A dual switch in phloem unloading during ovule development in Arabidopsis.

abstract：:Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...

journal_title：Protoplasma

authors： Gügel IL,Soll J

更新日期：2017-09-01 00:00:00

abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...

journal_title：Protoplasma

authors： Talbot MJ,Offler CE,McCurdy DW

更新日期：2002-05-01 00:00:00

abstract：:Turions, which are modified shoot apices, are vegetative, dormant overwintering organs produced by perennial aquatic plants. In this study, the turion cytochemistry and ultrastructure of Aldrovanda vesiculosa, Utricularia vulgaris and U. stygia were compared with particular emphasis placed on storage substances. These...

journal_title：Protoplasma

authors： Płachno BJ,Adamec L,Kozieradzka-Kiszkurno M,Swiątek P,Kamińska I

更新日期：2014-11-01 00:00:00

abstract：:The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...

journal_title：Protoplasma

authors： Lütz C

更新日期：2010-08-01 00:00:00

abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

journal_title：Protoplasma

authors： Brown RC,Lemmon BE

更新日期：2009-10-01 00:00:00

abstract：:Fragile sites (FSs) in plants have been described for species like Lolium and other grasses. Whereas in humans FSs were shown to be involved in genome instabilities; the consequences of FSs expression in plants are not known yet. To evaluate whether FSs cause karyotype instabilities, we assessed the frequency of micro...

journal_title：Protoplasma

authors： Rocha LC,Jankowska M,Fuchs J,Mittelmann A,Techio VH,Houben A

更新日期：2017-01-01 00:00:00

abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...

journal_title：Protoplasma

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Tuleja M,Ślesak H,Kapusta P,Marcińska I,Bohdanowicz J

更新日期：2013-02-01 00:00:00

abstract：:Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...

journal_title：Protoplasma

authors： Motes CM,Pechter P,Yoo CM,Wang YS,Chapman KD,Blancaflor EB

更新日期：2005-12-01 00:00:00

abstract：:Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...

journal_title：Protoplasma

authors： Duarte VP,Pereira MP,Corrêa FF,de Castro EM,Pereira FJ

更新日期：2021-01-06 00:00:00

abstract：:In order to gain more insight into the involvement of mitochondrial complex III in the Cd-induced stress, we studied the effect of complex III inhibitors, antimycin A (AA), and myxothiazol (MYXO), on the Cd-induced ROS and NO generation in the barley root tip. Short-term exposure of barley roots to either MYXO or AA p...

journal_title：Protoplasma

authors： Zelinová V,Demecsová L,Tamás L

更新日期：2019-09-01 00:00:00

abstract：:Temperatures above the optimum are sensed as heat stress (HS) by all living organisms and represent one of the major environmental challenges for plants. Plants can cope with HS by activating specific defense mechanisms to minimize damage and ensure cellular functionality. One of the most common effects of HS is the o...

journal_title：Protoplasma

authors： Malerba M,Cerana R

更新日期：2018-07-01 00:00:00

abstract：:Indeterminate growth and the production of new organs in plants require a constant supply of new cells. The majority of these cells are produced in mitotic regions called meristems. For primary or tip growth of the roots and shoots, the meristems are located in the apices. These apical meristems have been shown to fun...

journal_title：Protoplasma

authors： Matte Risopatron JP,Sun Y,Jones BJ

更新日期：2010-12-01 00:00:00

abstract：:The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis. As very little is known on the molecular level about mycorrhi...

journal_title：Protoplasma

authors： Menotta M,Amicucci A,Basili G,Rivero F,Polidori E,Sisti D,Stocchi V

更新日期：2007-01-01 00:00:00

abstract：:Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the ...

journal_title：Protoplasma

authors： Han Y,Yu J,Guo F,Watkins SC

更新日期：2006-05-01 00:00:00

abstract：:1. Cells of Schizosaccharomyces pombe from the stationary growth phase show only a low capacity for the uptake of xanthine. In the presence of 2 muM xanthine, the initial uptake rate is found in the range of 6-10- minus 21 Moles times Cells- minus 1 times Min- minus 1 at 30 degrees C. 2. Preincubation in glucose solut...

journal_title：Protoplasma

authors： Seipel S,Reichert U

更新日期：1975-01-01 00:00:00

abstract：:Fluorescence in situ hybridization revealed the presence of ribosomal RNA genes in paracentromeric regions of all A chromosomes and in the distal half of B chromosomes in embryonic cells from Moroccan specimens of the grasshopper Eyprepocnemis plorans. The expression of these genes was monitored by the presence of nuc...

journal_title：Protoplasma

authors： Bakkali M,Cabrero J,López-León MD,Perfectti F,Camacho JP

更新日期：2001-01-01 00:00:00

abstract：:The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

journal_title：Protoplasma

authors： Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

更新日期：2003-09-01 00:00:00

abstract：:Podophyllum hexandrum Royle is known for its vast medicinal properties, particularly anticancer. It contains higher amount of podophyllotoxin (4.3 %), compared to Podophyllum peltatum (0.025 %) and other plant species; as a result, it has been used worldwide in the preparation of various drugs including anticancer, an...

journal_title：Protoplasma

authors： Kumar P,Pal T,Sharma N,Kumar V,Sood H,Chauhan RS

更新日期：2015-09-01 00:00:00

abstract：:19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants prov...

journal_title：Protoplasma

authors： Bringmann G,Wolf K,Meininger M,Rokitta M,Haase A

更新日期：2001-01-01 00:00:00

abstract：:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a ma...

journal_title：Protoplasma

authors： Feki K,Tounsi S,Masmoudi K,Brini F

更新日期：2017-07-01 00:00:00

abstract：:The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...

journal_title：Protoplasma

authors： Braun M,Sievers A

更新日期：1993-01-01 00:00:00

abstract：:Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...

journal_title：Protoplasma

authors： Mursalimov S,Deineko E

更新日期：2017-05-01 00:00:00

abstract：:In addition to small molecules such as sugars and amino acids, phloem sap contains macromolecules, including mRNA and proteins. It is generally assumed that all molecules in the phloem sap are on the move from source to sink, but recent evidence suggests that the macromolecules' direction of movement can be controlled...

journal_title：Protoplasma

authors： Malter D,Wolf S

更新日期：2011-01-01 00:00:00

abstract：:Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...

journal_title：Protoplasma

authors： Goga M,Antreich SJ,Bačkor M,Weckwerth W,Lang I

更新日期：2017-05-01 00:00:00

abstract：:Lemna species are reported to accumulate a variety of metals from contaminated/polluted sites. Cadmium is a nonessential element for plant metabolism. In this work, we aimed to investigate physiological responses to low doses of cadmium (up to 100 microM). From exposure to the lowest Cd concentration (1 microM) to the...

journal_title：Protoplasma

authors： Malec P,Maleva MG,Prasad MN,Strzałka K

更新日期：2010-04-01 00:00:00

abstract：:The terrestrial carnivorous species Utricularia dichotoma is known for a great phenotypic plasticity and unusual vegetative organs. Our investigation on 22 sources/populations revealed that after initiation of a leaf and two bladders on a stolon, a bud was formed in the proximal axil of the leaf, developing into a ros...

journal_title：Protoplasma

authors： Reut MS,Płachno BJ

更新日期：2020-03-01 00:00:00

abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...

journal_title：Protoplasma

authors： Yadav R,Mehrotra M,Singh AK,Niranjan A,Singh R,Sanyal I,Lehri A,Pande V,Amla DV

更新日期：2017-01-01 00:00:00

abstract：:Proper mitochondrial morphogenesis is crucial for successful development of motile sperm. It was known that recessive Drosophila melanogaster mutation emm caused anomalies in the formation of a mitochondrial derivative--nebenkern and led to male sterility. Here we identified primary mutation effect and showed that emm...

journal_title：Protoplasma

authors： Dorogova NV,Bolobolova EU,Akhmetova KA,Fedorova SA

更新日期：2013-04-01 00:00:00

abstract：:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...

journal_title：Protoplasma

authors： Farooq S,Fijen C,Hohlbein J

更新日期：2014-03-01 00:00:00

abstract：:Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...

journal_title：Protoplasma

authors： Goel D,Singh AK,Yadav V,Babbar SB,Bansal KC

更新日期：2010-09-01 00:00:00