Abstract：
:Programmed death-ligand 1 (PD-L1) is an inhibitory transmembrane protein that can prevent autoimmune response. Upregulated PD-L1 serves as a predictive biomarker for patients who may respond well to immune checkpoint therapies. However, variable associations of PD-L1 level with prognoses have been reported. In this study, a short peptide sequence corresponding to PD-L1 amino acids 172-187 (from the extracellular Ig-like C-type domain, and with high predicted antigenicity and hydrophilicity) was used to generate a monoclonal antibody (mAb). The resultant PD-L1 mAb, clone HC16, was examined for binding specificity and reactivity in cancer cell-lines, as assessed by immunocytochemical, immunoblotting, and co-immunoprecipitation. The potential diagnostic and clinical applicability of clone HC16 was further tested using malignant tissue arrays derived from various cancer types analyzed with an automated immunohistochemical (IHC) staining platform. Additionally, tumor samples from patients diagnosed with non-small cell lung cancer (NSCLC) were analyzed by western blotting. Clone HC16 showed obvious staining activity in lung and breast cancer tissues. Interestingly, we observed that PD-L1 level was negatively associated with clinical stage in NSCLC. Strong PD-L1 expression tended to be found in patients diagnosed with bronchioloalveolar carcinoma (BAC). These results demonstrate that clone HC16 harbors good target specificity and is suitable for further development in diagnostic tools to assess PD-L1 expression in human tissues. In addition, our findings also suggest a role for PD-L1 in a non-invasive subtype of lung cancer.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Su BC,Ting CH,Lee KY,Wu SM,Feng PH,Chan YF,Chen JYdoi
10.14670/HH-18-272subject
Has Abstractpub_date
2020-10-28 00:00:00pages
18272eissn
0213-3911issn
1699-5848pii
HH-18-272pub_type
杂志文章abstract：:In the present study, morphological changes of the exocrine pancreas in rats after pancreatic duct ligation were examined with light microscopy (hematoxylin-eosin, TUNEL, and PCNA staining) and scanning electron microscopy in order to elucidate the effects of increased pancreatic duct pressure. On the fifth day after ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-17.1033
更新日期：2002-10-01 00:00:00
abstract：:Emperipolesis has recently been described as a constant feature of "biphasic squamoid" papillary renal cell carcinoma (BPRCC). We also noticed this in some high-grade (HG) RCC, which promoted the present study to estimate the incidence of emperipolesis in RCCs and to describe them in further detail. 14 cases of HGRCC ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-925
更新日期：2018-03-01 00:00:00
abstract：:Aurora A and Aurora B are serine-threonine kinase proteins which have both been implicated in human carcinogenesis through development of aneuploidy and chromosomal instability. The aim of the study is to assess the correlation of both markers with clinical and pathological parameters in patients with bladder cancer o...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-25.1371
更新日期：2010-11-01 00:00:00
abstract：:The aim of our study was to evaluate corneal cell proliferation and apoptosis in cases of granular, macular and lattice dystrophy, and to provide evidence which may help to clarify whether apoptosis is a pathogenic factor in any of these dystrophies. The study group comprised 39 eyes (from 33 patients) which had under...
journal_title：Histology and histopathology
pub_type： 杂志文章,多中心研究
doi：10.14670/HH-22.837
更新日期：2007-08-01 00:00:00
abstract：:The hindgut is the major site in the horse for nutrient digestion and absorption. Most of this activity occurs in the large intestinal compartments, i.e., cecum, right and left ventral colon and left and right dorsal colon. The colonic pelvic flexure is a short and narrow loop connecting the left ventral and left dors...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.983
更新日期：2000-07-01 00:00:00
abstract：:Thyroid glands from 7 marmosets (Callithrix jacchus) of different age groups (newborn, 2 weeks, 2, 7, 8 and 11 months, and 8 years) were grown as organ culture according to Trowell at the medium/air interphase for 3 to 30 days. The morphology of thyroid tissue was well preserved until the end of the culture period. Ne...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:An ultrastructural and histological study was made to analyse the structural and cellular features of the pulmonary lesions produced in Wistar rats by intraperitoneal (i.p.) administration of cyclophosphamide (two i.p. doses of 150 mg CP/1 kg bw/1 ml PBS). Rats exposed to cyclophosphamide (CP) developed a condition wh...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-14.1145
更新日期：1999-10-01 00:00:00
abstract：:One hundred and eleven pituitary glands of patients (93 males, 18 females; mean age 32 years, 5 months) who died of fully developed AIDS or ARC were examined under light microscopy with the aid of immunohistochemistry. On post mortem (p.m.) examination a wide series of multiorgan alterations was noticed. Microscopical...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Chorioamnionitis and preeclampsia account for the majority of preterm births worldwide. Thus far, adequate methods for early detection or prevention of these diseases are lacking. In preeclampsia, accelerated villous maturation is believed to compensate placental insufficiency. However, little is known about the effec...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18-205
更新日期：2020-08-01 00:00:00
abstract：:Fibrosis is a pathological feature of most chronic diseases and leads to the dysfunction of various organs. However, there is currently no effective method for treating fibrosis. In recent years, a small gas, sulfur dioxide (SO₂), which can be generated endogenously in mammals, has been found to have vasorelaxation ac...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18-169
更新日期：2019-12-01 00:00:00
abstract：:Cell secretion is an important physiological process that ensures smooth metabolic activities, tissue repair and growth and immunological functions in the body. It occurs when the intracellular secretory materials are released to the exterior; these may be in the form of lipids, protein or mucous and may travel throug...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18.597
更新日期：2003-04-01 00:00:00
abstract：:Glucose uptake and metabolism are essential for proliferation and survival of cells, and are supposed to be enhanced in actively proliferating cell systems such as embryonic and cancer tissues. Glucose uptake is usually carried out through glucose transporters. In the developing fetal lung, metabolism of glucose is th...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-14.895
更新日期：1999-07-01 00:00:00
abstract：:A current concern about the biological effects of electromagnetic fields (EMF) is increasing with the wide spread use of X-band microwaves (MW, 8-10 GHz range). Gigahertz transverse electromagnetic (GTEM) field flat transmission lines are currently being used for experimental exposure of biological samples to high fre...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.1187
更新日期：2011-09-01 00:00:00
abstract：:Platelet-collagen interaction is a complex event that involves ligand-receptor interaction. There are many adhesive non-integrin receptors for platelets to interact with various types of collagens. These non-integrin receptors also serve as signal transducers both from the outside of platelets to the inside and possib...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-14.579
更新日期：1999-04-01 00:00:00
abstract：:Melanopsin-expressing retinal ganglion cells (mRGCs) constitute a system in the mammalian retina used for irradiance detection, regulating non-image forming functions, such as photoentrainment of circadian rhythms, control of the pupillary light reflex, masking response, light-regulated melatonin secretion, and modula...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-18-138
更新日期：2019-12-01 00:00:00
abstract：:Pulmonary fibrosis is a common response to various injuries to the lung. The resolution of a fibroproliferative response after lung injury is key to survival. Although there are various initiating factors or causes, the terminal stages are characterized by proliferation and progressive accumulation of connective tissu...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-19.867
更新日期：2004-07-01 00:00:00
abstract：:Our study aimed to investigate the association of Pyruvate Kinase isozyme type M2 (PKM2) with radiation resistance in locally advanced cervical squamous cell carcinoma (LACSCC). We retrospectively reviewed 132 female patients who received primary radiation therapy to treat LACSCC at Federation Internationale of Gyneco...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-627
更新日期：2015-11-01 00:00:00
abstract：:Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basal layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the reg...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:In recent years, the different mechanisms of epithelial cell loss which occur in mammalian and avian small intestine have been re-investigated. Information is now available for a variety of mammalian types and mechanisms can be divided into two major classes: [i] those preserving epithelial integrity by maintaining in...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-14.257
更新日期：1999-01-01 00:00:00
abstract：:Systematic studies on hepatic stellate cells and myofibroblasts have so far mainly focused on cells located in the perisinusoidal space of Disse, the so-called littoral compartment. Here, these cells play a key role for intralobular fibrogenesis and sinusoidal capillarization. However, advanced hepatic fibrosis and ci...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-14.719
更新日期：1999-07-01 00:00:00
abstract：:Nitric oxide (NO) is a widely recognized mediator of physiological and pathophysiological signal transmission. Its generation through L-arginine metabolism is relevant in the mesangium of the kidney where NO is produced by constitutive and inducible NO-synthase isoenzymes. Signaling is achieved through target interact...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.1151
更新日期：2000-10-01 00:00:00
abstract：:In the present work we analyze the cerebellum of chronic parkinsonian monkeys in order to clarify whether chronic mesencephalic depletion is associated with long term activation of the cerebellar neurons in chronic Parkinsonism. In our study, we observed a persistent activation of Purkinje cells in the cerebellum of c...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-27.89
更新日期：2012-01-01 00:00:00
abstract：:Smads are signal transducers for the members of the TGF-beta superfamily. Of these Smads, Smad4 is essential for TGF-beta signaling. The purpose of this study was to elucidate Smad4 expression and localization in 65 gastric adenomas, 49 intestinal-type and 39 diffuse type of gastric adenocarcinomas (including 12 cases...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-20.543
更新日期：2005-04-01 00:00:00
abstract：:To understand the role of microtubules and microfilaments in regulating endothelial monolayer integrity and repair, and since microtubules and microfilaments show some co-alignment in endothelial cells, we tested the hypothesis that microtubules organize microfilament distribution. Disruption of microtubules with colc...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-20.455
更新日期：2005-04-01 00:00:00
abstract：:The main objective of this ultrastructural study was to gain a better understanding of the involvement of brain vasculature in clearance of proteins from edematous fluid. For this purpose, both native and modified (cationized, glucosylated, and mannosylated) bovine serum albumin-gold complexes (BSA-G, catBSA-G, glucBS...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-13.647
更新日期：1998-07-01 00:00:00
abstract：:Orexins A and B are two hypothalamic peptides, involved in the central regulation of feeding, which act through two receptor subtypes, named OX1R and OX2R. OX1R is selective for orexin-A, and OX2R binds both orexins. We have investigated the effects of three subcutaneous injections of 10 nmol/kg body weight of orexins...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-16.713
更新日期：2001-07-01 00:00:00
abstract：:Gp96 is the endoplasmic reticulum (ER)-resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furt...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-28.61
更新日期：2013-01-01 00:00:00
abstract：:Amyloidosis is a group of diseases characterized by the extracellular deposition of protein that contains non-branching, straight fibrils on electron microscopy (amyloid fibrils) that have a high content of beta-pleated sheet conformation. Various biochemically distinct proteins can undergo transformation into amyloid...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-20.1295
更新日期：2005-10-01 00:00:00
abstract：:Dysadherin, a cancer associated cell membrane glycoprotein, has been reported to downregulate E-cadherin. Aberrant expression of E-cadherin has been associated with the development of metastases in patients with cancer. Even though the expression of dysadherin and E-cadherin has been studied in primary non-small cell ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-25.1257
更新日期：2010-10-01 00:00:00
abstract：:Transforming growth factor-betas (TGF-betas) are multifunctional cytokines that exist in 3 isoforms in mammals. The TGF-betas are ubiquitously expressed and all isoforms are secreted as biologically inactive precursors called latent TGF-beta (L-TGF-beta). L-TGF-betas are generally not effective molecules because they ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-16.541
更新日期：2001-04-01 00:00:00
