Responses of Lemna trisulca L. (Duckweed) exposed to low doses of cadmium: thiols, metal binding complexes, and photosynthetic pigments as sensitive biomarkers of ecotoxicity.

Abstract:

:Lemna species are reported to accumulate a variety of metals from contaminated/polluted sites. Cadmium is a nonessential element for plant metabolism. In this work, we aimed to investigate physiological responses to low doses of cadmium (up to 100 microM). From exposure to the lowest Cd concentration (1 microM) to the highest (100 microM), photosynthetic pigments (Chl a, b, carotenoids) and the ratios of Chl a/b, Chl (a + b)/carotenoids decreased as a function of the Cd dose. The content of soluble proteins decreased in a dose-dependent manner, while total soluble thiols drastically increased. In Cd-treated fronds, the dose-dependent accumulation of a polypeptide with an apparent molecular weight of 24 kDa, as well as the appearance of two smaller polypeptides with molecular weights <6.5 kDa, was observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. Our results show that in Lemna trisulca, different adaptative mechanisms may be involved in counterbalancing low and high doses of a particular toxicant (cadmium). This feature makes this plant potentially useful material in biomonitoring and phytotoxicity testing.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Malec P,Maleva MG,Prasad MN,Strzałka K

doi

10.1007/s00709-009-0091-2

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

69-74

issue

1-4

eissn

0033-183X

issn

1615-6102

journal_volume

240

pub_type

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