Abstract:
:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium supplemented with auxins and/or cytokinins. The efficiency of endosperm response was highly genotype-dependent and best in two winter cultivars of hexaploid species. The pathways of development and proliferation were very similar among the selected species and cultivars. Histological and scanning electron microscope (SEM) analysis revealed that only the part of the endosperm not touching the medium surface continued growth and development, resulting in swelling. The central part of swollen regions was composed mainly of cells containing many large starch grains. The peripheric parts of developed endosperm consisted of highly vacuolated cells and small cells with dense cytoplasm. SEM showed that cells from the swollen region were covered partially with a membraneous structure. Transmission electron microscope studies of cells from the outer part of the developing region showed features typical for cell activity connected with lipid metabolism.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Tuleja M,Ślesak H,Kapusta P,Marcińska I,Bohdanowicz Jdoi
10.1007/s00709-012-0419-1subject
Has Abstractpub_date
2013-02-01 00:00:00pages
361-9issue
1eissn
0033-183Xissn
1615-6102journal_volume
250pub_type
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