Abstract：
:It is well established that high-LET radiations efficiently induce chromosome aberrations. However, data on the effect of protons on telomere maintenance, as involved in genomic stability, are scarce and contradictory. Here we demonstrate that high-LET protons induce telomere lengthening in human primary fibroblasts and that this elongation does not involve the telomerase enzyme, supporting the hypothesis that high-LET radiations are able to activate a telomerase-independent mechanism. In tumor cells that lack telomerase, one or more non-telomerase mechanisms for telomere maintenance are present, which are termed alternative lengthening of telomeres (ALT). Since ALT cells are characterized by recombinational events at telomeres, known as telomeric-sister chromatid exchanges (T-SCE), and colocalization of telomeres and premyelocytic leukemia protein (PML), we analyzed both T-SCE and PML. Our results show that high-LET protons induce a 2.5-fold increase of T-SCE and a colocalization of PML protein and telomeric DNA. Furthermore, our data show that the ALT pathway can be activated in human primary cells after induction of severe DNA damage. Thus, since telomeres are known to be involved in chromosome maintenance, the present work may contribute in the elucidation of the mechanism by which ionizing radiation induces genomic instability.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Berardinelli F,Antoccia A,Cherubini R,De Nadal V,Gerardi S,Cirrone GA,Tanzarella C,Sgura Adoi
10.1667/RR2127.1subject
Has Abstractpub_date
2010-11-01 00:00:00pages
539-49issue
5eissn
0033-7587issn
1938-5404journal_volume
174pub_type
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journal_title：Radiation research
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doi：
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doi：
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doi：
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doi：
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doi：
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doi：
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doi：
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