Abstract:
:The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been maintained in a 1 mM Mn-treated environment and again on the young floating laminae after 10 days of further treatment with 1 mM Mn. Mn storing was observed from the first days after germination, but only 10-day-treated laminae showed the capability to hyperaccumulate the element inside specialised cells (>20000 microg/g [dry weight]). Electron microscopy and the Folin-Ciocalteu reaction for phenolics revealed deposits of chelated material inside vacuoles of the first palisade layer and of idioblasts in the spongy tissue. X-ray microanalysis indicated that the deposits were Mn chelated with phenolic compounds. Numerous trichomes were observed at the lower epidermis of 10-day-treated laminae. They were rich in phenolics and characterised by Mn concretions at their base. As they are associated with a high concentration of the metal in culture water and sediments, trichomes may constitute a morphological differentiation for the secretion of Mn-chelating molecules into the culture water, as a probable "avoidance" mechanism. Finally, monitoring of the photosynthetic apparatus showed that photosynthetic function was not impaired, though differences in development occurred.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Baldisserotto C,Ferroni L,Anfuso E,Pagnoni A,Fasulo MP,Pancaldi Sdoi
10.1007/s00709-007-0242-2subject
Has Abstractpub_date
2007-01-01 00:00:00pages
65-82issue
1-2eissn
0033-183Xissn
1615-6102journal_volume
231pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0157-3
更新日期:2006-09-01 00:00:00
abstract::Ascochyta pisi is a necrotrophic pathogenic fungus, which mainly survives between seasons through infected seeds. Defence responses of pea embryo axes to A. pisi were investigated in the heterotrophic phase of seed germination and during the transition from the heterotrophic to the autotrophic phase. Germinated pea se...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-012-0374-x
更新日期:2013-02-01 00:00:00
abstract::It is well established that cytoplasmic microtubules are depolymerized during nuclear division and reassembled as mitotic microtubules. Mounting evidence showing that cytoplasmic microtubules were also involved in apical growth of fungal hyphae posed the question of whether apical growth became disrupted during nuclea...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0020-8
更新日期:2003-01-01 00:00:00
abstract::Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-pa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0180-2
更新日期:2011-04-01 00:00:00
abstract::We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-007-0273-8
更新日期:2007-01-01 00:00:00
abstract::Withanolide is one of the most extensively exploited steroidal lactones, which are biosynthesized in Withania somnifera. Its production from cell suspension culture was analyzed to defeat limitations coupled with its regular supply from the plant organs. In order to optimize the different factors for sustainable produ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-012-0471-x
更新日期:2013-08-01 00:00:00
abstract::Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01289422
更新日期:2001-01-01 00:00:00
abstract::In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0033-3
更新日期:2004-06-01 00:00:00
abstract::We investigated the diurnal differences in the innermost surface of tracheid cell walls at various developmental stages from cambium to mature xylem. Cryptomeria japonica saplings were cultivated in a growth chamber with a light cycle set at 14 h of light and 10 h of darkness. Samples were collected from the saplings ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0190-2
更新日期:2006-11-01 00:00:00
abstract::Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-011-0356-4
更新日期:2012-10-01 00:00:00
abstract::The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions i...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-005-0123-5
更新日期:2005-12-01 00:00:00
abstract::Latex is a complex plant secretion with both ecological and economic importance. There is little information currently available on the cytological aspects of the ontogenesis of anastomosed laticifers, the ducts originating through the lysis of adjacent cell walls. Hancornia speciosa is a tree typical of the Cerrado (...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1262-9
更新日期:2018-11-01 00:00:00
abstract::In addition to small molecules such as sugars and amino acids, phloem sap contains macromolecules, including mRNA and proteins. It is generally assumed that all molecules in the phloem sap are on the move from source to sink, but recent evidence suggests that the macromolecules' direction of movement can be controlled...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0215-8
更新日期:2011-01-01 00:00:00
abstract::Interactive effects of two heavy metal pollutants Cd and Pb in the growth medium were examined on their uptake, production of reactive oxygen species (ROS), induction of oxidative stress and antioxidative defence responses in Indica rice (Oryza sativa L.) seedlings. When rice seedlings in sand culture were exposed to ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-014-0614-3
更新日期:2014-09-01 00:00:00
abstract::We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-005-0131-5
更新日期:2005-12-01 00:00:00
abstract::A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s007090200021
更新日期:2002-05-01 00:00:00
abstract::In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0922-2
更新日期:2017-01-01 00:00:00
abstract::The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-008-0004-9
更新日期:2008-11-01 00:00:00
abstract::The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0004-8
更新日期:2003-09-01 00:00:00
abstract::Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-004-0081-3
更新日期:2005-04-01 00:00:00
abstract::The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Ca(v)1.2), situated on the plasmalemma, in opposition to the type 2 ryanodine receptor (RyR2), situated on the sarcoplasmic reticulum. These two molecules, which form the basis of excitation-contraction coupling, a...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-011-0347-5
更新日期:2012-02-01 00:00:00
abstract::Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0940-0
更新日期:2017-01-01 00:00:00
abstract::The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa-P. palustris, P. belevskii, and P. stagnalis-were studied using light and transmission electron microscopy. We applied the periodic acid-Schiff reaction that is a highly selective method for detecting glycogen. Th...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01546-5
更新日期:2020-11-01 00:00:00
abstract::Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0176-0
更新日期:2006-08-01 00:00:00
abstract::The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a flight parabola, an acceleration profile is achieved which yields...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0209-8
更新日期:2006-12-01 00:00:00
abstract::Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the ...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-013-0604-x
更新日期:2014-03-01 00:00:00
abstract::Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01280307
更新日期:2001-01-01 00:00:00
abstract::DNA and machinery for gene expression have been discovered in chloroplasts during the 1960s. It was soon evident that the chloroplast genome is relatively small, that most genes for chloroplast-localized proteins reside in the nucleus and that chloroplast membranes, ribosomes, and protein complexes are composed of pro...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1104-1
更新日期:2017-09-01 00:00:00
abstract::Phenolics are involved in many of plants' biological functions. In particular, they play important roles in determining the quality of grape berries and the wine made from them, and can also act as antioxidants with beneficial effects for human health. Several enzymes are involved in the synthesis of phenolic compound...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1278-1
更新日期:2019-01-01 00:00:00
abstract::In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0936-9
更新日期:2017-01-01 00:00:00