Critically reduced frost resistance of Picea abies during sprouting could be linked to cytological changes.

abstract：:Understanding the mechanisms that microbes exploit to invade host cells and cause disease is crucial if we are to eliminate their threat. Although pathogens use a variety of microbial factors to trigger entry into non-phagocytic cells, their targeting of the host cell process of endocytosis has emerged as a common the...

journal_title：Protoplasma

authors： Lin AE,Guttman JA

更新日期：2010-08-01 00:00:00

abstract：:The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...

journal_title：Protoplasma

authors： Millaku A,Leser V,Drobne D,Godec M,Torkar M,Jenko M,Milani M,Tatti F

更新日期：2010-05-01 00:00:00

abstract：:The mitotic chromosome structure of 45S rDNA site gaps in Lolium perenne was studied by atomic force microscope (AFM) combining with fluorescence in situ hybridization (FISH) analysis in the present study. FISH on the mitotic chromosomes showed that 45S rDNA gaps were completely broken or local despiralizations of the...

journal_title：Protoplasma

authors： Huang J,Ma L,Sundararajan S,Fei SZ,Li L

更新日期：2009-07-01 00:00:00

abstract：:Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are ...

journal_title：Protoplasma

authors： Mazel T

更新日期：2017-05-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is characterized by multistage formation. The presence of ductular reaction, cytokeratin 7 positivity (PCK7), and increased levels of gamma glutamyltransferase (γGT) has been observed during liver carcinogenesis and contribute to tum...

journal_title：Protoplasma

authors： Moreira AJ,Rodrigues GR,Bona S,Fratta LX,Weber GR,Picada JN,Dos Santos JL,Cerski CT,Marroni CA,Marroni NP

更新日期：2017-03-01 00:00:00

abstract：:Nematocysts are the taxon-defining features of all cnidarians including jellyfish, sea anemones, and corals. They are highly sophisticated organelles used for the capture of prey and defense. The nematocyst capsule is produced within a giant post-Golgi vesicle, which is continuously fed by proteins from the secretory ...

journal_title：Protoplasma

authors： Ozbek S

更新日期：2011-10-01 00:00:00

abstract：:In the vascular plant Arabidopsis thaliana, synthesis of cysteine and its precursors O-acetylserine and sulfide is distributed between the cytosol, chloroplasts, and mitochondria. This compartmentation contributes to regulation of cysteine synthesis. In contrast to Arabidopsis, cysteine synthesis is exclusively restri...

journal_title：Protoplasma

authors： Birke H,Müller SJ,Rother M,Zimmer AD,Hoernstein SN,Wesenberg D,Wirtz M,Krauss GJ,Reski R,Hell R

更新日期：2012-06-01 00:00:00

abstract：:Salt stress in the rice field is one of the most common abiotic stresses, reducing crop productivity, especially at reproductive stage, which is very sensitive to salt stress. The aim of this investigation was to study mRNA-related Na+ uptake/translocation and Na+ enrichment in the cellular level, leading to physiolog...

journal_title：Protoplasma

authors： Theerawitaya C,Samphumphuang T,Tisarum R,Siangliw M,Cha-Um S,Takabe T,Toojinda T

更新日期：2020-11-01 00:00:00

abstract：:Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...

journal_title：Protoplasma

authors： Goel D,Singh AK,Yadav V,Babbar SB,Bansal KC

更新日期：2010-09-01 00:00:00

abstract：:With the goal of evaluating the factors that may be influencing the reproductive success of an endangered, terrestrial orchid Cypripedium japonicum Thunb., the anatomical and morphological characteristics of seed development were assessed, as were the levels of endogenous hormones to investigate the possible relations...

journal_title：Protoplasma

authors： Yan X,Tian M,Liu F,Wang C,Zhang Y

更新日期：2017-11-01 00:00:00

abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

journal_title：Protoplasma

authors： Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

更新日期：2006-08-01 00:00:00

abstract：:The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...

journal_title：Protoplasma

authors： Lütz C

更新日期：2010-08-01 00:00:00

abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

journal_title：Protoplasma

authors： Brown RC,Lemmon BE

更新日期：2009-10-01 00:00:00

abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

journal_title：Protoplasma

authors： Tien CJ,Sigee DC,White KN

更新日期：2005-10-01 00:00:00

abstract：:Approximately 20,000 mammalian genes are estimated to encode between 250 thousand and 1 million different proteins. This enormous diversity of the mammalian proteome is caused by the ability of a single-gene locus to encode multiple protein isoforms. Protein isoforms encoded by one gene locus can be functionally disti...

journal_title：Protoplasma

authors： Vacik T,Raska I

更新日期：2017-05-01 00:00:00

abstract：:Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...

journal_title：Protoplasma

authors： Gens JS,Reuzeau C,Doolittle KW,McNally JG,Pickard BG

更新日期：1996-01-01 00:00:00

abstract：:Fluorescence in situ hybridization revealed the presence of ribosomal RNA genes in paracentromeric regions of all A chromosomes and in the distal half of B chromosomes in embryonic cells from Moroccan specimens of the grasshopper Eyprepocnemis plorans. The expression of these genes was monitored by the presence of nuc...

journal_title：Protoplasma

authors： Bakkali M,Cabrero J,López-León MD,Perfectti F,Camacho JP

更新日期：2001-01-01 00:00:00

abstract：:Salinity is a major stress that adversely affects plant growth and crop production. Understanding the cellular responses and molecular mechanisms by which plants perceive and adopt salinity stress is of fundamental importance. In this work, some of the cellular signaling events including cell death, reactive oxygen sp...

journal_title：Protoplasma

authors： Li Z,Yang H,Wu X,Guo K,Li J

更新日期：2015-05-01 00:00:00

abstract：:Along the past recent years, two-photon excitation (TPE) microscopy has moved from the realms of technical curiosity to be a standard application in many advanced cell biology laboratories. The growing body of literature covered in this review points out the obvious advantages of TPE over any other imaging method base...

journal_title：Protoplasma

authors： Feijó JA,Moreno N

更新日期：2004-03-01 00:00:00

abstract：:Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...

journal_title：Protoplasma

authors： Goga M,Antreich SJ,Bačkor M,Weckwerth W,Lang I

更新日期：2017-05-01 00:00:00

abstract：:Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...

journal_title：Protoplasma

authors： Mursalimov S,Deineko E

更新日期：2017-05-01 00:00:00

abstract：:Proper mitochondrial morphogenesis is crucial for successful development of motile sperm. It was known that recessive Drosophila melanogaster mutation emm caused anomalies in the formation of a mitochondrial derivative--nebenkern and led to male sterility. Here we identified primary mutation effect and showed that emm...

journal_title：Protoplasma

authors： Dorogova NV,Bolobolova EU,Akhmetova KA,Fedorova SA

更新日期：2013-04-01 00:00:00

abstract：:Withanolide is one of the most extensively exploited steroidal lactones, which are biosynthesized in Withania somnifera. Its production from cell suspension culture was analyzed to defeat limitations coupled with its regular supply from the plant organs. In order to optimize the different factors for sustainable produ...

journal_title：Protoplasma

authors： Sivanandhan G,Kapil Dev G,Jeyaraj M,Rajesh M,Muthuselvam M,Selvaraj N,Manickavasagam M,Ganapathi A

更新日期：2013-08-01 00:00:00

abstract：:Titanium dioxide nanoparticles (TiO2-NP) are increasingly being proposed for nanoagriculture but their effect on photosynthesis is limited and contradictory, mostly regarding putative chronical effects associated to the exposure to the commercial P25 formulation (anatase:rutile). This research aims at evaluating how c...

journal_title：Protoplasma

authors： Dias MC,Santos C,Pinto G,Silva AMS,Silva S

更新日期：2019-01-01 00:00:00

abstract：:Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...

journal_title：Protoplasma

authors： Tütüncü Konyar S

更新日期：2017-03-01 00:00:00

abstract：:Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...

journal_title：Protoplasma

authors： Ahanger MA,Tittal M,Mir RA,Agarwal RM

更新日期：2017-09-01 00:00:00

abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...

journal_title：Protoplasma

authors： Yadav R,Mehrotra M,Singh AK,Niranjan A,Singh R,Sanyal I,Lehri A,Pande V,Amla DV

更新日期：2017-01-01 00:00:00

abstract：:Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...

journal_title：Protoplasma

authors： Wang H,Wu Y,Yang X,Guo X,Cao X

更新日期：2017-03-01 00:00:00

abstract：:Some species of Spirogyra form rosette-shaped or rod-shaped rhizoids in the terminal cell of the filaments. In the present study, we analyzed an involvement of microtubules (MTs) in rhizoid differentiation. Before rhizoid differentiation, cortical MTs were arranged transversely to the long axis of cylindrical cells, r...

journal_title：Protoplasma

authors： Yoshida K,Inoue N,Sonobe S,Shimmen T

更新日期：2003-06-01 00:00:00

abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

journal_title：Protoplasma

authors： Felle HH,Herrmann A,Hückelhoven R,Kogel KH

更新日期：2005-12-01 00:00:00