Abstract:
:Hepatitis C virus (HCV) core protein forms the nucleocapsid of the HCV particle. Although many functions of core protein have been reported, how the HCV particle is assembled is not well understood. Here we show that the nucleocapsid-like particle of HCV is composed of a disulfide-bonded core protein complex (dbc-complex). We also found that the disulfide-bonded dimer of the core protein (dbd-core) is formed at the endoplasmic reticulum (ER), where the core protein is initially produced and processed. Mutational analysis revealed that the cysteine residue at amino acid position 128 (Cys128) of the core protein, a highly conserved residue among almost all reported isolates, is responsible for dbd-core formation and virus-like particle production but has no effect on the replication of the HCV RNA genome or the several known functions of the core protein, including RNA binding ability and localization to the lipid droplet. The Cys128 mutant core protein showed a dominant negative effect in terms of HCV-like particle production. These results suggest that this disulfide bond is critical for the HCV virion. We also obtained the results that the dbc-complex in the nucleocapsid-like structure was sensitive to proteinase K but not trypsin digestion, suggesting that the capsid is built up of a tightly packed structure of the core protein, with its amino (N)-terminal arginine-rich region being concealed inside.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kushima Y,Wakita T,Hijikata Mdoi
10.1128/JVI.00402-10subject
Has Abstractpub_date
2010-09-01 00:00:00pages
9118-27issue
18eissn
0022-538Xissn
1098-5514pii
JVI.00402-10journal_volume
84pub_type
杂志文章abstract::Immediate-early viral gene products of human cytomegalovirus (HCMV) are derived from several genomic loci and largely serve to establish a cellular environment conducive to viral replication. We have further examined an unusual immediate-early transcript known as the 5-kb RNA, concluding that it is a stable intron enc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13182-13189.2004
更新日期:2004-12-01 00:00:00
abstract::Infection of Escherichia coli with the satellite virus P4 without its helper bacteriophage P2 leads either to the immune integrated state or to the nonimmune multicopy plasmid condition. We analyzed the transcription pattern of the phage plasmid P4 early and late after infection and during the stable plasmid or lysoge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1697-1704.1988
更新日期:1988-05-01 00:00:00
abstract::In an effort to develop a useful AIDS vaccine or vaccine component, we have generated a combinatorial library of chimeric viruses in which the sequence IGPGRAFYTTKN from the V3 loop of the MN strain of human immunodeficiency virus type 1 (HIV-1) is displayed in many conformations on the surface of human rhinovirus 14 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.651-659.1998
更新日期:1998-01-01 00:00:00
abstract::We describe respiratory syncytial virus (RSV)-specific cytotoxic T-cell (CTL) lines and clones developed from the spleens of C57BL/6 and BALB/c mice. Line 7 and clones derived from it were H-2Kb restricted, whereas line 12 had both Kb and Db components. Both lines, and all the clones except one, could lyse targets inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4494-4497.1991
更新日期:1991-08-01 00:00:00
abstract::Rift Valley fever virus (RVFV), a phlebovirus of the Bunyaviridae family, is an arthropod-borne virus which emerges periodically throughout Africa, emphasizing that it poses a major threat for animal and human populations. To assess the genetic variability of RVFV, several isolates from diverse localities of Africa we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8196-8200.1999
更新日期:1999-10-01 00:00:00
abstract::Live attenuated human immunodeficiency virus type 1 (HIV-1) vaccines are considered unsafe because faster-replicating pathogenic virus variants may evolve after vaccination. We previously presented a conditional-live HIV-1 variant of which replication can be switched off as an alternative vaccination strategy. To impr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3855-3858.2005
更新日期:2005-03-01 00:00:00
abstract::Cyclosporine (CsA) and its derivatives potently suppress hepatitis C virus (HCV) replication. Recently, CsA-resistant HCV replicons have been identified in vitro. We examined the dependence of the wild-type and CsA-resistant replicons on various cyclophilins for replication. A strong correlation between CsA resistance...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02614-07
更新日期:2008-06-01 00:00:00
abstract::The viral dynamics in human immunodeficiency virus type 1 (HIV-1) infection have been studied extensively using mathematical modeling, but data from other primate lentivirus systems are scarce. This study was initiated to increase the understanding of the differences and similarities between the different primate lent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00394-06
更新日期:2006-10-01 00:00:00
abstract::Regulation of immediate-early gene expression in human cytomegalovirus is subject to complex controls. The major immediate-early (mIE) gene is regulated by both positive and negative regulatory signals, including autoregulation mediated by a cis-repressive sequence. A second immediate-early gene, the US3 gene, is tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5362-5367.1995
更新日期:1995-09-01 00:00:00
abstract::Rubella virus contains three structural proteins, capsid, E2, and E1. E2 and E1 are type I membrane glycoproteins that form a heterodimer in the endoplasmic reticulum (ER) before they are transported to and retained in the Golgi complex, where virus assembly occurs. The bulk of unassembled E2 and E1 subunits are not t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7670-7680.1997
更新日期:1997-10-01 00:00:00
abstract::The varicella-zoster virus major transactivator, IE62, contains a potent N-terminal acidic transcriptional activation domain (TAD). Our experiments revealed that the minimal IE62 TAD encompasses amino acids (aa) 19 to 67. We showed that the minimal TAD interacts with the human Mediator complex. Site-specific mutations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00054-09
更新日期:2009-06-01 00:00:00
abstract::The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.677-689.1974
更新日期:1974-03-01 00:00:00
abstract::We have been studying defective interfering (DI) genomes of the RNA enveloped virus Sindbis virus. Deletion mapping of a DI cDNA demonstrated that only sequences at the 3' and 5' termini of the genome are required for the DI RNA to be biologically active. We constructed a series of cDNAs that transcribe DI RNAs differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.47-53.1988
更新日期:1988-01-01 00:00:00
abstract::Pretreatment with two biological response modifiers (BRM), OK-432 and PS-K, protected mice from lethal infection by murine cytomegalovirus (MCMV). This was evidenced by an increase in 50% lethal doses and a decrease in titers of infectious viruses replicated in the liver and spleen. Spleen cells from the BRM-treated m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.117-122.1984
更新日期:1984-07-01 00:00:00
abstract::Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) and CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BI and SR-BII, which differ in their C termini. SR-BI...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02356-06
更新日期:2007-04-01 00:00:00
abstract::Persistent infection with oncogenic human papillomaviruses (HPVs) causes cervical cancer, accompanied by the accumulation of somatic mutations into the host genome. There are concomitant genetic changes in the HPV genome during viral infection; however, their relevance to cervical carcinogenesis is poorly understood. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00017-18
更新日期:2018-05-29 00:00:00
abstract::The importance of neutralizing antibodies (NAbs) in protection against hepatitis C virus (HCV) remains controversial. We infused a chimpanzee with H06 immunoglobulin from a genotype 1a HCV-infected patient and challenged with genotype strains efficiently neutralized by H06 in vitro. Genotype 1a NAbs afforded no protec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01194-15
更新日期:2015-09-01 00:00:00
abstract::The induction of alpha/beta interferon (IFN-alpha/beta) genes constitutes one of the first responses of the cell to virus infection. The IFN-beta gene is constitutively repressed in uninfected cells and is transiently activated after virus infection. In this work we demonstrate that histone deacetylation regulates the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3444-3452.2001
更新日期:2001-04-01 00:00:00
abstract::A human cytomegalovirus (CMV) UL54 pol exonuclease domain II mutation, D413A, found in a clinical specimen, conferred ganciclovir (GCV) and cidofovir resistance but not foscarnet resistance when incorporated into laboratory strain T2294. After several passages without drug, mutation was observed in five of eight plaqu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-07
更新日期:2008-01-01 00:00:00
abstract::Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with the major viral proteins, p40 and p23. Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments and synthetic peptides were analyzed to characterize the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8133-8137.1996
更新日期:1996-11-01 00:00:00
abstract::Herein we demonstrate that chronic simian immunodeficiency virus (SIV) infection induces significant upregulation of the gut-homing marker alpha4beta7 on macaque NK cells, coupled with downregulation of the lymph node-trafficking marker, CCR7. Interestingly, in naïve animals, alpha4beta7 expression was associated with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01126-10
更新日期:2010-09-01 00:00:00
abstract::Small amounts of infectious simian virus 40 (SV40) were recovered from parental cultures of SV40-transformed human embryonic lung (WI38 Va13A) cells, from 12 primary clones, from 17 secondary clones, and from 18 tertiary clones. The cloning experiments demonstrated that the capacity for spontaneous virus production is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.4.430-436.1971
更新日期:1971-10-01 00:00:00
abstract::Epstein-Barr virus (EBV) efficiently drives proliferation of human primary B cells in vitro, a process relevant for human diseases such as infectious mononucleosis and posttransplant lymphoproliferative disease. Human B-cell proliferation is also driven by ligands of Toll-like receptors (TLRs), notably viral or bacter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01400-09
更新日期:2010-04-01 00:00:00
abstract::A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.924-933.1974
更新日期:1974-10-01 00:00:00
abstract::Productive infection by the murine autonomous parvovirus minute virus of mice (MVM) depends on a dividing cell population and its differentiation state. We have extended the in vivo analysis of the MVM host cell type range into the developing embryo by in utero inoculation followed by further gestation. The fibrotropi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9474-9486.2004
更新日期:2004-09-01 00:00:00
abstract::Specific cleavage of BK virus (MM) DNA with restriction endonuclease MboI gives rise to 10 fragments. Two techniques were used to determine the location of these fragments on the viral genome with respect to the three known sites for HindIII cleavage. In the first method, reciprocal digestion, individual MboI fragment...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.700-712.1978
更新日期:1978-09-01 00:00:00
abstract::Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7006-7014.1995
更新日期:1995-11-01 00:00:00
abstract::Nevirapine forms the mainstay of our efforts to curtail the pediatric AIDS epidemic through prevention of mother-to-child transmission of HIV-1. A key limitation, however, is the rapid selection of HIV-1 strains resistant to nevirapine following the administration of a single dose. This rapid selection of resistance s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01010-10
更新日期:2010-10-01 00:00:00
abstract::Three serial passage series of simian virus 40 (SV40) in CV-1 cells were initiated by infection directly from the same wild-type plaque isolate, three series were initiated by infection with another plaque isolate, and two series were initiated with each of two other plaque isolates. Aberrant SV40 genomes were not det...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.730-733.1982
更新日期:1982-05-01 00:00:00
abstract::Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02941-13
更新日期:2014-02-01 00:00:00