Abstract：
:We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules that are associated with a stationary spindle matrix. We present a different viewpoint, that microtubules are propelled poleward by forces arising from the spindle matrix, that the forces on the microtubules "activate" polymerising and depolymerising enzymes at kinetochores and poles, that matrix forces utilise actin, myosin, and microtubule motors, and that the matrix itself may not necessarily be static.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Forer A,Pickett-Heaps JD,Spurck Tdoi
10.1007/s00709-008-0286-ysubject
Has Abstractpub_date
2008-01-01 00:00:00pages
137-41issue
3-4eissn
0033-183Xissn
1615-6102journal_volume
232pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0936-9
更新日期：2017-01-01 00:00:00
abstract：:In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont "Candidatus Sulcia muelleri" (Phylum Bacteroidetes) resides in bacterio...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1033-4
更新日期：2017-05-01 00:00:00
abstract：:Podophyllum hexandrum Royle is known for its vast medicinal properties, particularly anticancer. It contains higher amount of podophyllotoxin (4.3 %), compared to Podophyllum peltatum (0.025 %) and other plant species; as a result, it has been used worldwide in the preparation of various drugs including anticancer, an...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0757-x
更新日期：2015-09-01 00:00:00
abstract：:The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Ca(v)1.2), situated on the plasmalemma, in opposition to the type 2 ryanodine receptor (RyR2), situated on the sarcoplasmic reticulum. These two molecules, which form the basis of excitation-contraction coupling, a...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-011-0347-5
更新日期：2012-02-01 00:00:00
abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0419-1
更新日期：2013-02-01 00:00:00
abstract：:We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-007-0273-8
更新日期：2007-01-01 00:00:00
abstract：:The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a flight parabola, an acceleration profile is achieved which yields...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0209-8
更新日期：2006-12-01 00:00:00
abstract：:The plant root tip represents a fascinating model system for studying changes in Golgi stack architecture associated with the developmental progression of meristematic cells to gravity sensing columella cells, and finally to "young" and "old", polysaccharide-slime secreting peripheral cells. To this end we have used ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01322640
更新日期：1990-01-01 00:00:00
abstract：:The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0145-5
更新日期：2010-11-01 00:00:00
abstract：:Yr36 is an important gene conferring resistance to stripe rust of wheat caused by Puccinia striiformis f. sp. tritici (Pst). To determine if the Yr36 resistance is correlated to reactive oxygen species (ROS) burst and cell death, wheat near-isogenic lines with (UC1041 + Yr36) and without (UC1041) the gene were histolo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0833-2
更新日期：2016-05-01 00:00:00
abstract：:Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01289422
更新日期：2001-01-01 00:00:00
abstract：:Iron (Fe) toxicity is one of the major abiotic stresses which limits the yield of lowland rice. This study aims to investigate the physiological, biochemical, and molecular aspects of two contrasting aromatic Joha rice, viz., Keteki and Kola Joha of Assam. Oxidative damage caused due to Fe2+ toxicity was quantitativel...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01574-1
更新日期：2020-10-17 00:00:00
abstract：:Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-0998-3
更新日期：2017-03-01 00:00:00
abstract：:Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01882029
更新日期：1996-01-01 00:00:00
abstract：:The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0004-9
更新日期：2008-11-01 00:00:00
abstract：:Cytoplasmic streaming is essential for intracellular communications but its specific functions are not well known. In Chara corallina internodes, long-distance interactions mediated by cyclosis are clearly evident with microscopy-pulse amplitude modulation (PAM) fluorometer under application of localized light (LL) pu...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-0975-x
更新日期：2017-01-01 00:00:00
abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01279915
更新日期：1985-01-01 00:00:00
abstract：:Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01392-0
更新日期：2019-11-01 00:00:00
abstract：:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1214-4
更新日期：2018-07-01 00:00:00
abstract：:Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1057-9
更新日期：2017-09-01 00:00:00
abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0940-0
更新日期：2017-01-01 00:00:00
abstract：:The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-014-0687-z
更新日期：2015-01-01 00:00:00
abstract：:The mitotic chromosome structure of 45S rDNA site gaps in Lolium perenne was studied by atomic force microscope (AFM) combining with fluorescence in situ hybridization (FISH) analysis in the present study. FISH on the mitotic chromosomes showed that 45S rDNA gaps were completely broken or local despiralizations of the...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-009-0051-x
更新日期：2009-07-01 00:00:00
abstract：:Many studies have been carried out to investigate the histological structure of the trachea in many species of birds. However, the cellular organization of the trachea in the mallard duck is still unclear. This study was performed on 12 sexually mature male Mallard duck to demonstrate the cellular organization of the ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01444-5
更新日期：2020-03-01 00:00:00
abstract：:Catchweed bedstraw is famous for its ability to adhere to other objects due to the presence of numerous trichomes surrounding the stem and mericarps and on the surfaces of the leaves. These trichomes serve as an efficient vector for the movement of the propagules via animals. In this study, we examined the structure a...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0006-7
更新日期：2008-11-01 00:00:00
abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0068-x
更新日期：2003-05-01 00:00:00
abstract：:Octanal is a potential alternative to chemical fungicides in controlling postharvest disease of citrus fruit. In this study, the antifungal activity and the underlying mechanism of octanal against spore germination of Penicillium digitatum, one of the main postharvest pathogens in citrus, were investigated. Results sh...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1046-z
更新日期：2017-07-01 00:00:00
abstract：:Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a nove...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0684-2
更新日期：2015-01-01 00:00:00
abstract：:Brachypodium distachyon has emerged as a model plant for the improvement of grain crops such as wheat, barley and oats and for understanding basic biological processes to facilitate the development of grasses as superior energy crops. Brachypodium is also the first species of the grass subfamily Pooideae with a sequen...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0689-x
更新日期：2015-03-01 00:00:00
abstract：:In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth reg...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01588-9
更新日期：2020-11-27 00:00:00
