当前位置： SCI文献检索 > RADIATION RESEARCH期刊下所有文献 > MicroRNA Analysis of ATM-Deficient Cells Indicate PTEN and CCDN1 as Potential Biomarkers of Radiation Response.

MicroRNA Analysis of ATM-Deficient Cells Indicate PTEN and CCDN1 as Potential Biomarkers of Radiation Response.

Abstract：

:Genetic and epigenetic profile changes associated with individual radiation sensitivity are well documented and have led to enhanced understanding of the mechanisms of the radiation-induced DNA damage response. However, the search continues to identify reliable biomarkers of individual radiation sensitivity. Herein, we report on a multi-biomarker approach using traditional cytogenetic biomarkers, DNA damage biomarkers and transcriptional microRNA (miR) biomarkers coupled with their potential gene targets to identify radiosensitivity in ataxia-telangiectasia mutated (ATM)-deficient lymphoblastoid cell lines (LCL); ATM-proficient cell lines were used as controls. Cells were 0.05 and 0.5 Gy irradiated, using a linear accelerator, with sham-irradiated cells as controls. At 1 h postirradiation, cells were fixed for γ-H2AX analysis as a measurement of DNA damage, and cytogenetic analysis using the G2 chromosomal sensitivity assay, G-banding and FISH techniques. RNA was also isolated for genetic profiling by microRNA (miR) and RT-PCR analysis. A panel of 752 miR were analyzed, and potential target genes, phosphatase and tensin homolog (PTEN) and cyclin D1 (CCND1), were measured. The cytogenetic assays revealed that although the control cell line had functional cell cycle checkpoints, the radiosensitivity of the control and AT cell lines were similar. Analysis of DNA damage in all cell lines, including an additional control cell line, showed elevated γ-H2AX levels for only one AT cell line. Of the 752 miR analyzed, eight miR were upregulated, and six miR were downregulated in the AT cells compared to the control. Upregulated miR-152-3p, miR-24-5p and miR-92-15p and all downregulated miR were indicated as modulators of PTEN and CCDN1. Further measurement of both genes validated their potential role as radiation-response biomarkers. The multi-biomarker approach not only revealed potential candidates for radiation response, but provided additional mechanistic insights into the response in AT-deficient cells.

journal_name

Radiat Res

journal_title

Radiation research

authors

Bryant J,White L,Coen N,Shields L,McClean B,Meade AD,Lyng FM,Howe O

doi

10.1667/RR15462.1

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

520-530

issue

6

eissn

0033-7587

issn

1938-5404

pii

436355

journal_volume

193

pub_type

杂志文章

相关文献

RADIATION RESEARCH文献大全
  • Performance of silicon microdosimetry detectors in boron neutron capture therapy.

    abstract：:Reverse-biased silicon p-n junction arrays using Silicon-On-Insulator technology have been proposed as microdosimeters. The performance of such detectors in boron neutron capture therapy (BNCT) is discussed. This work provides the first reported measurements using boron-coated silicon diode arrays as microdosimeters i...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Bradley PD,Rosenfeld AB,Allen B,Coderre J,Capala J

    更新日期：1999-03-01 00:00:00

  • A comparative study between individuals receiving thymic irradiation in infancy and their nontreated siblings: clinical and laboratory thyroid abnormalities.

    abstract：:While impairment of thyroid function has been demonstrated for high-dose external radiation (e.g., for Hodgkin's disease), the long-term functional effects of low-dose external radiation have not been fully explored. One hundred fifty-three subjects with a past history of thymic irradiation during infancy were stratif...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Hildreth NG,Schneider AB,Cave WT Jr

    更新日期：1987-06-01 00:00:00

  • Low-dose binary behavior of bystander cell killing after microbeam irradiation of a single cell with focused c(k) x rays.

    abstract：:Although conclusive evidence has been obtained for the presence of radiation-induced bystander effects, the mechanisms that trigger and regulate these processes are still largely unknown. The bystander effect may play a critical role in determining the biological effectiveness of low-dose exposures, but questions on h...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/rr3319

    authors： Schettino G,Folkard M,Michael BD,Prise KM

    更新日期：2005-03-01 00:00:00

  • Molecular analysis of base damage clustering associated with a site-specific radiation-induced DNA double-strand break.

    abstract：:Base damage flanking a radiation-induced DNA double-strand break (DSB) may contribute to DSB complexity and affect break repair. However, to date, an isolated radiation-induced DSB has not been assessed for such structures at the molecular level. In this study, an authentic site-specific radiation-induced DSB was prod...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR0628.1

    authors： Datta K,Jaruga P,Dizdaroglu M,Neumann RD,Winters TA

    更新日期：2006-11-01 00:00:00

  • Hyperthermic survival of Chinese hamster ovary cells as a function of cellular population density at the time of plating.

    abstract：:The survival of synchronous G1 or asynchronous Chinese hamster ovary cells in vitro to heat treatment may depend on the cellular population density at the time of heating and/or as the cells are cultured after heating. The addition of lethally irradiated feeder cells may increase survival at 10(-3) by as much as 10- t...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Highfield DP,Holahan EV,Holahan PK,Dewey WC

    更新日期：1984-01-01 00:00:00

  • X-ray- and ultraviolet-radiation-induced mutations in Muta mouse.

    abstract：:To evaluate the usefulness of the transgenic Muta mouse for investigating radiation-induced mutations in vivo, we have examined the effects of whole-body X irradiation and compared them to the effects of ultraviolet light. The spontaneous mutation frequencies in young adults were about 7 x 10(-5) in the spleen, liver ...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Ono T,Ikehata H,Hosoi Y,Shung BS,Kurishita A,Wang X,Yamamoto K,Suzuki T,Sofuni T

    更新日期：1997-08-01 00:00:00

  • Acute radiation-induced vomiting in area postrema-ablated cats.

    abstract：:A dose-response relationship was established in normal unanesthetized cats for emetic incidence, latency to onset of vomiting, and duration of emetic activity over a period of 24 h after whole-body exposure to 60Co radiation at selected doses between 7.5 and 90 Gy. Each episode of vomiting, i.e., retching and expulsio...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Borison HL,McCarthy LE,Douple EB,Johnson J,Borison R

    更新日期：1987-03-01 00:00:00

  • Prevalence of hyperthyroidism after exposure during childhood or adolescence to radioiodines from the chornobyl nuclear accident: dose-response results from the Ukrainian-American Cohort Study.

    abstract：:Relatively few data are available on the prevalence of hyperthyroidism (TSH concentrations of <0.3 mIU/liter, with normal or elevated concentrations of free T4) in individuals exposed to radioiodines at low levels. The accident at the Chornobyl (Chernobyl) nuclear plant in Ukraine on April 26, 1986 exposed large numbe...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR2003.1

    authors： Hatch M,Furukawa K,Brenner A,Olinjyk V,Ron E,Zablotska L,Terekhova G,McConnell R,Markov V,Shpak V,Ostroumova E,Bouville A,Tronko M

    更新日期：2010-12-01 00:00:00

  • Challenges and Benefits of Repurposing Products for Use during a Radiation Public Health Emergency: Lessons Learned from Biological Threats and other Disease Treatments.

    abstract：:The risk of a radiological or nuclear public health emergency is a major growing concern of the U.S. government. To address a potential incident and ensure that the government is prepared to respond to any subsequent civilian or military casualties, the U.S. Department of Health and Human Services and the Department o...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR15137.1

    authors： DiCarlo AL,Cassatt DR,Dowling WE,Esker JL,Hewitt JA,Selivanova O,Williams MS,Price PW

    更新日期：2018-12-01 00:00:00

  • Investigation of neutron-induced damage in DNA by atomic force microscopy: experimental evidence of clustered DNA lesions.

    abstract：:Using atomic force microscopy (AFM), we have investigated neutron-induced DNA double-strand breaks in plasmids in aqueous solution. AFM permits direct measurement of individual DNA molecules with an accuracy of a few nanometers. Furthermore, the analysis of the DNA fragment size distribution is non-parametric, whereas...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Pang D,Berman BL,Chasovskikh S,Rodgers JE,Dritschilo A

    更新日期：1998-12-01 00:00:00

  • Effects of partial hepatectomy on the growth characteristics and hypoxic fractions of xenografted DLD-2 human colon cancers.

    abstract：:A recent publication by Leith et al. (Cancer Res. 51, 4111-4113, 1991) showed that administration of epidermal growth factor (EGF) (0.25 mg/kg; q.i.d. x 7) to mice bearing xenografted A431 human epidermoid cancers produced increased tumor growth and a reduction of the percentage of hypoxic cells within neoplasms. In c...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Leith JT,Padfield G,Michelson S

    更新日期：1992-11-01 00:00:00

  • Development-dependent expression of DNA repair genes and epigenetic regulators in Arabidopsis plants exposed to ionizing radiation.

    abstract：:Both plant senescence and plant response to ionizing radiation involve changes in gene expression and epigenetic profiles, that rely on the formation of reactive oxygen species. However, how the developmental stage of a plant affects its response to ionizing radiation has not been extensively studied. In this study, o...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR13840.1

    authors： Sidler C,Li D,Kovalchuk O,Kovalchuk I

    更新日期：2015-02-01 00:00:00

  • Photodynamic properties of meta-tetra(hydroxyphenyl)chlorin in human tumor cells.

    abstract：:The photodynamic properties of a second-generation photodynamic sensitizer, meta-tetra(hydroxyphenyl)chlorin (mTHPC) were studied by dye-sensitized photoinactivation (650 nm) of HT29 human adenocarcinoma cells in culture. The photocytotoxicity of mTHPC in vitro depended on the presence of molecular oxygen. A strong in...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Melnikova VO,Bezdetnaya LN,Potapenko AY,Guillemin F

    更新日期：1999-10-01 00:00:00

  • Cerebrovascular response after interstitial irradiation.

    abstract：:To characterize the role of the cerebrovascular response in the development of brain injury after focal irradiation, 125I sources were implanted in frontal white matter of the brain of normal dogs; dose was 20 Gy, 7.5 mm from the source. Cerebral blood flow, vascular volume and mean transit time of blood were quantifi...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Gobbel GT,Seilhan TM,Fike JR

    更新日期：1992-05-01 00:00:00

  • Antifibrotic Agent Pirfenidone Protects against Development of Radiation-Induced Pulmonary Fibrosis in a Murine Model.

    abstract：:Radiation-induced complications of the respiratory system are a common side effect of thoracic radiotherapy with no viable treatment option. Here, we investigated the potential therapeutic effect of the orphan drug pirfenidone for treating radiation-induced pulmonary fibrosis. C57BL/6 mice received a single fraction o...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR15017.1

    authors： Qin W,Liu B,Yi M,Li L,Tang Y,Wu B,Yuan X

    更新日期：2018-10-01 00:00:00

  • Simulation of exposure and SAR estimation for adult and child heads exposed to radiofrequency energy from portable communication devices.

    abstract：:The level and distribution of radiofrequency energy absorbed in a child's head during the use of a mobile phone compared to those in an adult head has been a controversial issue in recent years. It has been suggested that existing methods that are used to determine specific absorption rate (SAR) and assess compliance ...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/rr3353

    authors： Bit-Babik G,Guy AW,Chou CK,Faraone A,Kanda M,Gessner A,Wang J,Fujiwara O

    更新日期：2005-05-01 00:00:00

  • Radiation damage to DNA base pairs. II. Paramagnetic resonance studies of 1-methyluracil x 9-ethyladenine complex crystals X-irradiated at 10 K.

    abstract：:Single crystals of the co-crystalline complex of 1-methyluracil and 9-ethyladenine were X-irradiated and studied using EPR, ENDOR and FSE spectroscopic techniques at 10 K. All together seven radicals were identified, and experimental evidence for at least one more species, as well as for a very low population of radic...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Sagstuen E,Hole EO,Nelson WH,Close DM

    更新日期：1998-02-01 00:00:00

  • The role of the tubulointerstitium in radiation-induced renal fibrosis.

    abstract：:The functional and morphological response of the remaining hypertrophied kidney in unilaterally nephrectomized rats to single doses of 0-20 Gy X rays was investigated. Functional and histological end points were assessed serially 4-24 weeks postirradiation. Renal irradiation led to time- and dose-dependent reductions ...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/0033-7587(2001)155[0481:trotti]2.0.co;2

    authors： Robbins ME,O'Malley Y,Zhao W,Davis CS,Bonsib SM

    更新日期：2001-03-01 00:00:00

  • Small doses of high-linear energy transfer radiation increase the radioresistance of Chinese hamster V79 cells to subsequent X irradiation.

    abstract：:Chinese hamster V79 cells show a complex X-ray survival response which is characterized by hypersensitivity followed by increased resistance as the dose increases to 1 Gy. This hypersensitivity can be eliminated by pretreating cells with X rays or hydrogen peroxide. Accordingly, the protective effect that results from...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Marples B,Skov KA

    更新日期：1996-10-01 00:00:00

  • Effects of X-irradiation on the immune response of guinea pigs to Q fever vaccine.

    abstract：:The immune response of guinea pigs to Q fever vaccine following 75 to 250 R (60 to 180 rads) of acute whole-body irradiations was investigated. Complement-fixing (CF) antibody titers and protection against febrile response to challenge with virulent Coxiella burnetii were studied. Exposures ranging from 75 to 250 R, 2...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Reynolds SL,Whitford HW,Blemly NR,Staab EV,Craig CP

    更新日期：1968-10-01 00:00:00

  • An analysis of interplanetary space radiation exposure for various solar cycles.

    abstract：:The radiation dose received by crew members in interplanetary space is influenced by the stage of the solar cycle. Using the recently developed models of the galactic cosmic radiation (GCR) environment and the energy-dependent radiation transport code, we have calculated the dose at 0 and 5 cm water depth; using a com...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Badhwar GD,Cucinotta FA,O'Neill PM

    更新日期：1994-05-01 00:00:00

  • Monohydroxylated fatty acid content in peripheral blood mononuclear cells and immune status of people at long times after the Chernobyl accident.

    abstract：:The monohydroxylated fatty acid content of peripheral blood mononuclear cells from 23 cleanup workers and 16 unexposed individuals was studied in relation to their immune status after the Chernobyl accident. Men with absorbed doses below 0.32 Gy showed higher levels of free and esterified 12-hydroxyeicosatetraenoic ac...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/0033-7587(2001)156[0476:mfacip]2.0.co;2

    authors： Chumak A,Thevenon C,Gulaya N,Guichardant M,Margitich V,Bazyka D,Kovalenko A,Lagarde M,Prigent AF

    更新日期：2001-11-01 00:00:00

  • A lower dose threshold for the in vivo protective adaptive response to radiation. Tumorigenesis in chronically exposed normal and Trp53 heterozygous C57BL/6 mice.

    abstract：:Low doses of ionizing radiation to cells and animals may induce adaptive responses that reduce the risk of cancer. However, there are upper dose thresholds above which these protective adaptive responses do not occur. We have now tested the hypothesis that there are similar lower dose thresholds that must be exceeded ...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR1414.1

    authors： Mitchel RE,Burchart P,Wyatt H

    更新日期：2008-12-01 00:00:00

  • Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN Workshop.

    abstract：:In recent years, there has been increasing concern over the possibility of a radiological or nuclear incident occurring somewhere in the world. Intelligence agencies frequently report that terrorist groups and rogue nations are seeking to obtain radiological or nuclear weapons of mass destruction. In addition, there e...

    journal_title：Radiation research

    pub_type：

    doi：10.1667/RR14810.1

    authors： DiCarlo AL,Tamarat R,Rios CI,Benderitter M,Czarniecki CW,Allio TC,Macchiarini F,Maidment BW,Jourdain JR

    更新日期：2017-08-01 00:00:00

  • Imaging radiation-induced normal tissue injury.

    abstract：:Technological developments in radiation therapy and other cancer therapies have led to a progressive increase in five-year survival rates over the last few decades. Although acute effects have been largely minimized by both technical advances and medical interventions, late effects remain a concern. Indeed, the need t...

    journal_title：Radiation research

    pub_type： 杂志文章,评审

    doi：10.1667/rr2530.1

    authors： Robbins ME,Brunso-Bechtold JK,Peiffer AM,Tsien CI,Bailey JE,Marks LB

    更新日期：2012-04-01 00:00:00

  • Cerebrovascular Remodeling and Neuroinflammation is a Late Effect of Radiation-Induced Brain Injury in Non-Human Primates.

    abstract：:Fractionated whole-brain irradiation (fWBI) is a mainstay of treatment for patients with intracranial neoplasia; however late-delayed radiation-induced normal tissue injury remains a major adverse consequence of treatment, with deleterious effects on quality of life for affected patients. We hypothesize that cerebrova...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/RR14616.1

    authors： Andrews RN,Metheny-Barlow LJ,Peiffer AM,Hanbury DB,Tooze JA,Bourland JD,Hampson RE,Deadwyler SA,Cline JM

    更新日期：2017-05-01 00:00:00

  • Tumor induction and life shortening in BC3F1 female mice at low doses of fast neutrons and X rays.

    abstract：:Extension of previous investigations at this laboratory regarding life shortening and tumor induction in the mouse has provided more complete dose-response information in the low dose region of X rays and neutrons. A complete observation of survival and late pathology has been carried out on over 2000 BC3F1 female mic...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Covelli V,Coppola M,Di Majo V,Rebessi S,Bassani B

    更新日期：1988-02-01 00:00:00

  • Immunofluorescence detection of clustered gamma-H2AX foci induced by HZE-particle radiation.

    abstract：:We studied the spatial and temporal distributions of foci of the phosphorylated form of the histone protein H2AX (gamma-H2AX), which is known to be activated by double-strand breaks after irradiation of human fibroblast cells with high-energy silicon (54 keV/microm) and iron (176 keV/microm) ions. Here we present data...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/rr3431.1

    authors： Desai N,Davis E,O'Neill P,Durante M,Cucinotta FA,Wu H

    更新日期：2005-10-01 00:00:00

  • Effect of keratinocyte growth factor on radiation survival and colony size of human epidermal keratinocytes in vitro.

    abstract：:Keratinocyte growth factor (FGF7, also known as KGF) ameliorates the radiation response of mouse oral mucosa and other epithelial tissues. However, the precise mechanisms remain unclear. The aim of the present study was to investigate the effect of FGF7 on the survival and colony size of normal human epidermal keratin...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：10.1667/0033-7587(2001)156[0761:eokgfo]2.0.co;2

    authors： Slonina D,Hoinkis C,Dörr W

    更新日期：2001-12-01 00:00:00

  • Nonlinear survival curves for cells of solid tumors after large doses of fast neutrons and gamma rays.

    abstract：:We have previously proposed that survival curves for cells of murine NFSa fibrosarcomas after exposure to fast neutrons might demonstrate curvature when the neutron doses reach a level high enough to cure the fibrosarcomas. We report here that this is the case. Murine NFSa fibrosarcomas growing in the hind legs of syn...

    journal_title：Radiation research

    pub_type： 杂志文章

    doi：

    authors： Ando K,Koike S,Sato S

    更新日期：1992-08-01 00:00:00