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Ligand specificity and affinity in the sulforhodamine B binding RNA aptamer.

Abstract：

:Binding affinity and selectivity are critical properties of aptamers that must be optimized for any application. The sulforhodamine B binding RNA aptamer (SRB-2) is a somewhat promiscuous aptamer that can bind ligands that vary markedly in shape, size and charge. Here we categorize potential ligands based on their binding mode and structural characteristics required for high affinity and selectivity. Several known and potential ligands of SRB-2 were screened for binding affinity using LSPR, ITC and NMR spectroscopy. The study shows that rhodamine B has the ideal structural and electrostatic properties for selective and high-affinity binding of the SRB-2 aptamer.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Piccolo KA,McNeil B,Crouse J,Lim SJ,Bickers SC,Hopkins WS,Dieckmann T

doi

10.1016/j.bbrc.2020.06.056

subject

Has Abstract

pub_date

2020-08-27 00:00:00

pages

666-671

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)31272-9

journal_volume

529

pub_type

杂志文章

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