Melon phloem-sap proteome: developmental control and response to viral infection.

abstract：:Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the ...

journal_title：Protoplasma

authors： Wachsmuth M

更新日期：2014-03-01 00:00:00

abstract：:DNA and machinery for gene expression have been discovered in chloroplasts during the 1960s. It was soon evident that the chloroplast genome is relatively small, that most genes for chloroplast-localized proteins reside in the nucleus and that chloroplast membranes, ribosomes, and protein complexes are composed of pro...

journal_title：Protoplasma

authors： Börner T

更新日期：2017-09-01 00:00:00

abstract：:The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...

journal_title：Protoplasma

authors： Kościńska-Pajak M,Bednara J

更新日期：2006-05-01 00:00:00

abstract：:The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...

journal_title：Protoplasma

authors： Liu L,Hua BZ

更新日期：2018-07-01 00:00:00

abstract：:Podophyllum hexandrum Royle is known for its vast medicinal properties, particularly anticancer. It contains higher amount of podophyllotoxin (4.3 %), compared to Podophyllum peltatum (0.025 %) and other plant species; as a result, it has been used worldwide in the preparation of various drugs including anticancer, an...

journal_title：Protoplasma

authors： Kumar P,Pal T,Sharma N,Kumar V,Sood H,Chauhan RS

更新日期：2015-09-01 00:00:00

abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

journal_title：Protoplasma

authors： Felle HH,Herrmann A,Hückelhoven R,Kogel KH

更新日期：2005-12-01 00:00:00

abstract：:Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...

journal_title：Protoplasma

authors： Sobol M,Gonzalez-Camacho F,Rodríguez-Vilariño V,Kordyum E,Medina FJ

更新日期：2006-09-01 00:00:00

abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

journal_title：Protoplasma

authors： Brown RC,Lemmon BE

更新日期：2009-10-01 00:00:00

abstract：:Catchweed bedstraw is famous for its ability to adhere to other objects due to the presence of numerous trichomes surrounding the stem and mericarps and on the surfaces of the leaves. These trichomes serve as an efficient vector for the movement of the propagules via animals. In this study, we examined the structure a...

journal_title：Protoplasma

authors： Bowling AJ,Maxwell HB,Vaughn KC

更新日期：2008-11-01 00:00:00

abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...

journal_title：Protoplasma

authors： Hawkins EK,Lee JJ

更新日期：2001-01-01 00:00:00

abstract：:In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...

journal_title：Protoplasma

authors： Bist R,Misra S,Bhatt DK

更新日期：2010-06-01 00:00:00

abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

journal_title：Protoplasma

authors： Tien CJ,Sigee DC,White KN

更新日期：2005-10-01 00:00:00

abstract：:This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...

journal_title：Protoplasma

authors： Giannoutsou E,Sotiriou P,Nikolakopoulou TL,Galatis B,Apostolakos P

更新日期：2020-01-01 00:00:00

abstract：:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a ma...

journal_title：Protoplasma

authors： Feki K,Tounsi S,Masmoudi K,Brini F

更新日期：2017-07-01 00:00:00

abstract：:In the genus Genlisea as well as in its sister genus Utricularia, the palate probably plays a key role in providing the colour, mechanical and olfactory stimuli to attract insect pollinators and to guide them to the generative structures and the nectary spur. However, information about the micro-morphology of the pala...

journal_title：Protoplasma

authors： Płachno BJ,Świątek P,Stpiczyńska M,Miranda VFO

更新日期：2018-07-01 00:00:00

abstract：:Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...

journal_title：Protoplasma

authors： Mursalimov S,Deineko E

更新日期：2017-05-01 00:00:00

abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

journal_title：Protoplasma

authors： Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

更新日期：2006-08-01 00:00:00

abstract：:Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...

journal_title：Protoplasma

authors： Singla P,Bhardwaj RD,Kaur S,Kaur J

更新日期：2020-01-01 00:00:00

abstract：:Understanding the mechanisms that microbes exploit to invade host cells and cause disease is crucial if we are to eliminate their threat. Although pathogens use a variety of microbial factors to trigger entry into non-phagocytic cells, their targeting of the host cell process of endocytosis has emerged as a common the...

journal_title：Protoplasma

authors： Lin AE,Guttman JA

更新日期：2010-08-01 00:00:00

abstract：:While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...

journal_title：Protoplasma

authors： Kłopocka W,Redowicz MJ

更新日期：2003-03-01 00:00:00

abstract：:In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...

journal_title：Protoplasma

authors： Kanu SA,Dakora FD

更新日期：2017-01-01 00:00:00

abstract：:Syagrus coronata is an economically important palm tree grown as an ornament, for the oil extracted from its seeds, and the wax from its leaves which has several applications in industry. Silicon biocomposites were analyzed in leaves of S. coronata. Silica bodies were found as extracellular silica masses between the h...

journal_title：Protoplasma

authors： Lins U,Barros CF,da Cunha M,Miguens FC

更新日期：2002-10-01 00:00:00

abstract：:Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...

journal_title：Protoplasma

authors： Wang H,Wu Y,Yang X,Guo X,Cao X

更新日期：2017-03-01 00:00:00

abstract：:In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...

journal_title：Protoplasma

authors： Lütz-Meindl U,Aichinger N

更新日期：2004-06-01 00:00:00

abstract：:We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...

journal_title：Protoplasma

authors： Forer A,Spurck T,Pickett-Heaps JD

更新日期：2007-01-01 00:00:00

abstract：:Flagellar development in the plurilocular zoidangia of sporophytes of the brown alga Ectocarpus siliculosus was analyzed in detail using transmission electron microscopy and electron tomography. A series of cell divisions in the plurilocular zoidangia produced the spore-mother cells. In these cells, the centrioles dif...

journal_title：Protoplasma

authors： Fu G,Nagasato C,Ito T,Müller DG,Motomura T

更新日期：2013-02-01 00:00:00

abstract：:Vacuoles are ubiquitous organelles in the fungal and plant kingdoms. They serve a variety of functions and are important for cell homeostasis. A constant turnover of proteins and membranes makes vacuoles dynamic organelles. Various transport pathways share the vacuole as their joint destination. The trafficking pathwa...

journal_title：Protoplasma

authors： Götte M,Lazar T

更新日期：1999-01-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:Along the past recent years, two-photon excitation (TPE) microscopy has moved from the realms of technical curiosity to be a standard application in many advanced cell biology laboratories. The growing body of literature covered in this review points out the obvious advantages of TPE over any other imaging method base...

journal_title：Protoplasma

authors： Feijó JA,Moreno N

更新日期：2004-03-01 00:00:00

abstract：:Vicia barbazitae, a taxon belonging to section Vicia of subgenus Vicia, was recovered and analysed by cytological, karyological and molecular methods with the aim of both proposing a general characterisation of this species and studying the relationships among the species of section Vicia . Phy...

journal_title：Protoplasma

authors： Ruffini Castiglione M,Frediani M,Gelati MT,Venora G,Giorgetti L,Caputo P,Cremonini R

更新日期：2012-07-01 00:00:00