Abstract:
:Presynaptic CaV2 channels are essential for Ca2+-triggered exocytosis. In addition, there are two competing models for their roles in synapse structure. First, Ca2+ channels or Ca2+ entry may control synapse assembly. Second, active zone proteins may scaffold CaV2s to presynaptic release sites, and synapse structure is CaV2 independent. Here, we ablated all three CaV2s using conditional knockout in cultured hippocampal neurons or at the calyx of Held, which abolished evoked exocytosis. Compellingly, synapse and active zone structure, vesicle docking, and transsynaptic nano-organization were unimpaired. Similarly, long-term blockade of action potentials and Ca2+ entry did not disrupt active zone assembly. Although CaV2 knockout impaired the localization of β subunits, α2δ-1 localized normally. Rescue with CaV2 restored exocytosis, and CaV2 active zone targeting depended on the intracellular C-terminus. We conclude that synapse assembly is independent of CaV2s or Ca2+ entry through them. Instead, active zone proteins recruit and anchor CaV2s via CaV2 C-termini.
journal_name
Neuronjournal_title
Neuronauthors
Held RG,Liu C,Ma K,Ramsey AM,Tarr TB,De Nola G,Wang SSH,Wang J,van den Maagdenberg AMJM,Schneider T,Sun J,Blanpied TA,Kaeser PSdoi
10.1016/j.neuron.2020.05.032subject
Has Abstractpub_date
2020-08-19 00:00:00pages
667-683.e9issue
4eissn
0896-6273issn
1097-4199pii
S0896-6273(20)30401-3journal_volume
107pub_type
杂志文章相关文献
NEURON文献大全abstract::The product of the Wnt-1 proto-oncogene is a secreted glycoprotein that is normally produced in regions of the embryonic neural tube. We show here that expression of mouse Wnt-1 cDNA in the rat PC12 pheochromocytoma cell line causes a dramatic conversion from a round to a flat cell morphology. In addition, PC12 cells ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90116-9
更新日期:1993-11-01 00:00:00
abstract::In this issue of Neuron, a study by Baumgartner et al. investigates the influence of oxytocin on trust behavior and its neural mechanisms. The authors report that, following breaches of trust, oxytocin facilitates prosocial behavior while modulating neural signals in the amygdala and caudate nucleus. The findings have...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2008.05.005
更新日期:2008-05-22 00:00:00
abstract::The N-methyl-D-aspartate (NMDA) subtype of glutamate receptor is important for synaptic plasticity and nervous system development and function. We have used genetic and electrophysiological methods to demonstrate that NMR-1, a Caenorhabditis elegans NMDA-type ionotropic glutamate receptor subunit, plays a role in the ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(01)00394-4
更新日期:2001-08-30 00:00:00
abstract::RNA transport is an important and fundamental event for local protein synthesis, especially in neurons. RNA is transported as large granules, but little is known about them. Here, we isolated a large RNase-sensitive granule (size: 1000S approximately) as a binding partner of conventional kinesin (KIF5). We identified ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2004.07.022
更新日期:2004-08-19 00:00:00
abstract::Autism spectrum disorder (ASD) is a heritable, common neurodevelopmental disorder with diverse genetic causes. Several studies have implicated protein synthesis as one among several of its potential convergent mechanisms. We originally identified Janus kinase and microtubule-interacting protein 1 (JAKMIP1) as differen...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.10.031
更新日期:2015-12-16 00:00:00
abstract::Human vision adapts to the contrast of patterns by changing its sensitivity, but the origins of this perceptual adaptation have been disputed. In this issue of Neuron, Solomon et al. show that contrast adaptation in the primate arises mostly in the retina for the magnocellular pathway and mostly in the cortex for the ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/s0896-6273(04)00187-4
更新日期:2004-04-08 00:00:00
abstract::Although prefrontal cortex has been implicated in the cognitive regulation of emotion, the cortical-subcortical interactions that mediate this ability remain poorly understood. To address this issue, we identified a right ventrolateral prefrontal region (vlPFC) whose activity correlated with reduced negative emotional...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.09.006
更新日期:2008-09-25 00:00:00
abstract::The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is involved in many cellular processes, including cell growth and differentiation, immune functions and cancer. It is activated by various cytokines, growth factors, and protein tyrosine kinases (PTKs) and regulates the transcriptio...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2011.11.024
更新日期:2012-01-26 00:00:00
abstract::Primary nociceptors relay painful touch information from the periphery to the spinal cord. Although it is established that signals generated by receptor tyrosine kinases TrkA and Ret coordinate the development of distinct nociceptive circuits, mechanisms modulating TrkA or Ret pathways in developing nociceptors are un...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.04.009
更新日期:2014-05-07 00:00:00
abstract::Cued spatial attention differentially modulates alpha power in attended relative to non-attended brain representations, termed the alpha asymmetry. Yet a causal role for alpha in attention is debated. In this issue of Neuron, Bagherzadeh et al., (2019) utilize neurofeedback to train alpha asymmetry and causally impact...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2019.12.033
更新日期:2020-02-05 00:00:00
abstract::The panoply of cognitive, affective, motivational, and social functions that underpin everyday human experience requires precisely choreographed patterns of interaction between networked brain regions. Perhaps not surprisingly, diverse forms of psychopathology are characterized by breakdowns in these interregional rel...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2012.06.002
更新日期:2012-06-21 00:00:00
abstract::It has been postulated that selective temporal coordination between neurons and development of functional neuronal assemblies are fundamental for brain function and behavior. Still, there is little evidence that functionally relevant coordination emerges preferentially in neuronal assemblies directly controlling behav...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.06.047
更新日期:2013-09-04 00:00:00
abstract::In this issue of Neuron, Wang et al. demonstrate that both cell-intrinsic and -extrinsic factors restrict the myelination of newly regenerated axons. Pharmalogical targeting of GPR17 signaling in oligodendrocyte precursor cells (OPCs) and microglial inhibition of oligodendrocyte maturation together promote robust myel...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2020.10.033
更新日期:2020-12-09 00:00:00
abstract::Asymmetric Ca(2+) elevations across the axonal growth cone mediate its turning responses to attractive and repulsive guidance cues. Here we show that clathrin-mediated endocytosis acts downstream of Ca(2+) signals as driving machinery for growth cone turning. In dorsal root ganglion neurons, the formation of clathrin-...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2010.04.007
更新日期:2010-05-13 00:00:00
abstract::The role of the target cell in neurotrophin-induced modifications of glutamatergic synaptic transmission was examined in cultured hippocampal neurons. Brain-derived neurotrophic factor (BDNF) induced rapid and persistent potentiation of evoked glutamate release when the postsynaptic neuron was glutamatergic, or excita...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80879-x
更新日期:2000-01-01 00:00:00
abstract::Telling time and anticipating when external events will happen is among the most important tasks the brain performs. Yet the neural mechanisms underlying timing remain elusive. One theory proposes that timing is a general and intrinsic computation of cortical circuits. We tested this hypothesis using electrical and op...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2016.05.042
更新日期:2016-07-20 00:00:00
abstract::Neural circuits consist of highly precise connections among specific types of neurons that serve a common functional goal. How neurons distinguish among different synaptic targets to form functionally precise circuits remains largely unknown. Here, we show that during development, the adhesion molecule cadherin-6 (Cdh...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2011.07.006
更新日期:2011-08-25 00:00:00
abstract::Oxytocin (OT) release by axonal terminals onto the central nucleus of the amygdala exerts anxiolysis. To investigate which subpopulation of OT neurons contributes to this effect, we developed a novel method: virus-delivered genetic activity-induced tagging of cell ensembles (vGATE). With the vGATE method, we identifie...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.04.029
更新日期:2019-07-03 00:00:00
abstract::A new fluorescence method using the dye FM1-43 was used to examine exocytotic release from hippocampal synaptosomes. Nitric oxide caused a marked transient stimulation of vesicle release. Several structurally unrelated nitric oxide donors, sodium nitroprusside, S-nitroso-N-acetylpenicillamine, 3-morpholino-sydnonimine...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90440-5
更新日期:1994-06-01 00:00:00
abstract::In vivo optogenetics provides unique, powerful capabilities in the dissection of neural circuits implicated in neuropsychiatric disorders. Conventional hardware for such studies, however, physically tethers the experimental animal to an external light source, limiting the range of possible experiments. Emerging wirele...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2016.12.031
更新日期:2017-02-08 00:00:00
abstract::Direct brain stimulation has diverse effects on network activity. In this issue of Neuron, Qiao et al. (2020) demonstrate a novel approach for using stimulation to characterize and modulate interactions between areas of the mood processing network. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2020.08.008
更新日期:2020-09-09 00:00:00
abstract::Virtually all stages of the visual system exhibit adaptation: neurons adjust their responses based on the recent stimulus history. While some of these adjustments occur at specific stages, others may be inherited from earlier stages. How do adaptation effects cascade along the visual system? We measured spatially sele...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.11.025
更新日期:2014-02-05 00:00:00
abstract::Certain neurons in the mammalian brain have long been known to be joined by gap junctions, which are the most common type of electrical synapse. More recently, cloning of neuron-specific connexins, increased capability of visualizing cells within brain tissue, labeling of cell types by transgenic methods, and generati...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/s0896-6273(04)00043-1
更新日期:2004-02-19 00:00:00
abstract::Quantitative differences in cadherin activity have been proposed to play important roles in patterning connections between pre- and postsynaptic neurons. However, no examples of such a function have yet been described, and the mechanisms that would allow such differences to direct growth cones to specific synaptic tar...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.01.007
更新日期:2008-04-10 00:00:00
abstract::GABA(B) (gamma-aminobutyric acid type B) receptors are important for keeping neuronal excitability under control. Cloned GABA(B) receptors do not show the expected pharmacological diversity of native receptors and it is unknown whether they contribute to pre- as well as postsynaptic functions. Here, we demonstrate tha...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(01)00345-2
更新日期:2001-07-19 00:00:00
abstract::Activity is known to be important for the refinement of neural connections in the developing brain. In this issue of Neuron, Hanson and Landmesser provide evidence that GABA-dependent spontaneous bursting of motor neurons in the embryonic spinal cord is required for the correct execution of an early axon pathfinding d...
journal_title:Neuron
pub_type: 评论,杂志文章,评审
doi:10.1016/j.neuron.2004.08.027
更新日期:2004-09-02 00:00:00
abstract::Regulation of synaptic strength is essential for neuronal information processing, but the molecular mechanisms that control changes in neuroexocytosis are only partially known. Here we show that the putative G protein-coupled receptor Methuselah (Mth) is required in the presynaptic motor neuron to acutely upregulate n...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)00932-7
更新日期:2002-09-26 00:00:00
abstract::In this issue of Neuron, Kraus et al. (2013) show that a population of "time cells" in the hippocampus responds to the passage of time rather than simply reflecting path integration. This study advances our understanding of how time is represented in the hippocampus. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2013.05.039
更新日期:2013-06-19 00:00:00
abstract::Olfactory receptor neurons (ORNs) do not express the typical neuronal intermediate filament proteins (IFPs), the neurofilament triplet proteins. Immunocytochemical evidence shows that ORNs coexpress vimentin and peripherin but distribute them differently. Specifically, ORNs contain vimentin in dendrites, cell bodies, ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90300-o
更新日期:1991-09-01 00:00:00
abstract::Cortical GABAergic interneurons have essential roles for information processing and their dysfunction is implicated in neuropsychiatric disorders. Transcriptional codes are elucidating mechanisms of interneuron specification in the MGE (a subcortical progenitor zone), which regulate their migration, integration, and f...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.02.030
更新日期:2014-04-16 00:00:00