Cytomixis in tobacco microsporogenesis: are there any genome parts predisposed to migration?

Abstract:

:Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question whether cytomixis is a random or a targeted process is answered. The distribution of four repetitive sequences used for identifying the tobacco chromosomes-NTRS, 5S rDNA, GRS, and HSR60-has been examined in the migrating chromatin, and the micronuclei formed after cytomixis. The distribution of tobacco S and T genomes has been analyzed in the cytomictic chromatin using genomic in situ hybridization (GISH). As has been shown (χ 2 test), the labeled DNA probes marking the listed sequences in tobacco genome are observed in the micronuclei formed after cytomixis with the probability not exceeding the theoretically expected value if cytomixis considered as a random process. Thus, it is shown that cytomixis is not a targeted process, and the chromosomes migrate between microsporocytes in a random manner.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Mursalimov S,Deineko E

doi

10.1007/s00709-016-1028-1

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

1379-1384

issue

3

eissn

0033-183X

issn

1615-6102

pii

10.1007/s00709-016-1028-1

journal_volume

254

pub_type

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