Molecular diffusion and binding analyzed with FRAP.

Abstract:

:Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the last years, this has become a very powerful tool to study dynamic processes in living cells and tissue, and it is expected to experience further increasing demand because quantitative information on biological systems becomes more and more important. This review introduces the FRAP methodology, including some theoretical background, describes challenges and pitfalls, and presents some recent advanced applications.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Wachsmuth M

doi

10.1007/s00709-013-0604-x

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

373-82

issue

2

eissn

0033-183X

issn

1615-6102

journal_volume

251

pub_type

杂志文章,评审
  • Glucose-induced response on photosynthetic efficiency, ROS homeostasis, and antioxidative defense system in maintaining carbohydrate and ion metabolism in Indian mustard (Brassica juncea L.) under salt-mediated oxidative stress.

    abstract::In plants, glucose (Glc) acts as a crucial signaling molecule in mediating metabolism, growth, stress tolerance mechanism, etc. However, little is known about Glc supplementation in salinity tolerance. This experiment was designed to study the ameliorative effect of Glc in mustard under salt stress. The seeds were soa...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01600-2

    authors: Sami F,Siddiqui H,Alam P,Hayat S

    更新日期:2021-01-03 00:00:00

  • Contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (Arachis hypogaea L.).

    abstract::The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1112-1

    authors: Pradhan M,Sahoo RK,Pradhan C,Tuteja N,Mohanty S

    更新日期:2017-11-01 00:00:00

  • Chloroplast differentiation in the growing leaves of Arabidopsis thaliana.

    abstract::Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1057-9

    authors: Gügel IL,Soll J

    更新日期:2017-09-01 00:00:00

  • Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.).

    abstract::Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant d...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0792-7

    authors: Ranjit SL,Manish P,Penna S

    更新日期:2016-01-01 00:00:00

  • In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents.

    abstract::In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth reg...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01588-9

    authors: Devrnja N,Krstić-Milošević D,Janošević D,Tešević V,Vinterhalter B,Savić J,Ćalić D

    更新日期:2020-11-27 00:00:00

  • Morpho-histological, histochemical, and molecular evidences related to cellular reprogramming during somatic embryogenesis of the model grass Brachypodium distachyon.

    abstract::The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1089-9

    authors: Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

    更新日期:2017-09-01 00:00:00

  • Effects of high temperature on the ultrastructure and microtubule organization of interphase and dividing cells of the seagrass Cymodocea nodosa.

    abstract::Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0809-2

    authors: Koutalianou M,Orfanidis S,Katsaros C

    更新日期:2016-03-01 00:00:00

  • Is 2,3-butanedione monoxime an effective inhibitor of myosin-based activities in plant cells?

    abstract::The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01415707

    authors: McCurdy DW

    更新日期:1999-01-01 00:00:00

  • Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes.

    abstract::We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-007-0265-8

    authors: Forer A,Spurck T,Pickett-Heaps JD

    更新日期:2007-01-01 00:00:00

  • A dual switch in phloem unloading during ovule development in Arabidopsis.

    abstract::Developing flowers are important sinks in Arabidopsis thaliana. Their energy demand is covered by assimilates which are synthesized in source leaves and transported via the vasculature. Assimilates are unloaded either symplastically through plasmodesmata or apoplastically by specific transport proteins. Here we studie...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-010-0223-8

    authors: Werner D,Gerlitz N,Stadler R

    更新日期:2011-01-01 00:00:00

  • Lichen secondary metabolites affect growth of Physcomitrella patens by allelopathy.

    abstract::Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1022-7

    authors: Goga M,Antreich SJ,Bačkor M,Weckwerth W,Lang I

    更新日期:2017-05-01 00:00:00

  • Unusual developmental morphology and anatomy of vegetative organs in Utricularia dichotoma-leaf, shoot and root dynamics.

    abstract::The terrestrial carnivorous species Utricularia dichotoma is known for a great phenotypic plasticity and unusual vegetative organs. Our investigation on 22 sources/populations revealed that after initiation of a leaf and two bladders on a stolon, a bud was formed in the proximal axil of the leaf, developing into a ros...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01443-6

    authors: Reut MS,Płachno BJ

    更新日期:2020-03-01 00:00:00

  • The garlic NF-YC gene, AsNF-YC8, positively regulates non-ionic hyperosmotic stress tolerance in tobacco.

    abstract::To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1026-3

    authors: Sun X,Lian H,Liu X,Zhou S,Liu S

    更新日期:2017-05-01 00:00:00

  • Development of the cotyledon cells during olive (Olea europaea L.) in vitro seed germination and seedling growth.

    abstract::The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-010-0242-5

    authors: Zienkiewicz A,Jiménez-López JC,Zienkiewicz K,de Dios Alché J,Rodríguez-García MI

    更新日期:2011-10-01 00:00:00

  • Ultrastructure of the larval Malpighian tubules in Terrobittacus implicatus (Mecoptera: Bittacidae).

    abstract::The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-018-1221-5

    authors: Liu L,Hua BZ

    更新日期:2018-07-01 00:00:00

  • Inhibition of lindane-induced toxicity using alpha-lipoic acid and vitamin E in the brain of Mus musculus.

    abstract::In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-010-0121-0

    authors: Bist R,Misra S,Bhatt DK

    更新日期:2010-06-01 00:00:00

  • Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins.

    abstract::We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-005-0092-8

    authors: Tien CJ,Sigee DC,White KN

    更新日期:2005-10-01 00:00:00

  • Covisualization by computational optical-sectioning microscopy of integrin and associated proteins at the cell membrane of living onion protoplasts.

    abstract::Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01882029

    authors: Gens JS,Reuzeau C,Doolittle KW,McNally JG,Pickard BG

    更新日期:1996-01-01 00:00:00

  • Decondensation of chromosomal 45S rDNA sites in Lolium and Festuca genotypes does not result in karyotype instability.

    abstract::Fragile sites (FSs) in plants have been described for species like Lolium and other grasses. Whereas in humans FSs were shown to be involved in genome instabilities; the consequences of FSs expression in plants are not known yet. To evaluate whether FSs cause karyotype instabilities, we assessed the frequency of micro...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-0942-6

    authors: Rocha LC,Jankowska M,Fuchs J,Mittelmann A,Techio VH,Houben A

    更新日期:2017-01-01 00:00:00

  • Live-cell imaging of tubulin in the filamentous fungus Magnaporthe grisea treated with anti-microtubule and anti-microfilament agents.

    abstract::Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-004-0081-3

    authors: Czymmek KJ,Bourett TM,Shao Y,DeZwaan TM,Sweigard JA,Howard RJ

    更新日期:2005-04-01 00:00:00

  • Root-to-shoot signalling: apoplastic alkalinization, a general stress response and defence factor in barley (Hordeum vulgare).

    abstract::We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-005-0131-5

    authors: Felle HH,Herrmann A,Hückelhoven R,Kogel KH

    更新日期:2005-12-01 00:00:00

  • Anatomy and lignin deposition of stone cell in Camellia oleifera shell during the young stage.

    abstract::As the by-products of edible oil production with rich lignin, the reserves of Camellia oleifera shell were abundant and had a great economic value. Lignin was the most important limiting factor during the conversion of plant biomass to pulp or biofuels, which mainly deposited in the stone cells of C. oleifera shells. ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01568-z

    authors: Wang Q,Hu J,Yang T,Chang S

    更新日期:2020-10-26 00:00:00

  • Ductular reaction, cytokeratin 7 positivity, and gamma-glutamyl transferase in multistage hepatocarcinogenesis in rats.

    abstract::Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is characterized by multistage formation. The presence of ductular reaction, cytokeratin 7 positivity (PCK7), and increased levels of gamma glutamyltransferase (γGT) has been observed during liver carcinogenesis and contribute to tum...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1000-0

    authors: Moreira AJ,Rodrigues GR,Bona S,Fratta LX,Weber GR,Picada JN,Dos Santos JL,Cerski CT,Marroni CA,Marroni NP

    更新日期:2017-03-01 00:00:00

  • Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples.

    abstract::The plant root tip represents a fascinating model system for studying changes in Golgi stack architecture associated with the developmental progression of meristematic cells to gravity sensing columella cells, and finally to "young" and "old", polysaccharide-slime secreting peripheral cells. To this end we have used ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01322640

    authors: Staehelin LA,Giddings TH Jr,Kiss JZ,Sack FD

    更新日期:1990-01-01 00:00:00

  • Identification of a novel plant-specific kinesin-like protein that is highly expressed in interphase tobacco BY-2 cells.

    abstract::Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01280307

    authors: Matsui K,Collings D,Asada T

    更新日期:2001-01-01 00:00:00

  • Distribution and function of epistomatal mucilage plugs.

    abstract::Investigation of 67 gymnosperm and angiosperm species belonging to 25 orders shows that epistomatal mucilage plugs are a widespread phenomenon. Measurements of the leaf water status by using the leaf patch clamp pressure technique suggest that the mucilage plugs are involved in moisture uptake and buffering leaf cells...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-008-0029-0

    authors: Westhoff M,Zimmermann D,Zimmermann G,Gessner P,Wegner LH,Bentrup FW,Zimmermann U

    更新日期:2009-03-01 00:00:00

  • Gene expression in regenerating and scarring tails of lizard evidences three main key genes (wnt2b, egfl6, and arhgap28) activated during the regulated process of tail regeneration.

    abstract::We have analyzed the expression of key genes orchestrating tail regeneration in lizard under normal and scarring conditions after cauterization. At 1-day post-cauterization (1 dpc), the injured blastema contains degenerating epithelial and mesenchymal cells, numerous mast cells, and immune cells. At 3 and 7 dpc, a str...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-020-01545-6

    authors: Degan M,Dalla Valle L,Alibardi L

    更新日期:2021-01-01 00:00:00

  • Overexpression of SmLEA enhances salt and drought tolerance in Escherichia coli and Salvia miltiorrhiza.

    abstract::Salinity and drought are important abiotic stresses limiting plant growth and development. Late embryogenesis abundant (LEA) proteins are a group of proteins associated with tolerance to water-related stress. We previously cloned an LEA gene, SmLEA, from Salvia miltiorrhiza Bunge. Phylogenetic analysis indicated that ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-014-0626-z

    authors: Wu Y,Liu C,Kuang J,Ge Q,Zhang Y,Wang Z

    更新日期:2014-09-01 00:00:00

  • Stripe rust induced defence mechanisms in the leaves of contrasting barley genotypes (Hordeum vulgare L.) at the seedling stage.

    abstract::Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01428-5

    authors: Singla P,Bhardwaj RD,Kaur S,Kaur J

    更新日期:2020-01-01 00:00:00

  • Comparison between the effects of fusicoccin, Tunicamycin, and Brefeldin A on programmed cell death of cultured sycamore (Acer pseudoplatanus L.) cells.

    abstract::Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-004-0053-7

    authors: Malerba M,Cerana R,Crosti P

    更新日期:2004-10-01 00:00:00