Donor bone marrow potentiates the effect of tacrolimus on nonvascularized heart allograft survival: association with microchimerism and growth of donor dendritic cell progenitors from recipient bone marrow.

Abstract:

BACKGROUND:The influence of donor hematopoietic cell microchimerism on organ allograft survival has been studied largely in vascularized transplant models. Here, we examine the impact of donor bone marrow (BM) cells administered intravenously together with transient systemic tacrolimus therapy on microchimerism, the survival of nonvascularized cardiac allografts, and growth of donor antigen-presenting cells [dendritic cells (DCs)] from recipient BM. METHODS:Adult male C3H (H2k) mice received heterotopic heart transplants from B10 (H2b) donors in the dorsal ear pinna. They were given no further treatment, or either a short course of tacrolimus (FK506; 2 mg/kg i.p. from day 0 to day 13), unmodified donor BM cells (50x10(6) i.v. on day 0) or both treatments. Grafts were examined daily for contractile activity. Anti-donor cytotoxic T lymphocyte responses were determined in recipients' spleens. Microchimerism (IAb+ cells) was demonstrated by immunocytochemical staining of spleens, and of cells expanded from recipient BM using cytokines and culture conditions that promote the growth of DCs. RESULTS:Tacrolimus alone significantly prolonged median heart graft survival time from 10 to 22 days (P<0.001). BM alone failed to prolong graft survival. By contrast, tacrolimus + donor BM resulted in a mean survival time of 42 days (P<0.01 compared with tacrolimus treatment alone). This marked increase in heart allograft survival was associated with reduced anti-donor cytotoxic T lymphocyte responses attributable to a nonspecific effect of tacrolimus. In addition, however, a link was observed between the beneficial effect of donor BM and comparatively large numbers of donor major histocompatibility complex class II (IAb+)-positive cells in recipients' spleens, and in cultures of granulocyte-macrophage colony-stimulating factor + interleukin-4-stimulated DCs from recipients' BM. No donor-derived cells were propagated from heart graft recipients given either tacrolimus or donor BM alone. CONCLUSIONS:This nonvascularized organ transplant model demonstrates the positive effect on allograft survival of donor BM given at the time of transplant to transiently immunosuppressed recipients. The findings also reveal links between hematopoietic cell chimerism, the presence of donor DC progenitors in recipient BM, and organ allograft survival.

journal_name

Transplantation

journal_title

Transplantation

authors

Khanna A,Steptoe RJ,Antonysamy MA,Li W,Thomson AW

doi

10.1097/00007890-199802270-00005

subject

Has Abstract

pub_date

1998-02-27 00:00:00

pages

479-85

issue

4

eissn

0041-1337

issn

1534-6080

journal_volume

65

pub_type

杂志文章
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    abstract:BACKGROUND:An increase in serum alpha-glutathione S-transferase concentration (GST) has been shown to be a more sensitive and specific marker of hepatocellular damage than equivalent increases in transaminase activities. A randomized clinical trial of 60 liver transplants in 49 patients was carried out to assess the cl...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00007890-199711270-00013

    authors: Hughes VF,Trull AK,Gimson A,Friend PJ,Jamieson N,Duncan A,Wight DG,Prevost AT,Alexander GJ

    更新日期:1997-11-27 00:00:00

  • Failure of reactivation of hepatitis B after liver transplantation in hepatitis B surface antigen-negative, core antibody-positive recipients.

    abstract:BACKGROUND:Hepatitis B virus (HBV) infection after liver transplantation may occur in patients previously not exposed to the virus, but who receive an organ from a surface antigen (HBsAg)-negative, core antibody (HBcAb)-positive donor. The risk of HBV reactivation after liver transplantation in recipients that are HBsA...

    journal_title:Transplantation

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    doi:10.1097/00007890-200202150-00027

    authors: Maguire D,Heaton ND,Smith HM

    更新日期:2002-02-15 00:00:00

  • Tendinitis--a common complication after renal transplantation.

    abstract::A group of 170 patients having functional renal allografts were evaluated at least six months after renal transplantation. They were assessed for symptoms and signs of tendinitis in the Achilles and Supraspinatus tendons. Of the 170 patients, 34 had tendinitis. Four patients (11.8%) had spontaneous rupture of the Achi...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Murison MS,Eardley I,Slapak M

    更新日期:1989-10-01 00:00:00

  • Lectin-dependent cell-mediated cytotoxicity and natural killer function in rejecting and infected lung allografts.

    abstract::Differentiation between rejection and infection of lung allografts remains difficult. The effects of these two pathologic entities on the cytolytic activity of bronchoalveolar lavage (BAL) and PBL were investigated. Left lung allotransplantation was performed on 16 mongrel dogs of which 12 were available for complete ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199306000-00008

    authors: Nguyen DM,Mulder DS,Shennib H

    更新日期:1993-06-01 00:00:00

  • Renal transplantation without sutures using the vascular clipping system for renal artery and vein anastomosis--a new technique.

    abstract::A cadaveric renal transplant was performed on a 63-year-old woman. The donor renal artery and vein were anastomosed to the recipient external iliac vessels using the vascular clipping system. These vascular anastomoses were performed with four stay sutures and several clips for each anastomosis, without a continuous v...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199610270-00024

    authors: Mital D,Foster PF,Jensik SC,del Rio JV,Sankary HN,McChesney LP,Williams JW

    更新日期:1996-10-27 00:00:00

  • Corneal Stromal Transplantation With Human-derived Acellular Dermal Matrix for Pellucid Marginal Corneal Degeneration: A Nonrandomized Clinical Trial.

    abstract:BACKGROUND:To investigate the transparency, biocompatibility, and safety of human-derived acellular dermal matrix for application in corneal stromal transplantation. METHODS:Twenty-four patients (24 eyes) with pellucid marginal corneal degeneration were enrolled, and intrastromal keratoplasty was performed with human-...

    journal_title:Transplantation

    pub_type: 临床试验,杂志文章

    doi:10.1097/TP.0000000000002681

    authors: Jiang X,Wang Y,Qiu W,Huang C,Liu Z,Ding T,Shi D,Li X

    更新日期:2019-06-01 00:00:00

  • Comparison of human islet isolation outcomes using a new mammalian tissue-free enzyme versus collagenase NB-1.

    abstract:BACKGROUND:After the discontinuation of the manufacturing Liberase HI because of a small potential for prion disease transmission, Roche Diagnostics (Indianapolis, IN) developed a new enzyme product (Liberase MTF [mammalian tissue free]), which is similar to Liberase HI with the exception that no mammalian tissue is us...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181e117ce

    authors: O'Gorman D,Kin T,Imes S,Pawlick R,Senior P,Shapiro AM

    更新日期:2010-08-15 00:00:00

  • Effect of sirolimus on the metabolism of apoB100- containing lipoproteins in renal transplant patients.

    abstract:BACKGROUND:Sirolimus (Rapamune, rapamycin, RAPA) is a potent immunosuppressive drug that has reduced the rate of acute rejection episodes by more than 40% in phase III trials when added to an immunosuppression regimen of cyclosporine (CsA) and prednisone. However, RAPA treatment tends to increase lipid levels, particul...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200110150-00011

    authors: Hoogeveen RC,Ballantyne CM,Pownall HJ,Opekun AR,Hachey DL,Jaffe JS,Oppermann S,Kahan BD,Morrisett JD

    更新日期:2001-10-15 00:00:00

  • The effect of renal ischemia on cyclosporine clearance in rabbits.

    abstract::The renal handling of cyclosporine was studied in ischemically damaged kidneys in New Zealand White rabbits and nonischemic control animals. CsA, 25 mg/kg/day, was administered intravenously for 10 days starting with the day of operation. Blood CsA (B CsA) was higher in the ischemic group compared with the controls (m...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199003000-00004

    authors: Karim MS,Wood RF,Dawnay AB,Fulton PA

    更新日期:1990-03-01 00:00:00

  • Hepatitis C infection, time in renal-replacement therapy, and outcome after kidney transplantation.

    abstract:BACKGROUND:Hepatitis C virus (HCV) infection is common in kidney transplantation and is known to affect long-term patient and graft survival, as is time in renal-replacement therapy (RRT). The aim of this study was to investigate HCV in relation to time in RRT and its impact on outcome after transplantation. METHODS:A...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000131948.29742.24

    authors: Bruchfeld A,Wilczek H,Elinder CG

    更新日期:2004-09-15 00:00:00

  • Antibody testing strategies for deceased donor kidney transplantation after immunomodulatory therapy.

    abstract:BACKGROUND:Immunomodulatory protocols including intravenous immunoglobulin/rituximab (IVIG/R) are employed to decrease anti-human leukocyte antigen (HLA) antibody levels for patients broadly sensitized to HLA and increase chances for transplantation with a compatible deceased donor (DD). The aim of our study was to ide...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e31821eab8a

    authors: Lai CH,Cao K,Ong G,Naim M,Wang Q,Mirocha J,Vo A,Jordan SC,Reinsmoen NL

    更新日期:2011-07-15 00:00:00

  • IgG antibodies against an HLA antigen are associated with activated cytotoxic T cells against this antigen, IgM are not.

    abstract::Pre-existing alloantibodies against the mismatched HLA-A and HLA-B antigens of the donor, when present in current sera, are believed to be detrimental to kidney graft survival. When these antibodies are present only in historical sera, their immunoglobulin class has reported to be important with respect to the expecte...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199405150-00018

    authors: Roelen DL,van Bree J,Witvliet MD,Habraken I,van Beelen E,van't Sant PH,de Waal LP,van Rood JJ,Claas FH

    更新日期:1994-05-15 00:00:00

  • 32 Doses of Bortezomib for Desensitization Is Not Well Tolerated and Is Associated With Only Modest Reductions in Anti-HLA Antibody.

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    journal_title:Transplantation

    pub_type: 临床试验,杂志文章

    doi:10.1097/TP.0000000000001330

    authors: Moreno Gonzales MA,Gandhi MJ,Schinstock CA,Moore NA,Smith BH,Braaten NY,Stegall MD

    更新日期:2017-06-01 00:00:00

  • Role of anti-tumor necrosis factor-alpha in ischemia/reperfusion injury in isolated rat liver in a blood-free environment.

    abstract:BACKGROUND:Warm ischemia/reperfusion injury during liver transplantation is the most important cause of primary nonfunction of liver allografts. Tumor-necrosis factor (TNF)-alpha apparently mediates tissue damage by inducing apoptosis and/or necrosis in liver transplants. The aim of the study was to determine, using an...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200206270-00004

    authors: Ben-Ari Z,Hochhauser E,Burstein I,Papo O,Kaganovsky E,Krasnov T,Vamichkim A,Vidne BA

    更新日期:2002-06-27 00:00:00

  • Endovascular treatment of hepatic venous outflow obstruction after piggyback technique liver transplantation.

    abstract:BACKGROUND:The piggyback technique of orthotopic liver transplantation is an attractive alternative that preserves the recipient inferior vena cava and allows uninterrupted venous blood return during the anhepatic phase. As with other transplantation techniques, the vascular anastomoses required by the piggyback techni...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199908150-00018

    authors: Sze DY,Semba CP,Razavi MK,Kee ST,Dake MD

    更新日期:1999-08-15 00:00:00

  • Molecular Imaging of Acute Cardiac Transplant Rejection: Animal Experiments and Prospects.

    abstract::Acute rejection (AR) remains the biggest challenge during the first year after heart transplantation despite advances in immunosuppressive therapy. The early detection and curbing of AR are crucial to the survival of transplant recipients. However, as the criterion standard for AR, endomyocardial biopsy has several li...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/TP.0000000000001780

    authors: Chen Y,Zhang L,Liu J,Zhang P,Chen X,Xie M

    更新日期:2017-09-01 00:00:00

  • Decrease in 1-year Kidney Graft Size Predicts Inferior Outcomes After Deceased Donor Kidney Transplantation.

    abstract:BACKGROUND:Longest bipolar length of the kidney graft is routinely measured for ultrasonographic assessment of graft size (GS), although the value of the graft length remains unclear. METHODS:In a single-center, observational study involving 319 deceased-donor kidney transplant recipients, we assessed variations in ab...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000000977

    authors: Černe S,Arnol M,Kandus A,Buturović-Ponikvar J

    更新日期:2016-08-01 00:00:00

  • Non-HLA immunogenetic polymorphisms and the risk of complications after allogeneic hemopoietic stem-cell transplantation.

    abstract:BACKGROUND:Existing data indicate that non-human leukocyte antigen (HLA) immunogenetic polymorphisms influence the risk of complications after allogeneic hemopoietic stem-cell transplantation. However, prior studies have been limited by small sample size and limited genotyping. METHODS:We examined 22 polymorphisms in ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000111769.45088.a2

    authors: Mullighan C,Heatley S,Doherty K,Szabo F,Grigg A,Hughes T,Schwarer A,Szer J,Tait B,To B,Bardy P

    更新日期:2004-02-27 00:00:00

  • Dog leukocyte antigen-haploidentical stem cell allografts after anti-CD44 therapy and nonmyeloablative conditioning in a preclinical canine model.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000228908.10775.b0

    authors: Fukuda T,Kerbauy FR,Gooley T,Santos EB,Storb R,Sandmaier BM

    更新日期:2006-08-15 00:00:00

  • Neutrophilic tubulitis as a marker for urinary tract infection in renal allograft biopsies with C4d deposition.

    abstract:BACKGROUND:Neutrophilic tubulitis accompanied by intratubular neutrophil clusters in the renal allograft is a surrogate marker for urinary tract infection (UTI). Overlapping histologic findings can occur in antibody-mediated rejection, which is characterized by peritubular capillary (PTC) deposition of C4d. This study ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e31819ca304

    authors: Gupta G,Shapiro R,Girnita A,Batal I,McCauley J,Basu A,Tan H,Randhawa P

    更新日期:2009-04-15 00:00:00

  • Atypical mixed lymphocyte culture. Identity between mother and child in leukemia and aplastic anemia.

    abstract::The mixed lymphocyte culture tests between one patient with acute lymphocytic leukemia and her mother and between another patient with aplastic anemia and his mother were found nonreactive, despite a one-haplotype difference for HL-A. Two crossovers in each family at the MLC locus or a specific defect in lymphocyte r...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-197501000-00008

    authors: Opelz G

    更新日期:1975-01-01 00:00:00

  • Analysis of factors affecting recurrence of hepatocellular carcinoma after liver transplantation with a special focus on inflammation markers.

    abstract:BACKGROUND:Systemic inflammation markers, such as neutrophil-to-lymphocyte ratio (NLR), have recently emerged as the prognostic factors for recurrence of liver tumors. METHODS:We assessed the ability of NLR and of other variables to predict the outcomes of orthotopic liver transplantation (OLT) for hepatocellular carc...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3182187cf0

    authors: Bertuzzo VR,Cescon M,Ravaioli M,Grazi GL,Ercolani G,Del Gaudio M,Cucchetti A,D'Errico-Grigioni A,Golfieri R,Pinna AD

    更新日期:2011-06-15 00:00:00

  • Recurrence of focal-segmental glomerulosclerosis in children after renal transplantation: clinical and genetic aspects.

    abstract::Focal segmental glomerulosclerosis (FSGS) is the primary renal disease in approximately one-tenth of pediatric patients receiving a renal allograft. Recurrence of proteinuria after renal transplantation is observed in approximately 30% of patients and negatively impacts graft survival. Risk factors for recurrence are ...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/01.tp.0000187110.25512.82

    authors: Weber S,Tönshoff B

    更新日期:2005-09-27 00:00:00

  • Subclinical Antibody-mediated Rejection After Kidney Transplantation: Treatment Outcomes.

    abstract:BACKGROUND:Antibody-mediated rejection (AMR) is a leading cause of morbidity and mortality after kidney transplantation. Early diagnosis and treatment of subclinical AMR based on the donor-specific antibody (DSA) testing may result in better outcomes. METHODS:We tested this hypothesis in 220 kidney transplant recipien...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000002566

    authors: Parajuli S,Joachim E,Alagusundaramoorthy S,Blazel J,Aziz F,Garg N,Muth B,Mohamed M,Mandelbrot D,Zhong W,Djamali A

    更新日期:2019-08-01 00:00:00

  • Studies on the immunosuppressive properties of cyclosporin a in rats receiving renal allografts.

    abstract::The immunosuppressive effects of cyclosporin A were tested in a DA (RT-1a) to Lewis (RT-1(1) rat renal allograft model, which represents a very strong histocompatibility barrier. Dose-response studies established that oral doses of 5 mg/kg/day or higher gave complete suppression of rejection, while oral doses of 2 mg/...

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    pub_type: 杂志文章

    doi:10.1097/00007890-198005000-00003

    authors: Homan WP,Fabre JW,Williams KA,Millard PR,Morris PJ

    更新日期:1980-05-01 00:00:00

  • Immunoblastic lymphoma of donor origin in the allograft after lung transplantation.

    abstract::Posttransplant lymphoproliferative disorders (PTLD) are EBV-associated lymphoid neoplasms that are caused by the uncontrolled growth of EBV-infected B lymphocytes. The clinical presentation of PTLD can range from benign polygonal lymphoproliferative disorders to aggressive monoclonal immunoblastic lymphomas. In this r...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199606270-00010

    authors: Mentzer SJ,Longtine J,Fingeroth J,Reilly JJ,DeCamp MM,O'Donnell W,Swanson SJ,Faller DV,Sugarbaker DJ

    更新日期:1996-06-27 00:00:00

  • Interaction of mycophenolate mofetil and HLA matching on renal allograft survival.

    abstract:INTRODUCTION:The importance of HLA matching for renal transplantation outcomes has been appreciated for several decades. It has been hypothesized that as pharmacologic immunosuppression becomes stronger and more specific, the impact of HLA matching may be vanishing. Mycophenolate Mofetil (MMF) has been demonstrated to ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200102150-00010

    authors: Meier-Kriesche HU,Ojo AO,Leichtman AB,Magee JC,Rudich SM,Hanson JA,Cibrik DM,Kaplan B

    更新日期:2001-02-15 00:00:00

  • Risk factors for liver transplantation across the ABO barrier.

    abstract::A retrospective study was performed to identify risk factors affecting the fate of liver allografts transplanted across the ABO barrier. Over a 5-year period, 30 patients received 31 emergency OLTs with ABO-incompatible allografts. One-year graft and patient survival rates were 37.1% and 48.4%, respectively. This grou...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199409150-00003

    authors: Lo CM,Shaked A,Busuttil RW

    更新日期:1994-09-15 00:00:00

  • Endotelin ETA and ETB receptor antagonism during cold preservation in renal transplantation.

    abstract:BACKGROUND:In this study, we investigated the effects of selective endothelin (ET) receptor antagonism during different periods of cold ischemia on glomerular and tubular function and long-term survival in renal transplantation. METHODS:Left renal transplantation was performed in Lewis rats after 2 hr of cold ischemia...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200103150-00007

    authors: Hammad FT,Davis G,Zhang XY,Wheatley AM

    更新日期:2001-03-15 00:00:00

  • Sirolimus-based therapy for kidney transplantation from expanded criteria donors.

    abstract::The new class of immunosuppressants--inhibitors of the mammalian target of rapamycin--has no nephrotoxicity and the capacity to inhibit vascular smooth cell proliferation. These characteristics may afford considerable clinical advantages in the transplantation of kidneys from expanded criteria donors (ECD). Six clinic...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181a058a1

    authors: Rigotti P,Kahan BD

    更新日期:2009-04-27 00:00:00