007
更新日期:1987-03-01 00:00:00
abstract::MHC expression on endothelial cells after cytomegalovirus infection was investigated using double antibody radioimmunoassays and immunofluorescence flow cytometry. After CMV infection there was an increase in class I and beta 2 microglobulin expression. This increase corresponds to the percentage of cells that are inf...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198909000-00024
更新日期:1989-09-01 00:00:00
abstract::This study focuses upon two discrete components of posttransplant hepatic reticuloendothelial system (RES) function-phagocytosis and killing of bacteria-under various conditions of ischemic preservation. We had previously reported that, following intravenous injection of rats with 51Cr and 125I double-labeled Escheric...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199604270-00006
更新日期:1996-04-27 00:00:00
abstract:BACKGROUND:Donor pigs transgenic for human decay-accelerating factor (hDAF) were used in a xenogeneic ex vivo liver perfusion model to study the effect of this modification on the development of hyperacute rejection. METHODS:Three transgenic pigs were hepatectomized after hypothermic portal and transaortal gravity per...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199708150-00002
更新日期:1997-08-15 00:00:00
abstract::The aim of this study was to assess the efficacy of an enteral diet fortified with arginine, RNA, and fish oil (Impact), alone and in combination with cyclosporine (CsA) and donor-specific transfusion (DST) on allograft survival in the ACI:Lewis rat cardiac transplant model. Animals were fed ad libitum with either sta...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-10-27 00:00:00
abstract::P-31 nuclear magnetic resonance (NMR) spectroscopy was used to evaluate heterotropic cardiac allograft rejection in the rat. To enable optimal application of NMR spectroscopy for the in vivo study of myocardial metabolism in the transplanted organ, an accessory heart transplantation to groin in the rat was used. The a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198708000-00002
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND:CTLA4 immunoglobulin (Ig)G that binds to B7 effectively inhibits the signaling of CD28/CTLA4-B7 pathway and induces antigen specific T cell unresponsiveness in vitro and in vivo. Using CTLA4IgG, we examined induction of long-term graft survival and the mechanism of maintenance of tolerance in rat allogeneic ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199902270-00005
更新日期:1999-02-27 00:00:00
abstract::Publicly available program-specific data from the scientific registry of transplant recipients were used to determine the association between adult lung transplant center volume and 1-year recipient mortality from 2000 to 2007. We found a significant inverse association between the center volume of adult lung transpla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181ceecf7
更新日期:2010-03-27 00:00:00
abstract::In order to study further whether a relationship exists between the extent of ischemia-preservation-reperfusion injury (IPRI) and acute rejection (AR) events in liver allografts, we retrospectively reviewed 213 consecutive cyclosporine-treated patients who received their first liver allograft between 1/1/93 and 12/31/...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199509270-00006
更新日期:1995-09-27 00:00:00
abstract::Mice immunized with allogeneic cells exhibit a local component of cellular immunity that is confined to lymph nodes draining the site of immunization. After immunization of C3H mice by skin grafting or by i.p. or footpad injection of F1 hybrid spleen cells, draining lymph nodes show increased responsiveness toward the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198107000-00006
更新日期:1981-07-01 00:00:00
abstract:BACKGROUND:Deciphering the mechanisms of tolerance and chronic rejection (CR) remains a major goal in transplantation. Data in rodents suggest that Toll-like receptors (TLR), regulators of innate immune responses, play a role in determining graft outcome. However, few studies have focused on TLR expression in human kid...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31817b8dc5
更新日期:2008-07-15 00:00:00
abstract:BACKGROUND:Islet transplantation studies with diabetic rodents frequently use treatment with diabetogens such as alloxan or streptozotocin to render hosts hyperglycemic. These chemicals produce unwanted toxic side effects, which complicate interpretations of damage produced by hyperglycemia versus direct toxin-induced ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200204270-00024
更新日期:2002-04-27 00:00:00
abstract::This study evaluated the utility of duplex Doppler sonograms (DS) and the resistive index (RI) in the identification and differential diagnosis of various causes of renal allograft dysfunction. The efficacy of DS and RI was studied either during acute episodes of allograft dysfunction or during periodic posttransplant...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199012000-00015
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND:The impact of community-acquired respiratory virus (CARV) infections on bronchiolitis obliterans syndrome (BOS) and outcome after lung transplantation (LTx) and diagnostic techniques were prospectively evaluated. METHODS:A single-center prospective cohort study was performed in LTx-outpatients between Octob...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181a4857d
更新日期:2009-05-27 00:00:00
abstract::Patient entry is now complete in a prospective trial of anti-Tac, a murine IgG2a monoclonal antibody directed against the p55 chain of the human IL-2 receptor, for the prevention of renal allograft rejection. Recipients of primary cadaver allografts were randomized to receive either anti-Tac (20 mg q.d. x 10 days begi...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199101000-00016
更新日期:1991-01-01 00:00:00
abstract::In a canine model of acute ischemic lung injury, a hypertonic citrate solution (HTC) widely used for renal preservation in the United Kingdom, was compared with modified Euro-Collins' solution (ECS) currently the most widely clinically used pulmonary perfusate. Ten beagle dogs underwent left thoracotomy and exclusion ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199105000-00022
更新日期:1991-05-01 00:00:00
abstract::Using C1q binding, Kg binding, and Raji cell radioimmunoassays, the relationship between renal allograft rejection and the circulating immune complexes was examined in 90 patients with renal transplants. The data showed that there was a significant quantitative correlation between C1q-binding and Raji-cell-binding act...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198308000-00009
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:Successful pancreatic islet transplantation (PIT) has resulted in several transplant centers wanting to start PIT programs. PIT remains experimental and must be performed safely for its continued use. We describe the radiographic techniques used at our center and their results. METHODS:Between January 17, 2...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000073976.26604.96
更新日期:2003-07-15 00:00:00
abstract::Cultured BALB/c (H-2d) thyroid, adult pancreatic islet and fetal proislet tissues can be accepted permanently in CBA/H (H-2k) recipients until rejection is triggered by the transfer of antidonor strain activated immune T cells. Class II MHC antigens are not expressed on the accepted grafts and are induced following im...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199204000-00025
更新日期:1992-04-01 00:00:00