Description of a hybridoma bank towards Bordetella pertussis toxin and surface antigens.

Abstract:

:This paper describes the development of a murine bank of monoclonal antibodies against Bordetella pertussis toxin, filamentous hemagglutinin (FHA), pili, lipopolysaccharide (LPS), or outer membrane proteins (OMPs). Subunits S1, S2, S3 of pertussis toxin (PT) bound immunoglobulins and glycoproteins such as fetuin and haptoglobin in an unspecific manner. The specificity of monoclonal antibodies towards subunits S1, S2, S3 or S4 of PT could be demonstrated by using purified immunoglobulins or their Fab2 fragments. A set of FHA-specific monoclonal antibodies could be differentiated on the basis of their binding to the various breakdown products present in FHA preparations. Pili-specific monoclonal antibodies reacted with either native pili or denatured pilin, and both demonstrated serotype specificity. Monoclonal antibodies to Bordetella pertussis OMPs were directed to either the virulent phase-regulated trypsin-sensitive, detergent-extractable OMPs 92 kDa, 32 kDa, and 30 kDa or the non-virulent phase-expressed, not-trypsin sensitive OMPs 38 kDa, 33kDa, and 18 kDa.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Poolman JT,Kuipers B,Vogel ML,Hamstra HJ,Nagel J

doi

10.1016/0882-4010(90)90024-k

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

377-82

issue

6

eissn

0882-4010

issn

1096-1208

journal_volume

8

pub_type

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