Abstract:
:Capitate glandular trichomes (CGT) of sunflower, Helianthus annuus, synthesize bioactive sesquiterpene lactones (STLs) within a short period of only a few days during trichome development. In the current project, the subcellular localization of H. annuus germacrene A monooxygenase (HaGAO), a key enzyme of the STL biosynthesis in sunflower CGT, was investigated. A polyclonal antibody raised against this enzyme was used for immunolabelling. HaGAO was found in secretory and stalk cells of CGT. This correlated with the appearance of smooth endoplasmic reticulum in both cell types. Stalk cells and secretory cells differed in form, size and types of plastids, but both had structures necessary for secretion. No HaGAO-specific immunoreaction was found in sunflower leaf tissue outside of CGT or in developing CGT before the secretory phase had started. Our results indicated that not only secretory cells but also nearly all cells of the CGT were involved in the biosynthesis of STL and that this process was not linked to the presence or absence of a specific type of plastid.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Amrehn E,Aschenbrenner AK,Heller A,Spring Odoi
10.1007/s00709-015-0823-4subject
Has Abstractpub_date
2016-03-01 00:00:00pages
447-55issue
2eissn
0033-183Xissn
1615-6102pii
10.1007/s00709-015-0823-4journal_volume
253pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-009-0073-4
更新日期:2009-10-01 00:00:00
abstract::A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s007090200021
更新日期:2002-05-01 00:00:00
abstract::In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0033-3
更新日期:2004-06-01 00:00:00
abstract::Salinity and drought are important abiotic stresses limiting plant growth and development. Late embryogenesis abundant (LEA) proteins are a group of proteins associated with tolerance to water-related stress. We previously cloned an LEA gene, SmLEA, from Salvia miltiorrhiza Bunge. Phylogenetic analysis indicated that ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-014-0626-z
更新日期:2014-09-01 00:00:00
abstract::During the past decade, there has been a paradigm shift in our understanding of the roles of intracellular lipid droplets (LDs). New genetic, biochemical and imaging technologies have underpinned these advances, which are revealing much new information about these dynamic organelles. This review takes a comparative ap...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-011-0329-7
更新日期:2012-07-01 00:00:00
abstract::Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01556-3
更新日期:2021-01-01 00:00:00
abstract::Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant d...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0792-7
更新日期:2016-01-01 00:00:00
abstract::Lemna species are reported to accumulate a variety of metals from contaminated/polluted sites. Cadmium is a nonessential element for plant metabolism. In this work, we aimed to investigate physiological responses to low doses of cadmium (up to 100 microM). From exposure to the lowest Cd concentration (1 microM) to the...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-009-0091-2
更新日期:2010-04-01 00:00:00
abstract::The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the fiel...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01398-8
更新日期:2019-11-01 00:00:00
abstract::In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont "Candidatus Sulcia muelleri" (Phylum Bacteroidetes) resides in bacterio...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1033-4
更新日期:2017-05-01 00:00:00
abstract::The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-014-0687-z
更新日期:2015-01-01 00:00:00
abstract::Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0158-0
更新日期:2010-09-01 00:00:00
abstract::Temperatures above the optimum are sensed as heat stress (HS) by all living organisms and represent one of the major environmental challenges for plants. Plants can cope with HS by activating specific defense mechanisms to minimize damage and ensure cellular functionality. One of the most common effects of HS is the o...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1200-2
更新日期:2018-07-01 00:00:00
abstract::In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by up...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0756-y
更新日期:2015-09-01 00:00:00
abstract::The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0110-3
更新日期:2010-05-01 00:00:00
abstract::Oogenesis in the neotenic, cave dwelling salamander Proteus anguinus anguinus has not been studied yet, and this study provides a detailed description of the early growth of the oocytes. Early previtellogene oocytes ranging from 100 to 600 µm in diameter were examined by light and transmission electron microscopy. The...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0117-9
更新日期:2010-10-01 00:00:00
abstract::The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0161-5
更新日期:2010-08-01 00:00:00
abstract::While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-002-0040-9
更新日期:2003-03-01 00:00:00
abstract::Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First, the volume fraction...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01304490
更新日期:2000-01-01 00:00:00
abstract::The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01404042
更新日期:1993-01-01 00:00:00
abstract::The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions i...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-005-0123-5
更新日期:2005-12-01 00:00:00
abstract::The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0145-5
更新日期:2010-11-01 00:00:00
abstract::Formation of intra-molecular disulfides and concomitant oxidative protein folding is essential for stability and catalytic function of many soluble and membrane-bound proteins in the endomembrane system, the mitochondrial inter-membrane space and the thylakoid lumen. Disulfide generation from free cysteines in nascent...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-012-0463-x
更新日期:2013-08-01 00:00:00
abstract::Approximately 20,000 mammalian genes are estimated to encode between 250 thousand and 1 million different proteins. This enormous diversity of the mammalian proteome is caused by the ability of a single-gene locus to encode multiple protein isoforms. Protein isoforms encoded by one gene locus can be functionally disti...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-016-1071-y
更新日期:2017-05-01 00:00:00
abstract::Interactive effects of two heavy metal pollutants Cd and Pb in the growth medium were examined on their uptake, production of reactive oxygen species (ROS), induction of oxidative stress and antioxidative defence responses in Indica rice (Oryza sativa L.) seedlings. When rice seedlings in sand culture were exposed to ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-014-0614-3
更新日期:2014-09-01 00:00:00
abstract::Proper mitochondrial morphogenesis is crucial for successful development of motile sperm. It was known that recessive Drosophila melanogaster mutation emm caused anomalies in the formation of a mitochondrial derivative--nebenkern and led to male sterility. Here we identified primary mutation effect and showed that emm...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-012-0434-2
更新日期:2013-04-01 00:00:00
abstract::The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1221-5
更新日期:2018-07-01 00:00:00
abstract::Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0896-0
更新日期:2016-11-01 00:00:00
abstract::Phenolics are involved in many of plants' biological functions. In particular, they play important roles in determining the quality of grape berries and the wine made from them, and can also act as antioxidants with beneficial effects for human health. Several enzymes are involved in the synthesis of phenolic compound...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1278-1
更新日期:2019-01-01 00:00:00
abstract::The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0875-5
更新日期:2016-07-01 00:00:00