Improvement in Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) by the inhibition of polyphenolics released during wounding of cotyledonary node explants.

abstract：:Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...

journal_title：Protoplasma

authors： Alla MM,Hassan NM

更新日期：2012-10-01 00:00:00

abstract：:19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants prov...

journal_title：Protoplasma

authors： Bringmann G,Wolf K,Meininger M,Rokitta M,Haase A

更新日期：2001-01-01 00:00:00

abstract：:The present study investigated the impact of 1800-MHz electromagnetic field radiations (EMF-r), widely used in mobile communication, on the growth and activity of starch-, sucrose-, and phosphate-hydrolyzing enzymes in Zea mays seedlings. We exposed Z. mays to modulated continuous wave homogenous EMF-r at specific abs...

journal_title：Protoplasma

authors： Kumar A,Singh HP,Batish DR,Kaur S,Kohli RK

更新日期：2016-07-01 00:00:00

abstract：:In this study, the filamentous green alga Zygogonium ericetorum (Zygnematales, Chlorophyta), collected at its natural habitat in the high alps, was investigated by light, scanning, and transmission electron microscopy. The field samples were separated into a moist fraction when wetted by splattering water of a nearby ...

journal_title：Protoplasma

authors： Holzinger A,Tschaikner A,Remias D

更新日期：2010-07-01 00:00:00

abstract：:Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the ...

journal_title：Protoplasma

authors： Han Y,Yu J,Guo F,Watkins SC

更新日期：2006-05-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...

journal_title：Protoplasma

authors： Koutalianou M,Orfanidis S,Katsaros C

更新日期：2016-03-01 00:00:00

abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...

journal_title：Protoplasma

authors： Hensel W

更新日期：1985-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is characterized by multistage formation. The presence of ductular reaction, cytokeratin 7 positivity (PCK7), and increased levels of gamma glutamyltransferase (γGT) has been observed during liver carcinogenesis and contribute to tum...

journal_title：Protoplasma

authors： Moreira AJ,Rodrigues GR,Bona S,Fratta LX,Weber GR,Picada JN,Dos Santos JL,Cerski CT,Marroni CA,Marroni NP

更新日期：2017-03-01 00:00:00

abstract：:Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...

journal_title：Protoplasma

authors： López-Lluch G,Blázquez MV,Pérez-Vicente R,Macho A,Burón MI,Alcaín FJ,Muñoz E,Navas P

更新日期：2001-01-01 00:00:00

abstract：:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...

journal_title：Protoplasma

authors： Zienkiewicz M,Krupnik T,Drożak A,Golke A,Romanowska E

更新日期：2017-01-01 00:00:00

abstract：:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...

journal_title：Protoplasma

authors： Fegaras E,Forer A

更新日期：2018-07-01 00:00:00

abstract：:To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, ...

journal_title：Protoplasma

authors： Sun X,Lian H,Liu X,Zhou S,Liu S

更新日期：2017-05-01 00:00:00

abstract：:Turions, which are modified shoot apices, are vegetative, dormant overwintering organs produced by perennial aquatic plants. In this study, the turion cytochemistry and ultrastructure of Aldrovanda vesiculosa, Utricularia vulgaris and U. stygia were compared with particular emphasis placed on storage substances. These...

journal_title：Protoplasma

authors： Płachno BJ,Adamec L,Kozieradzka-Kiszkurno M,Swiątek P,Kamińska I

更新日期：2014-11-01 00:00:00

abstract：:By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the m...

journal_title：Protoplasma

authors： Grigorjeva VV,Gabarayeva N

更新日期：2018-01-01 00:00:00

abstract：:The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a flight parabola, an acceleration profile is achieved which yields...

journal_title：Protoplasma

authors： Richter PR,Schuster M,Meyer I,Lebert M,Häder DP

更新日期：2006-12-01 00:00:00

abstract：:In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth reg...

journal_title：Protoplasma

authors： Devrnja N,Krstić-Milošević D,Janošević D,Tešević V,Vinterhalter B,Savić J,Ćalić D

更新日期：2020-11-27 00:00:00

abstract：:Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avo...

journal_title：Protoplasma

authors： Guzmán-Deara J,Reyes-De la Cruz H,Beltrán-Peña EM,Castro-Mercado E,García-Pineda E

更新日期：2013-06-01 00:00:00

abstract：:We have analyzed the expression of key genes orchestrating tail regeneration in lizard under normal and scarring conditions after cauterization. At 1-day post-cauterization (1 dpc), the injured blastema contains degenerating epithelial and mesenchymal cells, numerous mast cells, and immune cells. At 3 and 7 dpc, a str...

journal_title：Protoplasma

authors： Degan M,Dalla Valle L,Alibardi L

更新日期：2021-01-01 00:00:00

abstract：:Fruit shape and ripening are major horticultural traits for many fruits and vegetable crops. Changes in fruit shape and ripening are often accomplished by altered cell division or cell expansion patterns. Gibberellic acids (GAs) are essential for tomato fruit development; however, the exact role and the underlying mec...

journal_title：Protoplasma

authors： Chen S,Wang XJ,Tan GF,Zhou WQ,Wang GL

更新日期：2020-05-01 00:00:00

abstract：:Oogenesis in the neotenic, cave dwelling salamander Proteus anguinus anguinus has not been studied yet, and this study provides a detailed description of the early growth of the oocytes. Early previtellogene oocytes ranging from 100 to 600 µm in diameter were examined by light and transmission electron microscopy. The...

journal_title：Protoplasma

authors： Mali LB,Bulog B

更新日期：2010-10-01 00:00:00

abstract：:Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based auxin biosensors to m...

journal_title：Protoplasma

authors： Dubas E,Moravčíková J,Libantová J,Matušíková I,Benková E,Zur I,Krzewska M

更新日期：2014-09-01 00:00:00

abstract：:The purpose of the main research was to investigate the effects of methyl jasmonate (MeJA) (0.05, 0.25, 0.5, and 2.5 mM) on the pollen germination and tube elongation of Pinus nigra. Total pollen germination rate increased after MeJA treatments while the most enhancement was observed at 0.05-mM MeJA. No germination wa...

journal_title：Protoplasma

authors： Çetinbaş-Genç A,Vardar F

更新日期：2020-11-01 00:00:00

abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...

journal_title：Protoplasma

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Tuleja M,Ślesak H,Kapusta P,Marcińska I,Bohdanowicz J

更新日期：2013-02-01 00:00:00

abstract：:The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...

journal_title：Protoplasma

authors： Lütz C

更新日期：2010-08-01 00:00:00

abstract：:The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

journal_title：Protoplasma

authors： Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

更新日期：2003-09-01 00:00:00

abstract：:Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...

journal_title：Protoplasma

authors： Ahanger MA,Tittal M,Mir RA,Agarwal RM

更新日期：2017-09-01 00:00:00

abstract：:Pollen developmental pathway in plants involving synchronized transferal of cellular divisions from meiosis (microsporogenesis) to mitosis (pollen mitosis I/II) eventually offers a unique "meiosis-mitosis shift" at pollen mitosis I. Since the cell type (haploid microspore) and fate of pollen mitosis I differ from typi...

journal_title：Protoplasma

authors： Sharma SK,Yamamoto M,Mukai Y

更新日期：2017-01-01 00:00:00

abstract：:Temperatures above the optimum are sensed as heat stress (HS) by all living organisms and represent one of the major environmental challenges for plants. Plants can cope with HS by activating specific defense mechanisms to minimize damage and ensure cellular functionality. One of the most common effects of HS is the o...

journal_title：Protoplasma

authors： Malerba M,Cerana R

更新日期：2018-07-01 00:00:00

abstract：:The presence of specific protein types in claws and beaks of turtles is poorly known. The present immunological study describes the localization of some of the main corneous beta proteins (CBPs) coded in the epidermal differentiation complex of turtles. Three antibodies here utilized revealed that glycine-, cysteine-,...

journal_title：Protoplasma

authors： Alibardi L

更新日期：2020-07-01 00:00:00