Chromosomes selectively detach at one pole and quickly move towards the opposite pole when kinetochore microtubules are depolymerized in Mesostoma ehrenbergii spermatocytes.

Abstract:

:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase plate. In our experiments, nocodazole (NOC) was added to prometaphase spermatocytes to depolymerize the microtubules. Traditional theories state that microtubules are the producers of force in the spindle, either by tubulin depolymerizing at the kinetochore (PacMan) or at the pole (Flux). Accordingly, if microtubules are quickly depolymerized, the chromosomes should arrest at the metaphase plate and not move. However, in 57/59 cells, at least one chromosome moved to a pole after NOC treatment, and in 52 of these cells, all three bivalents moved to the same pole. Thus, the movements are not random to one pole or other. After treatment with NOC, chromosome movement followed a consistent pattern. Bivalents stretched out towards both poles, paused, detached at one pole, and then the detached kinetochores quickly moved towards the other pole, reaching initial speeds up to more than 200 μm/min, much greater than anything previously recorded in this cell. As the NOC concentration increased, the average speeds increased and the microtubules disappeared faster. As the kinetochores approached the pole, they slowed down and eventually stopped. Similar results were obtained with colcemid treatment. Confocal immunofluorescence microscopy confirms that microtubules are not associated with moving chromosomes. Thus, these rapid chromosome movements may be due to non-microtubule spindle components such as actin-myosin or the spindle matrix.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Fegaras E,Forer A

doi

10.1007/s00709-018-1214-4

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

1205-1224

issue

4

eissn

0033-183X

issn

1615-6102

pii

10.1007/s00709-018-1214-4

journal_volume

255

pub_type

杂志文章
  • Circadian clock-dependent gating in ABA signalling networks.

    abstract::Plant growth and development are intimately attuned to fluctuations in environmental variables such as light, temperature and water availability. A broad range of signalling and dynamic response mechanisms allows them to adjust their physiology so that growth and reproductive capacity are optimised for the prevailing ...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-011-0304-3

    authors: Seung D,Risopatron JP,Jones BJ,Marc J

    更新日期:2012-07-01 00:00:00

  • Cell physiology of plants growing in cold environments.

    abstract::The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-010-0161-5

    authors: Lütz C

    更新日期:2010-08-01 00:00:00

  • Contribution of the actomyosin motor to the temperature-dependent translational diffusion of water by cytoplasmic streaming in Elodea canadensis cells.

    abstract::The extent to which the actomyosin motor responsible for cytoplasmic streaming contributes to the translational diffusion of water in Elodea canadensis cells was studied by a nuclear magnetic resonance (NMR) spin-echo technique. The relative contribution of the actomyosin motor was determined from the corresponding ap...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-004-0067-1

    authors: Vorob'ev VN,Anisimov AV,Dautova NR

    更新日期:2004-12-01 00:00:00

  • Morphological and ultrastructural characterization of the acidophilic and lipid-producer strain Chlamydomonas acidophila LAFIC-004 (Chlorophyta) under different culture conditions.

    abstract::Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1030-7

    authors: Souza LD,Simioni C,Bouzon ZL,Schneider RC,Gressler P,Miotto MC,Rossi MJ,Rörig LR

    更新日期:2017-05-01 00:00:00

  • What generates flux of tubulin in kinetochore microtubules?

    abstract::We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-008-0286-y

    authors: Forer A,Pickett-Heaps JD,Spurck T

    更新日期:2008-01-01 00:00:00

  • Multivesicular bodies differentiate exclusively in nutritive fast-dividing cells in Marcetia taxifolia galls.

    abstract::Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0759-8

    authors: Ferreira BG,Carneiro RG,Isaias RM

    更新日期:2015-09-01 00:00:00

  • ALTERED MERISTEM PROGRAM 1 promotes growth and biomass accumulation influencing guard cell aperture and photosynthetic efficiency in Arabidopsis.

    abstract::ALTERED MERISTEM PROGRAM 1 (AMP1) encodes a putative glutamate-carboxypeptidase important for plant growth and development. In this study, by comparing the growth of Arabidopsis wild-type, amp1-10 and amp1-13 mutants, and AMP1-GFP/OX2- and AMP1-GFP/OX7-overexpressing seedlings in vitro and in soil, we uncover the role...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01458-z

    authors: López-García CM,Ruíz-Herrera LF,López-Bucio JS,Huerta-Venegas PI,Peña-Uribe CA,de la Cruz HR,López-Bucio J

    更新日期:2020-03-01 00:00:00

  • Isolation and characterisation of two wheat beta-expansin genes expressed during male gametophyte development.

    abstract::Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-006-0176-0

    authors: Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

    更新日期:2006-08-01 00:00:00

  • Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae.

    abstract::Endoplasmic reticulum (ER) is a major site for secretory protein folding and lipid synthesis. Since ER cannot be synthesized de novo, it must be inherited during the cell cycle. Studying ER inheritance can however be difficult because the ER of typical plant and animal cells is morphologically complex. Therefore, our ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-011-0359-1

    authors: Yagisawa F,Fujiwara T,Kuroiwa H,Nishida K,Imoto Y,Kuroiwa T

    更新日期:2012-10-01 00:00:00

  • Ontogeny of anastomosed laticifers in the stem apex of Hancornia speciosa (Apocynaceae): a topographic approach.

    abstract::Latex is a complex plant secretion with both ecological and economic importance. There is little information currently available on the cytological aspects of the ontogenesis of anastomosed laticifers, the ducts originating through the lysis of adjacent cell walls. Hancornia speciosa is a tree typical of the Cerrado (...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-018-1262-9

    authors: Gonçalves MP,Mercadante-Simões MO,Ribeiro LM

    更新日期:2018-11-01 00:00:00

  • Flower palate ultrastructure of the carnivorous plant Genlisea hispidula Stapf with remarks on the structure and function of the palate in the subgenus Genlisea (Lentibulariaceae).

    abstract::In the genus Genlisea as well as in its sister genus Utricularia, the palate probably plays a key role in providing the colour, mechanical and olfactory stimuli to attract insect pollinators and to guide them to the generative structures and the nectary spur. However, information about the micro-morphology of the pala...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-018-1220-6

    authors: Płachno BJ,Świątek P,Stpiczyńska M,Miranda VFO

    更新日期:2018-07-01 00:00:00

  • The cryptomonad nucleomorph.

    abstract::The cryptomonad nucleomorph is a vestigial nucleus of a eukaryotic red alga engulfed by a phagotrophic protist and retained as a photosynthetic endosymbiont. This review recounts the initial discovery and subsequent characterisation of the cryptomonad nucleomorph focusing on the key role of Peter Sitte and his protégé...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1153-5

    authors: McFadden GI

    更新日期:2017-09-01 00:00:00

  • Responses of Trapa natans L. floating laminae to high concentrations of manganese.

    abstract::The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been ma...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-007-0242-2

    authors: Baldisserotto C,Ferroni L,Anfuso E,Pagnoni A,Fasulo MP,Pancaldi S

    更新日期:2007-01-01 00:00:00

  • The relevance of compartmentation for cysteine synthesis in phototrophic organisms.

    abstract::In the vascular plant Arabidopsis thaliana, synthesis of cysteine and its precursors O-acetylserine and sulfide is distributed between the cytosol, chloroplasts, and mitochondria. This compartmentation contributes to regulation of cysteine synthesis. In contrast to Arabidopsis, cysteine synthesis is exclusively restri...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-012-0411-9

    authors: Birke H,Müller SJ,Rother M,Zimmer AD,Hoernstein SN,Wesenberg D,Wirtz M,Krauss GJ,Reski R,Hell R

    更新日期:2012-06-01 00:00:00

  • Surface characteristics of isopod digestive gland epithelium studied by SEM.

    abstract::The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-010-0110-3

    authors: Millaku A,Leser V,Drobne D,Godec M,Torkar M,Jenko M,Milani M,Tatti F

    更新日期:2010-05-01 00:00:00

  • Ultrastructure of the larval Malpighian tubules in Terrobittacus implicatus (Mecoptera: Bittacidae).

    abstract::The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-018-1221-5

    authors: Liu L,Hua BZ

    更新日期:2018-07-01 00:00:00

  • The ins and outs of yeast vacuole trafficking.

    abstract::Vacuoles are ubiquitous organelles in the fungal and plant kingdoms. They serve a variety of functions and are important for cell homeostasis. A constant turnover of proteins and membranes makes vacuoles dynamic organelles. Various transport pathways share the vacuole as their joint destination. The trafficking pathwa...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01415696

    authors: Götte M,Lazar T

    更新日期:1999-01-01 00:00:00

  • Molecular cytogenetic analysis of monoecious hemp (Cannabis sativa L.) cultivars reveals its karyotype variations and sex chromosomes constitution.

    abstract::Hemp (Cannabis sativa L., 2n = 20) is a dioecious plant. Sex expression is controlled by an X-to-autosome balance system consisting of the heteromorphic sex chromosomes XY for males and XX for females. Genetically monoecious hemp offers several agronomic advantages compared to the dioecious cultivars that are widely u...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0851-0

    authors: Razumova OV,Alexandrov OS,Divashuk MG,Sukhorada TI,Karlov GI

    更新日期:2016-05-01 00:00:00

  • Androgenic response to preculture stress in microspore cultures of barley.

    abstract::Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-006-0179-x

    authors: Oleszczuk S,Sowa S,Zimny J

    更新日期:2006-08-01 00:00:00

  • Cellular redox state and activating protein-1 are involved in ascorbate effect on calcitriol-induced differentiation.

    abstract::Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01289422

    authors: López-Lluch G,Blázquez MV,Pérez-Vicente R,Macho A,Burón MI,Alcaín FJ,Muñoz E,Navas P

    更新日期:2001-01-01 00:00:00

  • Immunological evidence for the presence of plant homologues of the actin- related protein Arp3 in tobacco and maize: subcellular localization to actin-enriched pit fields and emerging root hairs.

    abstract::The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-003-0004-8

    authors: Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

    更新日期:2003-09-01 00:00:00

  • Critically reduced frost resistance of Picea abies during sprouting could be linked to cytological changes.

    abstract::Frost resistance of sprouting Picea abies shoots is insufficient for survival of naturally occurring late frosts. The cellular changes during sprouting appeared to be responsible for frost damage as frost events that damaged sprouting shoots did not damage older needles and stems. Whilst resting buds showed initial fr...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-009-0052-9

    authors: Neuner G,Beikircher B

    更新日期:2010-07-01 00:00:00

  • Viviparity in the dermapteran Arixenia esau: respiration inside mother's body requires both maternal and larval contribution.

    abstract::Earwigs (Dermaptera) use different strategies to increase their reproductive success. Most species lay eggs; however, viviparity of the matrotrophic type has been reported in two groups: Hemimeridae and Arixeniidae. In Arixeniidae, offspring develop in two separate places: inside an ovary (the intraovarian phase) and ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01402-1

    authors: Jaglarz MK,Tworzydlo W,Rak A,Kotula-Balak M,Sekula M,Bilinski SM

    更新日期:2019-11-01 00:00:00

  • Expression analysis of biosynthetic pathway genes vis-à-vis podophyllotoxin content in Podophyllum hexandrum Royle.

    abstract::Podophyllum hexandrum Royle is known for its vast medicinal properties, particularly anticancer. It contains higher amount of podophyllotoxin (4.3 %), compared to Podophyllum peltatum (0.025 %) and other plant species; as a result, it has been used worldwide in the preparation of various drugs including anticancer, an...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0757-x

    authors: Kumar P,Pal T,Sharma N,Kumar V,Sood H,Chauhan RS

    更新日期:2015-09-01 00:00:00

  • Effect of methyl jasmonate on in-vitro pollen germination and tube elongation of Pinus nigra.

    abstract::The purpose of the main research was to investigate the effects of methyl jasmonate (MeJA) (0.05, 0.25, 0.5, and 2.5 mM) on the pollen germination and tube elongation of Pinus nigra. Total pollen germination rate increased after MeJA treatments while the most enhancement was observed at 0.05-mM MeJA. No germination wa...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01539-4

    authors: Çetinbaş-Genç A,Vardar F

    更新日期:2020-11-01 00:00:00

  • The dynamic roles of intracellular lipid droplets: from archaea to mammals.

    abstract::During the past decade, there has been a paradigm shift in our understanding of the roles of intracellular lipid droplets (LDs). New genetic, biochemical and imaging technologies have underpinned these advances, which are revealing much new information about these dynamic organelles. This review takes a comparative ap...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-011-0329-7

    authors: Murphy DJ

    更新日期:2012-07-01 00:00:00

  • Comparison between the effects of fusicoccin, Tunicamycin, and Brefeldin A on programmed cell death of cultured sycamore (Acer pseudoplatanus L.) cells.

    abstract::Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-004-0053-7

    authors: Malerba M,Cerana R,Crosti P

    更新日期:2004-10-01 00:00:00

  • Diurnal differences in the supply of glucomannans and xylans to innermost surface of cell walls at various developmental stages from cambium to mature xylem in Cryptomeria japonica.

    abstract::We investigated the diurnal differences in the innermost surface of tracheid cell walls at various developmental stages from cambium to mature xylem. Cryptomeria japonica saplings were cultivated in a growth chamber with a light cycle set at 14 h of light and 10 h of darkness. Samples were collected from the saplings ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-006-0190-2

    authors: Hosoo Y,Imai T,Yoshida M

    更新日期:2006-11-01 00:00:00

  • Interactive effects of silicon and arbuscular mycorrhiza in modulating ascorbate-glutathione cycle and antioxidant scavenging capacity in differentially salt-tolerant Cicer arietinum L. genotypes subjected to long-term salinity.

    abstract::Salinity is the major environmental constraint that affects legume productivity by inducing oxidative stress. Individually, both silicon (Si) nutrition and mycorrhization have been reported to alleviate salt stress. However, the mechanisms adopted by both in mediating stress responses are poorly understood. Thus, pot ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0892-4

    authors: Garg N,Bhandari P

    更新日期:2016-09-01 00:00:00

  • Expression level of Na+ homeostasis-related genes and salt-tolerant abilities in backcross introgression lines of rice crop under salt stress at reproductive stage.

    abstract::Salt stress in the rice field is one of the most common abiotic stresses, reducing crop productivity, especially at reproductive stage, which is very sensitive to salt stress. The aim of this investigation was to study mRNA-related Na+ uptake/translocation and Na+ enrichment in the cellular level, leading to physiolog...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01533-w

    authors: Theerawitaya C,Samphumphuang T,Tisarum R,Siangliw M,Cha-Um S,Takabe T,Toojinda T

    更新日期:2020-11-01 00:00:00