Extra thin alginate film: an efficient technique for protoplast culture.

abstract：:The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...

journal_title：Protoplasma

authors： Pradhan M,Sahoo RK,Pradhan C,Tuteja N,Mohanty S

更新日期：2017-11-01 00:00:00

abstract：:Vicia barbazitae, a taxon belonging to section Vicia of subgenus Vicia, was recovered and analysed by cytological, karyological and molecular methods with the aim of both proposing a general characterisation of this species and studying the relationships among the species of section Vicia . Phy...

journal_title：Protoplasma

authors： Ruffini Castiglione M,Frediani M,Gelati MT,Venora G,Giorgetti L,Caputo P,Cremonini R

更新日期：2012-07-01 00:00:00

abstract：:The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis. As very little is known on the molecular level about mycorrhi...

journal_title：Protoplasma

authors： Menotta M,Amicucci A,Basili G,Rivero F,Polidori E,Sisti D,Stocchi V

更新日期：2007-01-01 00:00:00

abstract：:The osmotic potential and effects of plasmolysis were investigated in two different Klebsormidium strains from alpine habitats by incubation in 300-2,000 (3,000) mM sorbitol. Several members of this genus were previously found to tolerate desiccation in the vegetative state yet information was lacking on the osmotic p...

journal_title：Protoplasma

authors： Kaplan F,Lewis LA,Wastian J,Holzinger A

更新日期：2012-07-01 00:00:00

abstract：:Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant d...

journal_title：Protoplasma

authors： Ranjit SL,Manish P,Penna S

更新日期：2016-01-01 00:00:00

abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

journal_title：Protoplasma

authors： Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

更新日期：2017-09-01 00:00:00

abstract：:Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...

journal_title：Protoplasma

authors： Eremin A,Bulychev AA,Kluge C,Harbinson J,Foissner I

更新日期：2019-11-01 00:00:00

abstract：:The extent to which the actomyosin motor responsible for cytoplasmic streaming contributes to the translational diffusion of water in Elodea canadensis cells was studied by a nuclear magnetic resonance (NMR) spin-echo technique. The relative contribution of the actomyosin motor was determined from the corresponding ap...

journal_title：Protoplasma

authors： Vorob'ev VN,Anisimov AV,Dautova NR

更新日期：2004-12-01 00:00:00

abstract：:Along the past recent years, two-photon excitation (TPE) microscopy has moved from the realms of technical curiosity to be a standard application in many advanced cell biology laboratories. The growing body of literature covered in this review points out the obvious advantages of TPE over any other imaging method base...

journal_title：Protoplasma

authors： Feijó JA,Moreno N

更新日期：2004-03-01 00:00:00

abstract：:Podophyllum hexandrum Royle is known for its vast medicinal properties, particularly anticancer. It contains higher amount of podophyllotoxin (4.3 %), compared to Podophyllum peltatum (0.025 %) and other plant species; as a result, it has been used worldwide in the preparation of various drugs including anticancer, an...

journal_title：Protoplasma

authors： Kumar P,Pal T,Sharma N,Kumar V,Sood H,Chauhan RS

更新日期：2015-09-01 00:00:00

abstract：:The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal...

journal_title：Protoplasma

authors： Souguir D,Ferjani E,Ledoigt G,Goupil P

更新日期：2008-11-01 00:00:00

abstract：:Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...

journal_title：Protoplasma

authors： Imani R,Pazoki M,Zupančič D,Kreft ME,Kralj-Iglič V,Veranič P,Iglič A

更新日期：2016-11-01 00:00:00

abstract：:The original version of this article unfortunately contains a mistake. ...

journal_title：Protoplasma

authors： Asada T

更新日期：2019-05-01 00:00:00

abstract：:Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based auxin biosensors to m...

journal_title：Protoplasma

authors： Dubas E,Moravčíková J,Libantová J,Matušíková I,Benková E,Zur I,Krzewska M

更新日期：2014-09-01 00:00:00

abstract：:Palaeontologically, eubacteria are > 3× older than neomura (eukaryotes, archaebacteria). Cell biology contrasts ancestral eubacterial murein peptidoglycan walls and derived neomuran N-linked glycoprotein coats/walls. Misinterpreting long stems connecting clade neomura to eubacteria on ribosomal sequence trees (plus mi...

journal_title：Protoplasma

authors： Cavalier-Smith T,Chao EE

更新日期：2020-05-01 00:00:00

abstract：:Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...

journal_title：Protoplasma

authors： Singla P,Bhardwaj RD,Kaur S,Kaur J

更新日期：2020-01-01 00:00:00

abstract：:To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, ...

journal_title：Protoplasma

authors： Sun X,Lian H,Liu X,Zhou S,Liu S

更新日期：2017-05-01 00:00:00

abstract：:According to the Cholodny-Went hypothesis, gravitropic differential growth is brought about by the redistribution of auxin (indolyl-3-acetic acid, IAA). We reinvestigated the relevance of different auxins and studied the role of ethylene in hypocotyls of sunflower and shoots and roots of rye and maize seedlings. Incub...

journal_title：Protoplasma

authors： Edelmann HG,Roth U

更新日期：2006-12-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...

journal_title：Protoplasma

authors： Langer T,Fasold H

更新日期：2001-01-01 00:00:00

abstract：:In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by up...

journal_title：Protoplasma

authors： Shan C,Zhou Y,Liu M

更新日期：2015-09-01 00:00:00

abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

journal_title：Protoplasma

authors： Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

更新日期：2006-08-01 00:00:00

abstract：:In this study, the filamentous green alga Zygogonium ericetorum (Zygnematales, Chlorophyta), collected at its natural habitat in the high alps, was investigated by light, scanning, and transmission electron microscopy. The field samples were separated into a moist fraction when wetted by splattering water of a nearby ...

journal_title：Protoplasma

authors： Holzinger A,Tschaikner A,Remias D

更新日期：2010-07-01 00:00:00

abstract：:Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

journal_title：Protoplasma

authors： Malerba M,Cerana R,Crosti P

更新日期：2004-10-01 00:00:00

abstract：:The distribution and development of secretory cells and crystal cells in young shoot apexes of water hyacinth were investigated through morphological and cytological analysis. The density of secretory cells and crystal cells were high in parenchyma tissues around the vascular bundles of shoot apexes. Three development...

journal_title：Protoplasma

authors： Xu GX,Tan C,Wei XJ,Gao XY,Zheng HQ

更新日期：2011-04-01 00:00:00

abstract：:Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...

journal_title：Protoplasma

authors： Alla MM,Hassan NM

更新日期：2012-10-01 00:00:00

abstract：:Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...

journal_title：Protoplasma

authors： Sobol M,Gonzalez-Camacho F,Rodríguez-Vilariño V,Kordyum E,Medina FJ

更新日期：2006-09-01 00:00:00

abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...

journal_title：Protoplasma

authors： Yadav R,Mehrotra M,Singh AK,Niranjan A,Singh R,Sanyal I,Lehri A,Pande V,Amla DV

更新日期：2017-01-01 00:00:00

abstract：:A convenient experimental system was established to test how cells derived from higher-plant internal tissues respond to mechanical stimulation. Short-term culture of tobacco ovules in vitro led to the generation of bar-shaped cells from the parenchyma tissue of the ovule funicle. These cells are still connected to th...

journal_title：Protoplasma

authors： Qu LH,Sun MX

更新日期：2008-01-01 00:00:00

abstract：:Vacuoles are ubiquitous organelles in the fungal and plant kingdoms. They serve a variety of functions and are important for cell homeostasis. A constant turnover of proteins and membranes makes vacuoles dynamic organelles. Various transport pathways share the vacuole as their joint destination. The trafficking pathwa...

journal_title：Protoplasma

authors： Götte M,Lazar T

更新日期：1999-01-01 00:00:00

abstract：:With the goal of evaluating the factors that may be influencing the reproductive success of an endangered, terrestrial orchid Cypripedium japonicum Thunb., the anatomical and morphological characteristics of seed development were assessed, as were the levels of endogenous hormones to investigate the possible relations...

journal_title：Protoplasma

authors： Yan X,Tian M,Liu F,Wang C,Zhang Y

更新日期：2017-11-01 00:00:00