Abstract：
:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn (Zea mays L.) roots were isolated by phase-partitioning. The fluidity of PMs was estimated by measurement of fluorescence polarization with 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and 1,6-diphenyl-1,3,5-hexatriene (DPH). The PMs of corn roots were relatively rigid. The hydrophobic part of the lipid bilayer was more fluid than the hydrophilic part. After intercalation of Brij 58 into the lipid bilayer the membrane fluidity changed in a concentration-dependent manner. Treatment with the detergent Brij 58 increased the degree of fluorescence polarization for TMA-DPH, while it decreased it for DPH. This effect was saturated at a detergent-to-protein ratio of 1:4 for both fluorescence probes. Although the biophysical characteristics of the membrane were changed after Brij 58 treatment, the formation of ATP-dependent proton gradients could still be measured with those vesicles. The generation of an ATP-dependent proton gradient with Brij 58-treated PM vesicles suggests that the detergent treatment indeed turned the originally right-side-out vesicles to sealed inside-out vesicles. The limits of the effect caused by Brij 58 in the context of PM enzyme activities are discussed.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Behzadipour M,Kluge M,Lüthje Sdoi
10.1007/BF01289415subject
Has Abstractpub_date
2001-01-01 00:00:00pages
65-9issue
1-3eissn
0033-183Xissn
1615-6102journal_volume
217pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0158-0
更新日期：2010-09-01 00:00:00
abstract：:Salt stress in the rice field is one of the most common abiotic stresses, reducing crop productivity, especially at reproductive stage, which is very sensitive to salt stress. The aim of this investigation was to study mRNA-related Na+ uptake/translocation and Na+ enrichment in the cellular level, leading to physiolog...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01533-w
更新日期：2020-11-01 00:00:00
abstract：:Latex is a complex plant secretion with both ecological and economic importance. There is little information currently available on the cytological aspects of the ontogenesis of anastomosed laticifers, the ducts originating through the lysis of adjacent cell walls. Hancornia speciosa is a tree typical of the Cerrado (...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1262-9
更新日期：2018-11-01 00:00:00
abstract：:Unconventional protein secretion (UPS) is a collective term for mechanisms by which cytosolic proteins that lack a signal peptide ("leaderless secretory proteins" (LSPs)) can gain access to the cell exterior. Numerous examples of UPS have been well documented in animal and yeast cells. In contrast, our understanding o...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-015-0887-1
更新日期：2016-01-01 00:00:00
abstract：:The ultrastructure of the several micrometers thick cytoplasmic layer of the giant marine alga Valonia utricularis displays characteristics which are apparently linked with the capability of this alga to regulate turgor pressure. Transmission and scanning electron microscopy of cells prefixed in different ways, includ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-003-0021-7
更新日期：2003-01-01 00:00:00
abstract：:Despite their great economic importance, relatively little is known about bamboo sexual reproduction because they usually spread through rhizomes and have long intervals between flowering periods. Bambusa tuldoides is no exception; the intervals between flowering periods are about 23 years and often do not result in s...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01479-8
更新日期：2020-05-01 00:00:00
abstract：:The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01415707
更新日期：1999-01-01 00:00:00
abstract：:The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been ma...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-007-0242-2
更新日期：2007-01-01 00:00:00
abstract：:Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0124-4
更新日期：2005-12-01 00:00:00
abstract：:Many studies have been carried out to investigate the histological structure of the trachea in many species of birds. However, the cellular organization of the trachea in the mallard duck is still unclear. This study was performed on 12 sexually mature male Mallard duck to demonstrate the cellular organization of the ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01444-5
更新日期：2020-03-01 00:00:00
abstract：:The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosyn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0804-7
更新日期：2016-03-01 00:00:00
abstract：:Indeterminate growth and the production of new organs in plants require a constant supply of new cells. The majority of these cells are produced in mitotic regions called meristems. For primary or tip growth of the roots and shoots, the meristems are located in the apices. These apical meristems have been shown to fun...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-010-0211-z
更新日期：2010-12-01 00:00:00
abstract：:Pollen development and germination were adversely affected by the presence of mercury, whereas low-concentrations stimulated the whole procedure. Mercury caused morphological anomalies during the tube growth, characterized by irregularly increasing diameters and swelling tips. The main effect was the anomalous cell wa...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1192-y
更新日期：2018-05-01 00:00:00
abstract：:Hemp (Cannabis sativa L., 2n = 20) is a dioecious plant. Sex expression is controlled by an X-to-autosome balance system consisting of the heteromorphic sex chromosomes XY for males and XX for females. Genetically monoecious hemp offers several agronomic advantages compared to the dioecious cultivars that are widely u...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0851-0
更新日期：2016-05-01 00:00:00
abstract：:We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0286-y
更新日期：2008-01-01 00:00:00
abstract：:This micromorphological, chemical and ultrastructural study is a continuation of research conducted on the section Lepidorhiza. The Bulbophyllum echinolabium flowers comprised features that characterize a sapromyophilous syndrome, having large, glistening parts that emit an intense scent of rotten meat. The secretory ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01372-4
更新日期：2019-09-01 00:00:00
abstract：:Catchweed bedstraw is famous for its ability to adhere to other objects due to the presence of numerous trichomes surrounding the stem and mericarps and on the surfaces of the leaves. These trichomes serve as an efficient vector for the movement of the propagules via animals. In this study, we examined the structure a...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0006-7
更新日期：2008-11-01 00:00:00
abstract：:The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1112-1
更新日期：2017-11-01 00:00:00
abstract：:Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0179-x
更新日期：2006-08-01 00:00:00
abstract：:The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0145-5
更新日期：2010-11-01 00:00:00
abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0068-x
更新日期：2003-05-01 00:00:00
abstract：:Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-011-0356-4
更新日期：2012-10-01 00:00:00
abstract：:Narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. The narcissus storage organ was studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually increased in size and reached a maximum at dormancy. In ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0238-3
更新日期：2007-01-01 00:00:00
abstract：:The differentiation of the corneous layers of lizard epidermis has been analyzed by ultrastructural immunocytochemistry using specific antibodies against alpha-keratins and keratin associated beta-proteins (KAbetaPs, formerly indicated as beta-keratins). Both beta-cells and alpha-cells of the corneous layer derive fro...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-013-0585-9
更新日期：2014-07-01 00:00:00
abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0131-5
更新日期：2005-12-01 00:00:00
abstract：:In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher pho...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1010-y
更新日期：2017-03-01 00:00:00
abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0126-2
更新日期：2006-05-01 00:00:00
abstract：:Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0464-9
更新日期：2013-06-01 00:00:00
abstract：:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1214-4
更新日期：2018-07-01 00:00:00
abstract：:Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0157-3
更新日期：2006-09-01 00:00:00
