The novel functions of kinesin motor proteins in plants.

abstract：:Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based auxin biosensors to m...

journal_title：Protoplasma

authors： Dubas E,Moravčíková J,Libantová J,Matušíková I,Benková E,Zur I,Krzewska M

更新日期：2014-09-01 00:00:00

abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

journal_title：Protoplasma

authors： Tien CJ,Sigee DC,White KN

更新日期：2005-10-01 00:00:00

abstract：:In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...

journal_title：Protoplasma

authors： Lewandowski D,Dubińska-Magiera M,Posyniak E,Rupik W,Daczewska M

更新日期：2017-07-01 00:00:00

abstract：:Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence in the interstitium of mouse testis. Additionally, inactivation of the G-coupled membrane estrogen receptor (GPER) in the testis was performed to obtain insight into TC funct...

journal_title：Protoplasma

authors： Pawlicki P,Hejmej A,Milon A,Lustofin K,Płachno BJ,Tworzydlo W,Gorowska-Wojtowicz E,Pawlicka B,Kotula-Balak M,Bilinska B

更新日期：2019-03-01 00:00:00

abstract：:Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited di...

journal_title：Protoplasma

authors： Kumar J,Gunapati S,Kianian SF,Singh SP

更新日期：2018-09-01 00:00:00

abstract：:Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...

journal_title：Protoplasma

authors： Souza LD,Simioni C,Bouzon ZL,Schneider RC,Gressler P,Miotto MC,Rossi MJ,Rörig LR

更新日期：2017-05-01 00:00:00

abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...

journal_title：Protoplasma

authors： Amnuaykanjanasin A,Epstein L

更新日期：2006-05-01 00:00:00

abstract：:Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...

journal_title：Protoplasma

authors： Duarte VP,Pereira MP,Corrêa FF,de Castro EM,Pereira FJ

更新日期：2021-01-06 00:00:00

abstract：:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...

journal_title：Protoplasma

authors： Farooq S,Fijen C,Hohlbein J

更新日期：2014-03-01 00:00:00

abstract：:The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a flight parabola, an acceleration profile is achieved which yields...

journal_title：Protoplasma

authors： Richter PR,Schuster M,Meyer I,Lebert M,Häder DP

更新日期：2006-12-01 00:00:00

abstract：:Along the past recent years, two-photon excitation (TPE) microscopy has moved from the realms of technical curiosity to be a standard application in many advanced cell biology laboratories. The growing body of literature covered in this review points out the obvious advantages of TPE over any other imaging method base...

journal_title：Protoplasma

authors： Feijó JA,Moreno N

更新日期：2004-03-01 00:00:00

abstract：:Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...

journal_title：Protoplasma

authors： Imani R,Pazoki M,Zupančič D,Kreft ME,Kralj-Iglič V,Veranič P,Iglič A

更新日期：2016-11-01 00:00:00

abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...

journal_title：Protoplasma

authors： Hawkins EK,Lee JJ

更新日期：2001-01-01 00:00:00

abstract：:Although rather inconspicuous, movements are an important adaptive trait of plants. Consequently, light- or gravity-induced movements leading to organ bending have been studied intensively. In the field, however, plant movements often result in organ twisting rather than bending. This study investigates the mechanism ...

journal_title：Protoplasma

authors： Borchers A,Deckena M,Buschmann H

更新日期：2018-09-01 00:00:00

abstract：:In order to corroborate the hypothesis that variations in the rate of spontaneous ultra-weak photon emission (UPE) from germinating seedlings are related to local variations of the lunisolar tidal force, a series of simultaneous tests was performed using the time courses of UPE collected from three plant species-corn,...

journal_title：Protoplasma

authors： Gallep CM,Barlow PW,Burgos RC,van Wijk EP

更新日期：2017-01-01 00:00:00

abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...

journal_title：Protoplasma

authors： Talbot MJ,Offler CE,McCurdy DW

更新日期：2002-05-01 00:00:00

abstract：:The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...

journal_title：Protoplasma

authors： Braun M,Sievers A

更新日期：1993-01-01 00:00:00

abstract：:Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...

journal_title：Protoplasma

authors： Gens JS,Reuzeau C,Doolittle KW,McNally JG,Pickard BG

更新日期：1996-01-01 00:00:00

abstract：:Latex is a complex plant secretion with both ecological and economic importance. There is little information currently available on the cytological aspects of the ontogenesis of anastomosed laticifers, the ducts originating through the lysis of adjacent cell walls. Hancornia speciosa is a tree typical of the Cerrado (...

journal_title：Protoplasma

authors： Gonçalves MP,Mercadante-Simões MO,Ribeiro LM

更新日期：2018-11-01 00:00:00

abstract：:Understanding the mechanisms that microbes exploit to invade host cells and cause disease is crucial if we are to eliminate their threat. Although pathogens use a variety of microbial factors to trigger entry into non-phagocytic cells, their targeting of the host cell process of endocytosis has emerged as a common the...

journal_title：Protoplasma

authors： Lin AE,Guttman JA

更新日期：2010-08-01 00:00:00

abstract：:Titanium dioxide nanoparticles (TiO2-NP) are increasingly being proposed for nanoagriculture but their effect on photosynthesis is limited and contradictory, mostly regarding putative chronical effects associated to the exposure to the commercial P25 formulation (anatase:rutile). This research aims at evaluating how c...

journal_title：Protoplasma

authors： Dias MC,Santos C,Pinto G,Silva AMS,Silva S

更新日期：2019-01-01 00:00:00

abstract：:Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...

journal_title：Protoplasma

authors： Koutalianou M,Orfanidis S,Katsaros C

更新日期：2016-03-01 00:00:00

abstract：:Salinity and drought are important abiotic stresses limiting plant growth and development. Late embryogenesis abundant (LEA) proteins are a group of proteins associated with tolerance to water-related stress. We previously cloned an LEA gene, SmLEA, from Salvia miltiorrhiza Bunge. Phylogenetic analysis indicated that ...

journal_title：Protoplasma

authors： Wu Y,Liu C,Kuang J,Ge Q,Zhang Y,Wang Z

更新日期：2014-09-01 00:00:00

abstract：:Despite more than 130 years of research, phloem loading is far from being understood in gymnosperms. In part this is due to the special architecture of their leaves. They differ from angiosperm leaves among others by having a transfusion tissue between bundle sheath and the axial vascular elements. This article review...

journal_title：Protoplasma

authors： Liesche J,Martens HJ,Schulz A

更新日期：2011-01-01 00:00:00

abstract：:Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...

journal_title：Protoplasma

authors： Ahanger MA,Tittal M,Mir RA,Agarwal RM

更新日期：2017-09-01 00:00:00

abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

journal_title：Protoplasma

authors： Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

更新日期：2006-08-01 00:00:00

abstract：:Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained ...

journal_title：Protoplasma

authors： Ferreira BG,Carneiro RG,Isaias RM

更新日期：2015-09-01 00:00:00

abstract：:Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First, the volume fraction...

journal_title：Protoplasma

authors： Schwuchow JM,Kern VD,Wagner T,Sack FD

更新日期：2000-01-01 00:00:00

abstract：:The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa-P. palustris, P. belevskii, and P. stagnalis-were studied using light and transmission electron microscopy. We applied the periodic acid-Schiff reaction that is a highly selective method for detecting glycogen. Th...

journal_title：Protoplasma

authors： Chistyakova L,Bezborodkina N,Berdieva M,Radaev A,Goodkov A

更新日期：2020-11-01 00:00:00

abstract：:As the by-products of edible oil production with rich lignin, the reserves of Camellia oleifera shell were abundant and had a great economic value. Lignin was the most important limiting factor during the conversion of plant biomass to pulp or biofuels, which mainly deposited in the stone cells of C. oleifera shells. ...

journal_title：Protoplasma

authors： Wang Q,Hu J,Yang T,Chang S

更新日期：2020-10-26 00:00:00