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Electrophysiology of a chick neuronal nicotinic acetylcholine receptor expressed in Xenopus oocytes after cDNA injection.

Abstract：

:Brain nicotinic acetylcholine receptors (nAChRs) are made up of protein subunits that differ from those constituting muscle nAChRs. To characterize the physiological properties of one class of avian brain nicotinic receptor, we injected the nuclei of Xenopus oocytes with full-length cDNAs for the ligand binding (alpha 4) and structural (n alpha) subunits. Injected oocytes had large ACh-induced currents in the microampere range that were insensitive to alpha-bungarotoxin, as expected for neuronal nAChRs. We found that these brain nAChRs incorporate at least two alpha 4 subunits and that their functional properties differ from muscle nAChRs in at least two respects: the elementary conductance is considerably smaller (20 pS), and channels in outside out patches stop functioning within a few minutes.

journal_name

Neuron

journal_title

Neuron

authors

Ballivet M,Nef P,Couturier S,Rungger D,Bader CR,Bertrand D,Cooper E

doi

10.1016/0896-6273(88)90132-8

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

847-52

issue

9

eissn

0896-6273

issn

1097-4199

pii

0896-6273(88)90132-8

journal_volume

1

pub_type

杂志文章

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