Abstract：
:Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic microspore development could be induced from treated and nontreated microspores, abiotic stress was essential for embryo formation and plant regeneration. The type of stress treatment applied affected the numbers and the ratios of albino and green plants regenerated, as well as their fertility. The highest number of green plants was obtained after the treatment of anthers in 0.3 M mannitol at 32 degrees C for 24 h before microspore culture.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Oleszczuk S,Sowa S,Zimny Jdoi
10.1007/s00709-006-0179-xsubject
Has Abstractpub_date
2006-08-01 00:00:00pages
95-100issue
1-3eissn
0033-183Xissn
1615-6102journal_volume
228pub_type
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