Abstract：
:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic ruler, allows for the detection and precise characterisation of dynamic and rarely occurring events, which are otherwise averaged out in ensemble-based experiments. In this review, we will highlight some recent developments that provide new means of studying complex biological systems either by combining smFRET with force-based techniques or by using data obtained from smFRET experiments as constrains for computer-aided modelling.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Farooq S,Fijen C,Hohlbein Jdoi
10.1007/s00709-013-0596-6subject
Has Abstractpub_date
2014-03-01 00:00:00pages
317-32issue
2eissn
0033-183Xissn
1615-6102journal_volume
251pub_type
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