Abstract：
:Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This problem was addressed in cultured sycamore (Acer pseudoplatanus L.) cells by comparing the effects of fusicoccin, Tunicamycin and Brefeldin A, inducers of programmed cell death with well-defined molecular and cellular targets, on some of the parameters involved in the regulation of this process. In addition to cell death, the inducers are able to stimulate the production of H2O2, the leakage of cytochrome c from mitochondria, the accumulation of cytosolic 14-3-3 proteins, and changes at the endoplasmic reticulum level, such as accumulation of the molecular chaperone binding protein and modifications in the organelle architecture. Interestingly, no additive effect on any of these parameters is observed when fusicoccin is administered in combination with Tunicamycin or Brefeldin A. Thus, these inducers seem to utilize the same or largely coincident pathways to induce programmed cell death and involvement of the endoplasmic reticulum, in addition to that of mitochondria, appears to be a common step.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Malerba M,Cerana R,Crosti Pdoi
10.1007/s00709-004-0053-7subject
Has Abstractpub_date
2004-10-01 00:00:00pages
61-70issue
1-2eissn
0033-183Xissn
1615-6102journal_volume
224pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence in the interstitium of mouse testis. Additionally, inactivation of the G-coupled membrane estrogen receptor (GPER) in the testis was performed to obtain insight into TC funct...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1305-2
更新日期：2019-03-01 00:00:00
abstract：:Capitate glandular trichomes (CGT) of sunflower, Helianthus annuus, synthesize bioactive sesquiterpene lactones (STLs) within a short period of only a few days during trichome development. In the current project, the subcellular localization of H. annuus germacrene A monooxygenase (HaGAO), a key enzyme of the STL bios...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0823-4
更新日期：2016-03-01 00:00:00
abstract：:The original version of this article unfortunately contains a mistake. ...
journal_title：Protoplasma
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00709-019-01348-4
更新日期：2019-05-01 00:00:00
abstract：:In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1040-5
更新日期：2017-07-01 00:00:00
abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01289415
更新日期：2001-01-01 00:00:00
abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0068-x
更新日期：2003-05-01 00:00:00
abstract：:Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0124-4
更新日期：2005-12-01 00:00:00
abstract：:By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the m...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1121-0
更新日期：2018-01-01 00:00:00
abstract：:Syagrus coronata is an economically important palm tree grown as an ornament, for the oil extracted from its seeds, and the wax from its leaves which has several applications in industry. Silicon biocomposites were analyzed in leaves of S. coronata. Silica bodies were found as extracellular silica masses between the h...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0036-5
更新日期：2002-10-01 00:00:00
abstract：:The LIM domain is an evolutionary conserved double-zinc finger motif found in a variety of proteins exhibiting diverse biological roles. LIM domains have been observed to act as modular protein-binding interfaces mediating protein-protein interactions in the cytoplasm and the nucleus. Interaction of LIM domains with s...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s007090200000
更新日期：2002-02-01 00:00:00
abstract：:Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01556-3
更新日期：2021-01-01 00:00:00
abstract：:Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0158-0
更新日期：2010-09-01 00:00:00
abstract：:Palaeontologically, eubacteria are > 3× older than neomura (eukaryotes, archaebacteria). Cell biology contrasts ancestral eubacterial murein peptidoglycan walls and derived neomuran N-linked glycoprotein coats/walls. Misinterpreting long stems connecting clade neomura to eubacteria on ribosomal sequence trees (plus mi...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-019-01442-7
更新日期：2020-05-01 00:00:00
abstract：:Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-011-0357-3
更新日期：2012-06-01 00:00:00
abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1089-9
更新日期：2017-09-01 00:00:00
abstract：:The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosyn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0804-7
更新日期：2016-03-01 00:00:00
abstract：:Brachypodium distachyon has emerged as a model plant for the improvement of grain crops such as wheat, barley and oats and for understanding basic biological processes to facilitate the development of grasses as superior energy crops. Brachypodium is also the first species of the grass subfamily Pooideae with a sequen...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0689-x
更新日期：2015-03-01 00:00:00
abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF02673874
更新日期：2001-01-01 00:00:00
abstract：:In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher pho...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1010-y
更新日期：2017-03-01 00:00:00
abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01288360
更新日期：2001-01-01 00:00:00
abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-009-0073-4
更新日期：2009-10-01 00:00:00
abstract：:The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-014-0687-z
更新日期：2015-01-01 00:00:00
abstract：:ALTERED MERISTEM PROGRAM 1 (AMP1) encodes a putative glutamate-carboxypeptidase important for plant growth and development. In this study, by comparing the growth of Arabidopsis wild-type, amp1-10 and amp1-13 mutants, and AMP1-GFP/OX2- and AMP1-GFP/OX7-overexpressing seedlings in vitro and in soil, we uncover the role...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01458-z
更新日期：2020-03-01 00:00:00
abstract：:Narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. The narcissus storage organ was studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually increased in size and reached a maximum at dormancy. In ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0238-3
更新日期：2007-01-01 00:00:00
abstract：:The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0875-5
更新日期：2016-07-01 00:00:00
abstract：:Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0150-2
更新日期：2006-05-01 00:00:00
abstract：:The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01415707
更新日期：1999-01-01 00:00:00
abstract：:In addition to small molecules such as sugars and amino acids, phloem sap contains macromolecules, including mRNA and proteins. It is generally assumed that all molecules in the phloem sap are on the move from source to sink, but recent evidence suggests that the macromolecules' direction of movement can be controlled...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0215-8
更新日期：2011-01-01 00:00:00
abstract：:In order to corroborate the hypothesis that variations in the rate of spontaneous ultra-weak photon emission (UPE) from germinating seedlings are related to local variations of the lunisolar tidal force, a series of simultaneous tests was performed using the time courses of UPE collected from three plant species-corn,...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-0947-1
更新日期：2017-01-01 00:00:00
abstract：:Unconventional protein secretion (UPS) is a collective term for mechanisms by which cytosolic proteins that lack a signal peptide ("leaderless secretory proteins" (LSPs)) can gain access to the cell exterior. Numerous examples of UPS have been well documented in animal and yeast cells. In contrast, our understanding o...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-015-0887-1
更新日期：2016-01-01 00:00:00
