Abstract:
:19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants proved CSI to be a valuable technique for mapping fluorinated molecules in plants. Exemplarily using trifluoroacetate as a model compound allowed to select appropriate feeding methods and to optimize both concentration and duration of the application to the plant. The time course of the uptake and distribution of trifluoroacetate was monitored by both 19F imaging and 19F CSI. Fluorinated metabolites formed by uptake of 3-fluoro-3-deoxy-D-glucose were detected with 19F CSI.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Bringmann G,Wolf K,Meininger M,Rokitta M,Haase Adoi
10.1007/BF01306603subject
Has Abstractpub_date
2001-01-01 00:00:00pages
134-43issue
3-4eissn
0033-183Xissn
1615-6102journal_volume
218pub_type
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