Visualization by atomic force microscopy and FISH of the 45S rDNA gaps in mitotic chromosomes of Lolium perenne.

abstract：:Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is characterized by multistage formation. The presence of ductular reaction, cytokeratin 7 positivity (PCK7), and increased levels of gamma glutamyltransferase (γGT) has been observed during liver carcinogenesis and contribute to tum...

journal_title：Protoplasma

authors： Moreira AJ,Rodrigues GR,Bona S,Fratta LX,Weber GR,Picada JN,Dos Santos JL,Cerski CT,Marroni CA,Marroni NP

更新日期：2017-03-01 00:00:00

abstract：:It is well established that cytoplasmic microtubules are depolymerized during nuclear division and reassembled as mitotic microtubules. Mounting evidence showing that cytoplasmic microtubules were also involved in apical growth of fungal hyphae posed the question of whether apical growth became disrupted during nuclea...

journal_title：Protoplasma

authors： Riquelme M,Fischer R,Bartnicki-García S

更新日期：2003-01-01 00:00:00

abstract：:Salt stress in the rice field is one of the most common abiotic stresses, reducing crop productivity, especially at reproductive stage, which is very sensitive to salt stress. The aim of this investigation was to study mRNA-related Na+ uptake/translocation and Na+ enrichment in the cellular level, leading to physiolog...

journal_title：Protoplasma

authors： Theerawitaya C,Samphumphuang T,Tisarum R,Siangliw M,Cha-Um S,Takabe T,Toojinda T

更新日期：2020-11-01 00:00:00

abstract：:The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...

journal_title：Protoplasma

authors： Zheng Y,Wang Z

更新日期：2015-01-01 00:00:00

abstract：:Some species of Spirogyra form rosette-shaped or rod-shaped rhizoids in the terminal cell of the filaments. In the present study, we analyzed an involvement of microtubules (MTs) in rhizoid differentiation. Before rhizoid differentiation, cortical MTs were arranged transversely to the long axis of cylindrical cells, r...

journal_title：Protoplasma

authors： Yoshida K,Inoue N,Sonobe S,Shimmen T

更新日期：2003-06-01 00:00:00

abstract：:Despite their great economic importance, relatively little is known about bamboo sexual reproduction because they usually spread through rhizomes and have long intervals between flowering periods. Bambusa tuldoides is no exception; the intervals between flowering periods are about 23 years and often do not result in s...

journal_title：Protoplasma

authors： de Souza PF,Dos Santos CMR,Ree J,Guerra MP,Pescador R

更新日期：2020-05-01 00:00:00

abstract：:Interactive effects of two heavy metal pollutants Cd and Pb in the growth medium were examined on their uptake, production of reactive oxygen species (ROS), induction of oxidative stress and antioxidative defence responses in Indica rice (Oryza sativa L.) seedlings. When rice seedlings in sand culture were exposed to ...

journal_title：Protoplasma

authors： Srivastava RK,Pandey P,Rajpoot R,Rani A,Dubey RS

更新日期：2014-09-01 00:00:00

abstract：:Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when ...

journal_title：Protoplasma

authors： Choudhury RR,Basak S,Ramesh AM,Rangan L

更新日期：2014-05-01 00:00:00

abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...

journal_title：Protoplasma

authors： Hensel W

更新日期：1985-01-01 00:00:00

abstract：:Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based auxin biosensors to m...

journal_title：Protoplasma

authors： Dubas E,Moravčíková J,Libantová J,Matušíková I,Benková E,Zur I,Krzewska M

更新日期：2014-09-01 00:00:00

abstract：:Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...

journal_title：Protoplasma

authors： Oleszczuk S,Sowa S,Zimny J

更新日期：2006-08-01 00:00:00

abstract：:Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the ...

journal_title：Protoplasma

authors： Wachsmuth M

更新日期：2014-03-01 00:00:00

abstract：:Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...

journal_title：Protoplasma

authors： Mursalimov S,Deineko E

更新日期：2017-05-01 00:00:00

abstract：:In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...

journal_title：Protoplasma

authors： Kanu SA,Dakora FD

更新日期：2017-01-01 00:00:00

abstract：:The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...

journal_title：Protoplasma

authors： Liu L,Hua BZ

更新日期：2018-07-01 00:00:00

abstract：:Capitate glandular trichomes (CGT) of sunflower, Helianthus annuus, synthesize bioactive sesquiterpene lactones (STLs) within a short period of only a few days during trichome development. In the current project, the subcellular localization of H. annuus germacrene A monooxygenase (HaGAO), a key enzyme of the STL bios...

journal_title：Protoplasma

authors： Amrehn E,Aschenbrenner AK,Heller A,Spring O

更新日期：2016-03-01 00:00:00

abstract：:Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-pa...

journal_title：Protoplasma

authors： Giannoutsou EP,Apostolakos P,Galatis B

更新日期：2011-04-01 00:00:00

abstract：:Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

journal_title：Protoplasma

authors： Malerba M,Cerana R,Crosti P

更新日期：2004-10-01 00:00:00

abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...

journal_title：Protoplasma

authors： Amnuaykanjanasin A,Epstein L

更新日期：2006-05-01 00:00:00

abstract：:Phenolics are involved in many of plants' biological functions. In particular, they play important roles in determining the quality of grape berries and the wine made from them, and can also act as antioxidants with beneficial effects for human health. Several enzymes are involved in the synthesis of phenolic compound...

journal_title：Protoplasma

authors： Molero de Ávila ME,Alarcón MV,Uriarte D,Mancha LA,Moreno D,Francisco-Morcillo J

更新日期：2019-01-01 00:00:00

abstract：:The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

journal_title：Protoplasma

authors： Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

更新日期：2003-09-01 00:00:00

abstract：:The differentiation of the corneous layers of lizard epidermis has been analyzed by ultrastructural immunocytochemistry using specific antibodies against alpha-keratins and keratin associated beta-proteins (KAbetaPs, formerly indicated as beta-keratins). Both beta-cells and alpha-cells of the corneous layer derive fro...

journal_title：Protoplasma

authors： Alibardi L

更新日期：2014-07-01 00:00:00

abstract：:Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...

journal_title：Protoplasma

authors： Gügel IL,Soll J

更新日期：2017-09-01 00:00:00

abstract：:Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...

journal_title：Protoplasma

authors： Singla P,Bhardwaj RD,Kaur S,Kaur J

更新日期：2020-01-01 00:00:00

abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

journal_title：Protoplasma

authors： Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

更新日期：2017-09-01 00:00:00

abstract：:In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by up...

journal_title：Protoplasma

authors： Shan C,Zhou Y,Liu M

更新日期：2015-09-01 00:00:00

abstract：:In order to gain more insight into the involvement of mitochondrial complex III in the Cd-induced stress, we studied the effect of complex III inhibitors, antimycin A (AA), and myxothiazol (MYXO), on the Cd-induced ROS and NO generation in the barley root tip. Short-term exposure of barley roots to either MYXO or AA p...

journal_title：Protoplasma

authors： Zelinová V,Demecsová L,Tamás L

更新日期：2019-09-01 00:00:00

abstract：:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...

journal_title：Protoplasma

authors： Farooq S,Fijen C,Hohlbein J

更新日期：2014-03-01 00:00:00

abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...

journal_title：Protoplasma

authors： Talbot MJ,Offler CE,McCurdy DW

更新日期：2002-05-01 00:00:00

abstract：:Palaeontologically, eubacteria are > 3× older than neomura (eukaryotes, archaebacteria). Cell biology contrasts ancestral eubacterial murein peptidoglycan walls and derived neomuran N-linked glycoprotein coats/walls. Misinterpreting long stems connecting clade neomura to eubacteria on ribosomal sequence trees (plus mi...

journal_title：Protoplasma

authors： Cavalier-Smith T,Chao EE

更新日期：2020-05-01 00:00:00