In vivo 19F NMR chemical-shift imaging of Ancistrocladus species.

Abstract:

:19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants proved CSI to be a valuable technique for mapping fluorinated molecules in plants. Exemplarily using trifluoroacetate as a model compound allowed to select appropriate feeding methods and to optimize both concentration and duration of the application to the plant. The time course of the uptake and distribution of trifluoroacetate was monitored by both 19F imaging and 19F CSI. Fluorinated metabolites formed by uptake of 3-fluoro-3-deoxy-D-glucose were detected with 19F CSI.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Bringmann G,Wolf K,Meininger M,Rokitta M,Haase A

doi

10.1007/BF01306603

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

134-43

issue

3-4

eissn

0033-183X

issn

1615-6102

journal_volume

218

pub_type

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