Abstract：
:The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis. As very little is known on the molecular level about mycorrhizal morphogenesis and development and these events depend on a tightly regulated reorganisation of the cytoskeleton network in filamentous fungi, we focused on the molecular characterisation of the cdc42 gene in Tuber borchii Vittad., an ascomycetous hypogeous fungus forming ectomycorrhizae. The entire gene was isolated from a T. borchii cDNA library and Southern blot analyses showed that only one copy of cdc42 is present in the T. borchii genome. The predicted amino acid sequence is very similar to those of other known small GTPases and the similar domain structures suggest a similar function. Real-time PCR analyses revealed an increased expression of Tbcdc42 during the phase preparative to the instauration of symbiosis, in particular after stimulation with root exudate extracts. Immunolocalisation experiments revealed an accumulation of CDC42 in the apical tips of the growing hyphae. When a constitutively active Tbcdc42 mutant was expressed in Saccharomyces cerevisiae, morphological changes typical of pseudohyphal growth were observed. Our results suggest a fundamental role of CDC42 in cell polarity development in T. borchii.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Menotta M,Amicucci A,Basili G,Rivero F,Polidori E,Sisti D,Stocchi Vdoi
10.1007/s00709-007-0254-ysubject
Has Abstractpub_date
2007-01-01 00:00:00pages
227-37issue
3-4eissn
0033-183Xissn
1615-6102journal_volume
231pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-0998-3
更新日期：2017-03-01 00:00:00
abstract：:Investigation of 67 gymnosperm and angiosperm species belonging to 25 orders shows that epistomatal mucilage plugs are a widespread phenomenon. Measurements of the leaf water status by using the leaf patch clamp pressure technique suggest that the mucilage plugs are involved in moisture uptake and buffering leaf cells...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0029-0
更新日期：2009-03-01 00:00:00
abstract：:The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosyn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0804-7
更新日期：2016-03-01 00:00:00
abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0092-8
更新日期：2005-10-01 00:00:00
abstract：:Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on th...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1102-3
更新日期：2017-11-01 00:00:00
abstract：:Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0158-0
更新日期：2010-09-01 00:00:00
abstract：:In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by up...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0756-y
更新日期：2015-09-01 00:00:00
abstract：:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0936-9
更新日期：2017-01-01 00:00:00
abstract：:Reactive carbonyl species methylglyoxal (MG) has always been regarded as a cytotoxic metabolite, but now is emerging to function as signal molecule in plants. However, whether MG can induce salt tolerance is elusive. In this study, treatment of wheat seeds with NaCl reduced seed germination, plant height, root length,...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1094-z
更新日期：2017-09-01 00:00:00
abstract：:Developing flowers are important sinks in Arabidopsis thaliana. Their energy demand is covered by assimilates which are synthesized in source leaves and transported via the vasculature. Assimilates are unloaded either symplastically through plasmodesmata or apoplastically by specific transport proteins. Here we studie...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0223-8
更新日期：2011-01-01 00:00:00
abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01279915
更新日期：1985-01-01 00:00:00
abstract：:The LIM domain is an evolutionary conserved double-zinc finger motif found in a variety of proteins exhibiting diverse biological roles. LIM domains have been observed to act as modular protein-binding interfaces mediating protein-protein interactions in the cytoplasm and the nucleus. Interaction of LIM domains with s...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s007090200000
更新日期：2002-02-01 00:00:00
abstract：:Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-011-0357-3
更新日期：2012-06-01 00:00:00
abstract：:Interactive effects of two heavy metal pollutants Cd and Pb in the growth medium were examined on their uptake, production of reactive oxygen species (ROS), induction of oxidative stress and antioxidative defence responses in Indica rice (Oryza sativa L.) seedlings. When rice seedlings in sand culture were exposed to ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0614-3
更新日期：2014-09-01 00:00:00
abstract：:The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0147-5
更新日期：2006-05-01 00:00:00
abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0419-1
更新日期：2013-02-01 00:00:00
abstract：:Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1086-z
更新日期：2017-09-01 00:00:00
abstract：:Here we present an overview of what is known about endogenous plant compounds that act as inhibitors of hormonal transport processes in plants, about their identity and mechanism of action. We have also summarized commonly and less commonly used compounds of non-plant origin and synthetic drugs that show at least part...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-015-0897-z
更新日期：2016-11-01 00:00:00
abstract：:HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemi...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0791-8
更新日期：2015-11-01 00:00:00
abstract：:Iron (Fe) toxicity is one of the major abiotic stresses which limits the yield of lowland rice. This study aims to investigate the physiological, biochemical, and molecular aspects of two contrasting aromatic Joha rice, viz., Keteki and Kola Joha of Assam. Oxidative damage caused due to Fe2+ toxicity was quantitativel...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01574-1
更新日期：2020-10-17 00:00:00
abstract：:The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions i...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0123-5
更新日期：2005-12-01 00:00:00
abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s007090200021
更新日期：2002-05-01 00:00:00
abstract：:The mitotic chromosome structure of 45S rDNA site gaps in Lolium perenne was studied by atomic force microscope (AFM) combining with fluorescence in situ hybridization (FISH) analysis in the present study. FISH on the mitotic chromosomes showed that 45S rDNA gaps were completely broken or local despiralizations of the...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-009-0051-x
更新日期：2009-07-01 00:00:00
abstract：:Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are ...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-017-1075-2
更新日期：2017-05-01 00:00:00
abstract：:Podophyllum species (Podophyllum hexandrum Royle and Podophyllum peltatum) are a major source of deriving anticancer drugs from their major chemical constituent, podophyllotoxin. However, information lacks on regulatory components of podophyllotoxin biosynthesis; therefore, different classes of transcription factors w...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0938-7
更新日期：2017-01-01 00:00:00
abstract：:This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01425-8
更新日期：2020-01-01 00:00:00
abstract：:The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0145-5
更新日期：2010-11-01 00:00:00
abstract：:Fluorescence in situ hybridization revealed the presence of ribosomal RNA genes in paracentromeric regions of all A chromosomes and in the distal half of B chromosomes in embryonic cells from Moroccan specimens of the grasshopper Eyprepocnemis plorans. The expression of these genes was monitored by the presence of nuc...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01283399
更新日期：2001-01-01 00:00:00
abstract：:The cryptomonad nucleomorph is a vestigial nucleus of a eukaryotic red alga engulfed by a phagotrophic protist and retained as a photosynthetic endosymbiont. This review recounts the initial discovery and subsequent characterisation of the cryptomonad nucleomorph focusing on the key role of Peter Sitte and his protégé...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1153-5
更新日期：2017-09-01 00:00:00
abstract：:In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0121-0
更新日期：2010-06-01 00:00:00
