Abstract：
:The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics in wheat (Triticum aestivum L. cv. Kite) root-tip cells were investigated. All three processes were inhibited by the sulfhydryl-modifying agent N-ethylmalemide (NEM), indicating the probable involvement of myosin as the motor protein in each case. However, none of these myosin-dependent processes were inhibited by BDM at concentrations as high as 20 mM in some instances. These results therefore question the general usefulness of BDM as an inhibitor of myosin-based activities in plant cells.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
McCurdy DWdoi
10.1007/BF01415707subject
Has Abstractpub_date
1999-01-01 00:00:00pages
120-5issue
1-2eissn
0033-183Xissn
1615-6102journal_volume
209pub_type
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