Abstract：
:Vicia barbazitae, a taxon belonging to section Vicia of subgenus Vicia, was recovered and analysed by cytological, karyological and molecular methods with the aim of both proposing a general characterisation of this species and studying the relationships among the species of section Vicia . Phylogenetic relationships among the species of the section Vicia and those of the sections Microcarinae, Wiggersia and Atossa were also analysed. Automated karyotype analysis has been determined after Feulgen's reaction; chromosome banding was performed by sequence-specific fluorochrome staining. Fluorescent chromosome banding showed CMA(+)/DAPI(-) NOR-associated heterochromatin in the satellite pair. Karyomorphological parameters, based on symmetry indices, the dendrogram of linkage distance constructed on 37 chromosome parameters, as well as the molecular data based on internal transcribed spacer sequences provided information about phylogenetic position of this species inside the section Vicia and among the species belonging to the sections Microcarinae, Wiggersia, Atossa and Vicia. From our karyological and molecular results, it emerges that V. barbazitae can be considered a natural member of section Vicia.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Ruffini Castiglione M,Frediani M,Gelati MT,Venora G,Giorgetti L,Caputo P,Cremonini Rdoi
10.1007/s00709-011-0323-0subject
Has Abstractpub_date
2012-07-01 00:00:00pages
779-88issue
3eissn
0033-183Xissn
1615-6102journal_volume
249pub_type
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