Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes.

abstract：:We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...

journal_title：Protoplasma

authors： Forer A,Pickett-Heaps JD,Spurck T

更新日期：2008-01-01 00:00:00

abstract：:Flagellar development in the plurilocular zoidangia of sporophytes of the brown alga Ectocarpus siliculosus was analyzed in detail using transmission electron microscopy and electron tomography. A series of cell divisions in the plurilocular zoidangia produced the spore-mother cells. In these cells, the centrioles dif...

journal_title：Protoplasma

authors： Fu G,Nagasato C,Ito T,Müller DG,Motomura T

更新日期：2013-02-01 00:00:00

abstract：:As the by-products of edible oil production with rich lignin, the reserves of Camellia oleifera shell were abundant and had a great economic value. Lignin was the most important limiting factor during the conversion of plant biomass to pulp or biofuels, which mainly deposited in the stone cells of C. oleifera shells. ...

journal_title：Protoplasma

authors： Wang Q,Hu J,Yang T,Chang S

更新日期：2020-10-26 00:00:00

abstract：:Ripening which leads to fruit senescence is an inimitable process characterized by vivid changes in color, texture, flavor, and aroma of the fleshy fruits. Our understanding of the mechanisms underlying the regulation of fruit ripening and senescence is far from complete. Molecular and biochemical studies on tomato (S...

journal_title：Protoplasma

authors： Kumar V,Irfan M,Ghosh S,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-03-01 00:00:00

abstract：:The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...

journal_title：Protoplasma

authors： Braun M,Sievers A

更新日期：1993-01-01 00:00:00

abstract：:DNA and machinery for gene expression have been discovered in chloroplasts during the 1960s. It was soon evident that the chloroplast genome is relatively small, that most genes for chloroplast-localized proteins reside in the nucleus and that chloroplast membranes, ribosomes, and protein complexes are composed of pro...

journal_title：Protoplasma

authors： Börner T

更新日期：2017-09-01 00:00:00

abstract：:In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...

journal_title：Protoplasma

authors： Kanu SA,Dakora FD

更新日期：2017-01-01 00:00:00

abstract：:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a ma...

journal_title：Protoplasma

authors： Feki K,Tounsi S,Masmoudi K,Brini F

更新日期：2017-07-01 00:00:00

abstract：:Pollen development and germination were adversely affected by the presence of mercury, whereas low-concentrations stimulated the whole procedure. Mercury caused morphological anomalies during the tube growth, characterized by irregularly increasing diameters and swelling tips. The main effect was the anomalous cell wa...

journal_title：Protoplasma

authors： Sawidis T,Baycu G,Cevahir-Öz G,Weryszko-Chmielewska E

更新日期：2018-05-01 00:00:00

abstract：:In the vascular plant Arabidopsis thaliana, synthesis of cysteine and its precursors O-acetylserine and sulfide is distributed between the cytosol, chloroplasts, and mitochondria. This compartmentation contributes to regulation of cysteine synthesis. In contrast to Arabidopsis, cysteine synthesis is exclusively restri...

journal_title：Protoplasma

authors： Birke H,Müller SJ,Rother M,Zimmer AD,Hoernstein SN,Wesenberg D,Wirtz M,Krauss GJ,Reski R,Hell R

更新日期：2012-06-01 00:00:00

abstract：:Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...

journal_title：Protoplasma

authors： Souza LD,Simioni C,Bouzon ZL,Schneider RC,Gressler P,Miotto MC,Rossi MJ,Rörig LR

更新日期：2017-05-01 00:00:00

abstract：:Pollen developmental pathway in plants involving synchronized transferal of cellular divisions from meiosis (microsporogenesis) to mitosis (pollen mitosis I/II) eventually offers a unique "meiosis-mitosis shift" at pollen mitosis I. Since the cell type (haploid microspore) and fate of pollen mitosis I differ from typi...

journal_title：Protoplasma

authors： Sharma SK,Yamamoto M,Mukai Y

更新日期：2017-01-01 00:00:00

abstract：:This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...

journal_title：Protoplasma

authors： Giannoutsou E,Sotiriou P,Nikolakopoulou TL,Galatis B,Apostolakos P

更新日期：2020-01-01 00:00:00

abstract：:Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...

journal_title：Protoplasma

authors： Singla P,Bhardwaj RD,Kaur S,Kaur J

更新日期：2020-01-01 00:00:00

abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...

journal_title：Protoplasma

authors： Yadav R,Mehrotra M,Singh AK,Niranjan A,Singh R,Sanyal I,Lehri A,Pande V,Amla DV

更新日期：2017-01-01 00:00:00

abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...

journal_title：Protoplasma

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Tuleja M,Ślesak H,Kapusta P,Marcińska I,Bohdanowicz J

更新日期：2013-02-01 00:00:00

abstract：:Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...

journal_title：Protoplasma

authors： Czymmek KJ,Bourett TM,Shao Y,DeZwaan TM,Sweigard JA,Howard RJ

更新日期：2005-04-01 00:00:00

abstract：:The larvae of Bittacidae, a cosmopolitan family in Mecoptera, have an interesting habit of spraying the body surface with soil through the anus after hatching, and each molts. The fine structure of Malpighian tubules, however, remains largely unknown in the larvae of Bittacidae to date. Here, we studied the ultrastruc...

journal_title：Protoplasma

authors： Liu L,Hua BZ

更新日期：2018-07-01 00:00:00

abstract：:Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...

journal_title：Protoplasma

authors： Alla MM,Hassan NM

更新日期：2012-10-01 00:00:00

abstract：:The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...

journal_title：Protoplasma

authors： Zienkiewicz A,Jiménez-López JC,Zienkiewicz K,de Dios Alché J,Rodríguez-García MI

更新日期：2011-10-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...

journal_title：Protoplasma

authors： Millaku A,Leser V,Drobne D,Godec M,Torkar M,Jenko M,Milani M,Tatti F

更新日期：2010-05-01 00:00:00

abstract：:HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemi...

journal_title：Protoplasma

authors： Tuteja N,Tarique M,Trivedi DK,Sahoo RK,Tuteja R

更新日期：2015-11-01 00:00:00

abstract：:Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...

journal_title：Protoplasma

authors： Li J,Xu Y,Chong K

更新日期：2012-06-01 00:00:00

abstract：:Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...

journal_title：Protoplasma

authors： Wang H,Wu Y,Yang X,Guo X,Cao X

更新日期：2017-03-01 00:00:00

abstract：:The LIM domain is an evolutionary conserved double-zinc finger motif found in a variety of proteins exhibiting diverse biological roles. LIM domains have been observed to act as modular protein-binding interfaces mediating protein-protein interactions in the cytoplasm and the nucleus. Interaction of LIM domains with s...

journal_title：Protoplasma

authors： Khurana T,Khurana B,Noegel AA

更新日期：2002-02-01 00:00:00

abstract：:By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the m...

journal_title：Protoplasma

authors： Grigorjeva VV,Gabarayeva N

更新日期：2018-01-01 00:00:00

abstract：:Despite their great economic importance, relatively little is known about bamboo sexual reproduction because they usually spread through rhizomes and have long intervals between flowering periods. Bambusa tuldoides is no exception; the intervals between flowering periods are about 23 years and often do not result in s...

journal_title：Protoplasma

authors： de Souza PF,Dos Santos CMR,Ree J,Guerra MP,Pescador R

更新日期：2020-05-01 00:00:00

abstract：:Formation of intra-molecular disulfides and concomitant oxidative protein folding is essential for stability and catalytic function of many soluble and membrane-bound proteins in the endomembrane system, the mitochondrial inter-membrane space and the thylakoid lumen. Disulfide generation from free cysteines in nascent...

journal_title：Protoplasma

authors： Aller I,Meyer AJ

更新日期：2013-08-01 00:00:00

abstract：:Although rather inconspicuous, movements are an important adaptive trait of plants. Consequently, light- or gravity-induced movements leading to organ bending have been studied intensively. In the field, however, plant movements often result in organ twisting rather than bending. This study investigates the mechanism ...

journal_title：Protoplasma

authors： Borchers A,Deckena M,Buschmann H

更新日期：2018-09-01 00:00:00