Abstract:
:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these cells differs from the GA of higher plants in at least six morphological characteristics. Our results show that scales form in the trans-Golgi network (TGN) and transit the TGN in heretofore unrecognized prosecretory vesicles. Prosecretory vesicles differentiate into secretory vesicles prior to exocytosis of scales to the cell surface. Because prosecretory vesicles are only fragments of TGN cisternae, the classical model of cisternal progression is not a valid mechanism of transport in this alga. TGN transport vesicles are also involved in scale formation; however, the role of tubular connections between cisternae of a single stack-TGN unit is not clear. The relationship of two morphological types of cisternal dilations to a membrane-associated, bottlebrush-shaped macromolecule of novel morphology suggests a new hypothesis for the biogenesis of scales.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Hawkins EK,Lee JJdoi
10.1007/BF02673874subject
Has Abstractpub_date
2001-01-01 00:00:00pages
227-38issue
3-4eissn
0033-183Xissn
1615-6102journal_volume
216pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::The purpose of the main research was to investigate the effects of methyl jasmonate (MeJA) (0.05, 0.25, 0.5, and 2.5 mM) on the pollen germination and tube elongation of Pinus nigra. Total pollen germination rate increased after MeJA treatments while the most enhancement was observed at 0.05-mM MeJA. No germination wa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01539-4
更新日期:2020-11-01 00:00:00
abstract::Despite more than 130 years of research, phloem loading is far from being understood in gymnosperms. In part this is due to the special architecture of their leaves. They differ from angiosperm leaves among others by having a transfusion tissue between bundle sheath and the axial vascular elements. This article review...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0239-0
更新日期:2011-01-01 00:00:00
abstract::Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0998-3
更新日期:2017-03-01 00:00:00
abstract::Octanal is a potential alternative to chemical fungicides in controlling postharvest disease of citrus fruit. In this study, the antifungal activity and the underlying mechanism of octanal against spore germination of Penicillium digitatum, one of the main postharvest pathogens in citrus, were investigated. Results sh...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1046-z
更新日期:2017-07-01 00:00:00
abstract::Fragile sites (FSs) in plants have been described for species like Lolium and other grasses. Whereas in humans FSs were shown to be involved in genome instabilities; the consequences of FSs expression in plants are not known yet. To evaluate whether FSs cause karyotype instabilities, we assessed the frequency of micro...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0942-6
更新日期:2017-01-01 00:00:00
abstract::Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on th...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1102-3
更新日期:2017-11-01 00:00:00
abstract::We investigated the diurnal differences in the innermost surface of tracheid cell walls at various developmental stages from cambium to mature xylem. Cryptomeria japonica saplings were cultivated in a growth chamber with a light cycle set at 14 h of light and 10 h of darkness. Samples were collected from the saplings ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0190-2
更新日期:2006-11-01 00:00:00
abstract::Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0158-0
更新日期:2010-09-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01288360
更新日期:2001-01-01 00:00:00
abstract::The F-actin distribution in caulonemal tip cells of the moss Ceratodon purpureus was examined by rhodamine-phalloidin staining. Gravitropically-growing caulonemal tip cells of the moss possess a distinct alignment of microfilaments (MFs) in their apices. Axially oriented actin bundles run from subapical regions to the...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01276796
更新日期:1995-01-01 00:00:00
abstract::In addition to small molecules such as sugars and amino acids, phloem sap contains macromolecules, including mRNA and proteins. It is generally assumed that all molecules in the phloem sap are on the move from source to sink, but recent evidence suggests that the macromolecules' direction of movement can be controlled...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0215-8
更新日期:2011-01-01 00:00:00
abstract::Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments. This presumption is herein evaluated in three gall systems by immunocytochemical and ultrastructural analyses. The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01431-w
更新日期:2020-01-01 00:00:00
abstract::Salinity is the major environmental constraint that affects legume productivity by inducing oxidative stress. Individually, both silicon (Si) nutrition and mycorrhization have been reported to alleviate salt stress. However, the mechanisms adopted by both in mediating stress responses are poorly understood. Thus, pot ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0892-4
更新日期:2016-09-01 00:00:00
abstract::Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01280307
更新日期:2001-01-01 00:00:00
abstract::Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based auxin biosensors to m...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-014-0616-1
更新日期:2014-09-01 00:00:00
abstract::Although rather inconspicuous, movements are an important adaptive trait of plants. Consequently, light- or gravity-induced movements leading to organ bending have been studied intensively. In the field, however, plant movements often result in organ twisting rather than bending. This study investigates the mechanism ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1247-8
更新日期:2018-09-01 00:00:00
abstract::In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0121-0
更新日期:2010-06-01 00:00:00
abstract::Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0759-8
更新日期:2015-09-01 00:00:00
abstract::Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01556-3
更新日期:2021-01-01 00:00:00
abstract::We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-008-0286-y
更新日期:2008-01-01 00:00:00
abstract::Root hairs are tubular cells resulting from a tip-localized growth in which calcium ions play a key role. Hypaphorine, an indole alkaloid secreted by the fungus Pisolithus microcarpus during the formation of ectomycorrhizae with the host plant Eucalyptus globulus, inhibits root hair tip growth. Hypaphorine-induced inh...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0240-9
更新日期:2007-01-01 00:00:00
abstract::The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0161-5
更新日期:2010-08-01 00:00:00
abstract::Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-013-0596-6
更新日期:2014-03-01 00:00:00
abstract::Tradescantia virginiana leaf epidermal cells were plasmolysed by sequential treatment with 0.8 M and 0.3 M sucrose. Plasmolysis revealed adhesion of the plasma membrane to the cell wall at sites coinciding with cytoskeletal arrays involved in the polarisation of cells undergoing asymmetric divisions--cortical actin pa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01280301
更新日期:2001-01-01 00:00:00
abstract::Nematocysts are the taxon-defining features of all cnidarians including jellyfish, sea anemones, and corals. They are highly sophisticated organelles used for the capture of prey and defense. The nematocyst capsule is produced within a giant post-Golgi vesicle, which is continuously fed by proteins from the secretory ...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0219-4
更新日期:2011-10-01 00:00:00
abstract::Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-011-0356-4
更新日期:2012-10-01 00:00:00
abstract::The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0242-5
更新日期:2011-10-01 00:00:00
abstract::Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1086-z
更新日期:2017-09-01 00:00:00
abstract::This micromorphological, chemical and ultrastructural study is a continuation of research conducted on the section Lepidorhiza. The Bulbophyllum echinolabium flowers comprised features that characterize a sapromyophilous syndrome, having large, glistening parts that emit an intense scent of rotten meat. The secretory ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01372-4
更新日期:2019-09-01 00:00:00
abstract::In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0033-3
更新日期:2004-06-01 00:00:00