Isolation and quantification of the heat shock protein 90 alpha and beta isoforms from rat liver.

Abstract:

:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and identified as the Hsp90 alpha and Hsp90 beta isoforms. There was no entry in the protein databases for the Hsp90 alpha isoform from rat. Furthermore, the ratio of the two Hsp90 isoforms was determined.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Langer T,Fasold H

doi

10.1007/BF01288360

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

54-6

issue

1-2

eissn

0033-183X

issn

1615-6102

journal_volume

218

pub_type

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